scholarly journals A slide preparation technique for light microscopy analysis of ciliates preserved in acid Lugol's fixative

2014 ◽  
Vol 12 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Alexandra Freibott ◽  
Lorena Linacre ◽  
Michael R. Landry
Keyword(s):  
Light Microscopy ◽  
Preparation Technique ◽  
Slide Preparation ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis

High throughput solubility measurement with automated polarized light microscopy analysis

2006 ◽  
Vol 95 (10) ◽  
pp. 2115-2122 ◽  
Author(s):  
Kiyohiko Sugano ◽  
Teruhisa Kato ◽  
Kentaro Suzuki ◽  
Kako Keiko ◽  
Tetsujo Sujaku ◽  
...  
Keyword(s):  
Light Microscopy ◽  
High Throughput ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Solubility Measurement ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis

Novel Quantitative Method to Evaluate Globotriaosylceramide Inclusions in Renal Peritubular Capillaries by Virtual Microscopy in Patients With Fabry Disease

2012 ◽  
Vol 136 (7) ◽  
pp. 816-824 ◽  
Author(s):  
Laura Barisoni ◽  
J. Charles Jennette ◽  
Robert Colvin ◽  
Sheela Sitaraman ◽  
Alexander Bragat ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Fabry Disease ◽  
Scoring System ◽  
Quantitative Method ◽  
Virtual Microscopy ◽  
Lipid Inclusion ◽  
Peritubular Capillaries ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis ◽  
Conventional Light Microscopy

Context.—Assessing the amount of globotriaosylceramide inclusions in renal peritubular capillaries by a semiquantitative approach is a standard and useful measure of therapeutic efficacy in Fabry disease, achievable by light microscopy analysis. Objective.—To describe a novel virtual microscopy quantitative method to measure globotriaosylceramide inclusions (Barisoni Lipid Inclusion Scoring System [BLISS]) in renal biopsies from patients with Fabry disease. Design.—Plastic embedded 1-µm-thick sections from kidney biopsies from 17 patients enrolled in a Fabry disease clinical trial were evaluated using a standard semiquantitative methodology and BLISS to compare sensitivity. We also tested intrareader and interreader variability of BLISS and compared results from conventional light microscopy analysis with a virtual microscopy-based methodology. Peritubular capillaries were first annotated on digital images of whole slides by 1 pathologist and then scored for globotriaosylceramide inclusions by 2 additional pathologists. Results.—We demonstrated that (1) quantitative analysis by BLISS results in detection of small amount of globotriaosylceramide inclusions even when by semiquantitative analysis the score is 0, (2) application of BLISS combined with conventional light microscopy results in low intrareader and interreader variability, and (3) BLISS combined with virtual microscopy results in significant reduction of intrareader and interreader variability compared with BLISS–light microscopy. Conclusions.—BLISS is a simpler and more sensitive scoring system compared to the semiquantitative approach. The virtual microscopy–based methodology increases accuracy and reproducibility; moreover, it provides a permanent record of retrievable data with full transparency in clinical trials.

The Occurrence of Phase Separation in Microcrystalline Cellulose Filled Natural Rubber Films

2016 ◽  
Vol 1133 ◽  
pp. 196-200
Author(s):  
Ruslimie Che Ali ◽  
Asrul Mustafa
Keyword(s):  
Phase Separation ◽  
Light Microscopy ◽  
Natural Rubber ◽  
Microcrystalline Cellulose ◽  
Cross Sections ◽  
Natural Rubber Latex ◽  
Rubber Matrix ◽  
High Ammonia ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis

This paper describes the use of microcrystalline cellulose (MCC) as hydrophilic filler in two types of natural rubber latexes which are high ammonia natural rubber (HA) and epoxidised natural rubber latex (ENR). Light microscopy (LM), Scanning electron microscopy (SEM) and tensile strength measurements were then conducted on the resulting films. The light microscopy analysis revealed that more MCC particles appeared at the air–facing (AF) surfaces relative to the substrate-facing (SF) surfaces of the HA films with increasing MCC content. In contrary, the ENR25 films showed more or less similar MCC particles appearing at both air-facing surfaces and substrate-facing surfaces. Further SEM cryo-fracture analysis at the cross sections of the MCC filled ENR25 films indicated that the MCC particles were randomly dispersed in the rubber matrix. It can be suggested that the phase separation of MCC particles to the films surfaces occurred due to the incompatibility of MCC with the rubber matrix. Thus, it can be inferred that the occurrence of phase separation is minimised in the MCC filled ENR films in comparison to the MCC filled HA films.

Ultrastructure and distribution of Urosolenia obesa sp. nov. (Rhizosoleniaceae, Diatomeae) in reservoirs from southern Brazil

Phytotaxa ◽  
2013 ◽  
Vol 125 (1) ◽  
pp. 1 ◽  
Author(s):  
PRISCILA I. TREMARIN ◽  
EDUARDO G. FREIRE ◽  
THELMA A. V. LUDWIG
Keyword(s):  
New Species ◽  
Light Microscopy ◽  
Geographic Distribution ◽  
The Other ◽  
Similar Species ◽  
Southern Brazil ◽  
Oblique Angle ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis ◽  
Planktonic Species

A new planktonic species of Urosolenia is described from Southern Brazilian reservoirs. Urosolenia obesa sp. nov. was found in several studied samples and the unique set of features led us to propose it as new and to compare their morphology with similar species found in the literature, as Rhizosolenia  naui, R. eriensis var. pusilla and Urosolenia parva. Urosolenia obesa differs from the other species mainly by sub-cylindrical frustules, conical valves, calyptra straight, positioned in right to oblique angle in relation to pervalvar axis, ornamented by longitudinal ribs and ending in two teeth and one ligula. Probably, the new species has a wider geographic distribution, since the small size and slightly silicified frustules make it difficult to visualize them by light microscopy analysis of samples.

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