scholarly journals Infección mixta por Plasmodium falciparum y Plasmodium malariae: Valor de las técnicas de diagnóstico molecular

2008 ◽  
Vol 25 (3) ◽  
Author(s):  
J. Campos Franco ◽  
J. Llovo Taboada ◽  
R. López Rodríguez ◽  
N. Mallo González ◽  
S. Cortizo Vidal ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Malariae

The Blood-stage Dynamics of Mixed Plasmodium malariae–Plasmodium falciparum Infections

1999 ◽  
Vol 198 (4) ◽  
pp. 549-566 ◽  
Author(s):  
Daniel P Mason ◽  
F.Ellis McKenzie ◽  
William H Bossert
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Malariae ◽  
Blood Stage

scholarly journals Persistent transmission of Plasmodium malariae and Plasmodium ovale species in an area of declining Plasmodium falciparum transmission in eastern Tanzania

2019 ◽  
Vol 13 (5) ◽  
pp. e0007414 ◽  
Author(s):  
Victor Yman ◽  
Grace Wandell ◽  
Doreen D. Mutemi ◽  
Aurelie Miglar ◽  
Muhammad Asghar ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Malariae ◽  
Plasmodium Ovale

scholarly journals Plasmodium falciparum DHFR and DHPS Mutations Are Associated With HIV-1 Co-Infection and a Novel DHPS Mutation I504T Is Identified in Western Kenya

2020 ◽  
Vol 10 ◽  
Author(s):  
Brandi K. Torrevillas ◽  
Sarah M. Garrison ◽  
Alexander J. McKeeken ◽  
Dharmeshkumar Patel ◽  
James T. Van Leuven ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Haplotype Diversity ◽  
Plasmodium Malariae ◽  
Drug Binding ◽  
Cross Resistance ◽  
Asymptomatic Malaria ◽  
Newly Diagnosed ◽  
Cross Sectional ◽  
Antifolate Resistance ◽  
Hiv 1

Antifolate resistance is significant in Kenya and presumed to result from extensive use and cross-resistance between antifolate antimalarials and antibiotics, including cotrimoxazole/Bactrim used for HIV-1 chemotherapy. However, little is known about antifolate-resistant malaria in the context of newly diagnosed HIV-1 co-infection prior to administration of HIV-1 chemotherapy. Blood samples from a cross-sectional study of asymptomatic adult Kenyans enrolled during voluntary HIV testing were analyzed by PCR for Plasmodium spp. More than 95% of volunteers with identifiable parasite species (132 HIV-1 co-infected) were infected with Plasmodium falciparum alone or P. falciparum with Plasmodium ovale and/or Plasmodium malariae. Deep sequencing was used to screen for mutations in P. falciparum dihydrofolate reductase (dhfr) (N51I, C59R, S108N, I164L) and dihydropteroate synthase (dhps) (S436H, A437G, K540E, A581G) from 1133 volunteers. Individual mutations in DHPS but not DHFR correlated with HIV-1 status. DHFR haplotype diversity was significantly different among volunteers by gender and HIV-1 status. DHPS haplotype diversity by HIV-1 status was significantly different between volunteers paired by age and gender, indicating that patterns of resistance were independent of these variables. Molecular simulations for a novel DHPS mutation (I504T) suggested that the mutated protein has increased affinity for the endogenous ligand DHPPP and decreased affinity for drug binding. A sub-group of monoclonal infections revealed that age and parasitemia were not correlated and enabled identification of a rare septuple-mutant haplotype (IRNL-HGEA). In our study, adult Kenyans newly diagnosed with HIV-1 infection were predominantly infected with moderately resistant P. falciparum, with patterns of infecting parasite genotypes significantly associated with HIV-1 status. Together with the discovery of DHPS I504T, these data indicate that antifolate resistance continues to evolve in Kenya. Further, they highlight the need to understand the effects of associated mutations on both fitness and resistance of P. falciparum in the context of HIV-1 co-infection to better inform treatment for asymptomatic malaria.

scholarly journals Malária em região extra-Amazônica: situação no Estado de Santa Catarina

2003 ◽  
Vol 36 (5) ◽  
pp. 581-586 ◽  
Author(s):  
Ricardo Luiz Dantas Machado ◽  
Álvaro Augusto Ribeiro D' Almeida Couto ◽  
Carlos Eugênio Cavasini ◽  
Vanja Sueli Pachiano Calvosa
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Plasmodium Malariae ◽  
Santa Catarina

Este estudo objetiva conhecer o perfil epidemiológico da malária no Estado de Santa Catarina, analisando dados disponibilizados pela Fundação Nacional de Saúde, relativos ao período de 1996/2001. Das 4.707 lâminas examinadas, 5,5% evidenciaram-se positivas. As infecções por Plasmodium vivax foram 69%, por Plasmodium falciparum 25,6%, infecções mistas por ambos foram 5% e, somente 0,4% por Plasmodium malariae. Foi observado 67,4% casos importados e 32,6% casos autóctones. Nos últimos anos houve um aumento de casos importados. A maioria destes veio da região Amazônica brasileira e o restante de países africanos. Identificou-se os municípios de Joinville, Blumenau, São Francisco do Sul e Florianópolis com maior número de autoctonia no biênio 1996/97. Medidas de controle e vigilância fazem-se necessárias, no sentido de prevenir a reintrodução do plasmódio, favorecendo a autoctonia. Será útil o mapeamento das áreas de risco, já que é contínua a expectativa de sua reemergência em áreas hoje consideradas sob controle.

scholarly journals Diagnóstico molecular da malária em uma unidade de atenção terciária na Amazônia Brasileira

2008 ◽  
Vol 41 (4) ◽  
pp. 381-385 ◽  
Author(s):  
Mônica Regina Farias Costa ◽  
Pedro Paulo Ribeiro Vieira ◽  
Cynthia de Oliveira Ferreira ◽  
Marcus Vinícius Guimarães de Lacerda ◽  
Wilson Duarte Alecrim ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Nested Pcr ◽  
Plasmodium Malariae

O exame de rotina para o diagnóstico da malária continua sendo a gota espessa, apesar da comprovada diminuição da sensibilidade e especificidade em situações de densidade parasitária baixa e infecções mistas. A reação em cadeia da polimerase vem sendo cada vez mais utilizada para a detecção molecular e identificação das espécies de plasmódio, por apresentar maior sensibilidade e especificidade. Foi realizada a nested-PCR em amostras de sangue total de 344 pacientes com síndrome febril aguda que se apresentaram para o diagnóstico de malária, em uma unidade terciária de saúde, em Manaus (Amazonas). Nenhum caso de malária por Plasmodium malariae foi diagnosticado à gota espessa ou PCR. Observou-se co-positividade de 96,7%, co-negatividade de 62,2% e coeficiente kappa de 0,44 entre PCR e gota espessa para Plasmodium falciparum. Para Plasmodium vivax, co-positividade de 100%, co-negatividade de 78,1% e coeficiente kappa de 0,56. Na detecção da malária mista, co-positividade de 100%, co-negatividade de 84,9% e coeficiente kappa de 0,26. A reação em cadeia da polimerase detectou alto número de infecções mistas nas amostras analisadas, mas seu uso rotineiro no diagnóstico da malária merece ainda ampla discussão.

scholarly journals Limited Polymorphism of the Kelch Propeller Domain in Plasmodium malariae and P. ovale Isolates from Thailand

2016 ◽  
Vol 60 (7) ◽  
pp. 4055-4062 ◽  
Author(s):  
Supatchara Nakeesathit ◽  
Naowarat Saralamba ◽  
Sasithon Pukrittayakamee ◽  
Arjen Dondorp ◽  
Francois Nosten ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Southeast Asia ◽  
Severe Malaria ◽  
Malaria Control ◽  
Artemisinin Resistance ◽  
Plasmodium Malariae ◽  
The Other ◽  
Content Type ◽  
Functional Consequences ◽  
First Time

ABSTRACTArtemisinin resistance inPlasmodium falciparum, the agent of severe malaria, is currently a major obstacle to malaria control in Southeast Asia. A gene named “kelch13” has been associated with artemisinin resistance inP. falciparum. The orthologue of thekelchgene inP. vivaxwas identified and a small number of mutations were found in previous studies. Thekelchorthologues in the other two human malaria parasites,P. malariaeandP. ovale, have not yet been studied. Therefore, in this study, the orthologouskelchgenes ofP. malariae,P. ovale wallikeri, andP. ovale curtisiwere isolated and analyzed for the first time. The homologies of thekelchgenes ofP. malariaeandP. ovalewere 84.8% and 82.7%, respectively, compared to the gene inP. falciparum.kelchpolymorphisms were studied in 13P. malariaeand 5P. ovaleisolates from Thailand. There were 2 nonsynonymous mutations found in these samples. One mutation was P533L, which was found in 1 of 13P. malariaeisolates, and the other was K137R, found in 1 isolate ofP. ovale wallikeri(n= 4). This result needs to be considered in the context of widespread artemisinin used within the region; their functional consequences for artemisinin sensitivity inP. malariaeandP. ovalewill need to be elucidated.

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