Determination of Affinity Constants by Partition Equilibrium Methods

2021 ◽  
pp. 55-77
Author(s):  
Donald J. Winzor
Keyword(s):  
Affinity Constants ◽  
Partition Equilibrium

Low Affinity Calcium Binding Sites of the Calcium Transport ATPase of Sarcoplasmic Reticulum Membranes

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1012-1018 ◽  
Author(s):  
Wilhelm Hasselbach ◽  
Vera Koenig
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Calcium Binding ◽  
Calcium Transport ◽  
External Surface ◽  
Transport Atpase ◽  
Affinity Constants ◽  
Calcium Binding Sites ◽  
Short Time

Calcium binding sites having low affinity constants of < 103 ᴍ-1 were titrated in native sarcoplasmic reticulum vesicles as well as in lipid deprived membranes and in the isolated calcium transport ATPase. Short time calcium binding measurements and the determination of the calcium binding heat allow to distinguish low affinity calcium binding sites located on the external surface of the sarcoplasmic reticulum membranes from those present in the section of the transport molecule directed to the vesicular space. The same number of internal binding sites was found for preparations deprived of their lipid content as well as of preparations depleted of their lipids and of their accessorial proteins. Magnesium interferes with calcium binding to the external as well as to the internal low affinity calcium binding sites. The implications of the existence of the low affinity calcium binding sites in the internal section of the calcium transport ATPase are discussed.

Determination of Receptor–Ligand Affinity Constants

2021 ◽  
pp. 121-131
Author(s):  
Gerhard T. Viel ◽  
Kees Ensing ◽  
Rokus A. de Zeeuw
Keyword(s):  
Receptor Ligand ◽  
Ligand Affinity ◽  
Affinity Constants

Comparison of three distinct ELLA protocols for determination of apparent affinity constants between Con A and glycoproteins

2012 ◽  
Vol 94 ◽  
pp. 163-169 ◽  
Author(s):  
D. Mislovičová ◽  
J. Katrlík ◽  
E. Paulovičová ◽  
P. Gemeiner ◽  
J. Tkac
Keyword(s):  
Con A ◽  
Apparent Affinity ◽  
Affinity Constants
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