Factors Affecting Lipase Production by Mucor racemosus

1981 ◽  
Vol 44 (9) ◽  
pp. 661-664 ◽  
Author(s):  
A. K. CHOPRA ◽  
HARISH CHANDER ◽  
V. K. BATISH ◽  
B. RANGANATHAN
Keyword(s):  
Enzyme Production ◽  
Sodium Citrate ◽  
Nitrogen Sources ◽  
Lipase Production ◽  
Mucor Racemosus ◽  
Factors Affecting ◽  
Static Culture

Mucor racemosus isolated from butter exhibited maximum lipase production at 22 C in 3 days at pH 5.0, when grown as a static culture. Supplementation of the medium with glucose and lactose at 1% level resulted in maximum (18.0 μmoles FFA) and minimum (10.5 μmoles FFA) enzyme production. Of the five different nitrogen sources tested, peptone at the 2% level supported highest production of lipase. Calcium, potassium and sodium citrate (0.1 %), when incorporated in the medium, stimulated production of enzyme to the extent of 80, 60 and 47%, respectively.

scholarly journals Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production

1993 ◽  
Vol 39 (10) ◽  
pp. 978-981 ◽  
Author(s):  
A. B. Salleh ◽  
R. Musani ◽  
M. Basri ◽  
K. Ampon ◽  
W. M. Z. Yunus ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Corn Steep Liquor ◽  
Carbon Sources ◽  
Extracellular Enzyme ◽  
Lipase Production ◽  
Intracellular Enzyme ◽  
Factors Affecting ◽  
Positive Effects ◽  
Steep Liquor

A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellular lipase. Peptone was the best substrate for extracellular enzyme production, but for intracellular lipase production other substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, glycerol was the only stimulator of extracellular enzyme production, whereas olive oil, triolein, and oleic acid had very positive effects on intracellular enzyme production. Shaking enhanced the production of both types of enzymes; the temperature optima were 45 and 37 °C for extra- and intra-cellular lipases, respectively. A pH of 5.0 was optimal for production of both enzymes.Key words: lipases, Rhizopus oryzae, production.

Factors Affecting Protease Production by Bacillus stearothermophilus RM-67

1983 ◽  
Vol 46 (12) ◽  
pp. 1020-1025 ◽  
Author(s):  
A. K. CHOPRA ◽  
D. K. MATHUR
Keyword(s):  
Yeast Extract ◽  
Enzyme Production ◽  
Bacillus Stearothermophilus ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Protease Production ◽  
Salt Medium ◽  
Medium Ph ◽  
Factors Affecting ◽  
Rotary Shaker

Amongst the nitrogen sources, tryptone and yeast extract at 0.5% and 0.15% level, respectively, caused maximum enzyme production by Bacillus stearothermophilus RM-67. Addition of sodium chloride (0.5%) to the basal medium enhanced the enzyme production by 63%. Various sugars incorporated into the standardized basal medium proved inhibitory to enzyme elaboration. Maximum enzyme production was observed in the early decline growth phase of the organism in tryptone-yeast extract-salt medium (pH 6.5) when inoculated at 4% level and incubated on a rotary shaker at 55°C for 8 h and subsequently at 45°C up to 24 h.

Nitrification: an old process, a new concern

1971 ◽  
Vol 24 ◽  
Author(s):  
W. H. Verstraete
Keyword(s):  
Culture Medium ◽  
Nitrogen Sources ◽  
Living Cells ◽  
Factors Affecting ◽  
Inhibiting Effect ◽  
Proteose Peptone ◽  
Asparaginase Activity

Some  factors affecting the L-asparaginase activity of E.  aroideae were investigated. Increasing  concentrations of glucose in the culture medium had an inhibiting effect on  the production of L-asparaginase by this microorganism. Buffering of the  culture medium in order to stabilize the pH during growth resulted in a decrease  of the L-asparaginase activity. From the different nitrogen sources examined,  tryptone, proteose peptone nr 2 and nr 3 stimulated the L-asparaginase  production. Toluene treatment of the cells practically destroyed the  L-asparaginase. Acetone dried cells showed an L-asparaginase activity  comparable with the activity of living cells.

FACTORS AFFECTING LIPASE PRODUCTION BY PENICILLIUM CHRYSOGENUM

1977 ◽  
Vol 42 (6) ◽  
pp. 1677-1677 ◽  
Author(s):  
HARISH CHANDER ◽  
S. S. SANNABHADTI ◽  
JOB ELIAS ◽  
B. RANGANATHAN
Keyword(s):  
Penicillium Chrysogenum ◽  
Lipase Production ◽  
Factors Affecting

scholarly journals Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
S. P. Gautam ◽  
P. S. Bundela ◽  
A. K. Pandey ◽  
Jamaluddin Khan ◽  
M. K. Awasthi ◽  
...  
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Enzyme Production ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Production Medium ◽  
Optimum Temperature ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Alternative Carbon Source

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

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