scholarly journals Differentiation of Human Deceased Donor, Adipose-Derived, Mesenchymal Stem Cells into Functional Beta Cells

2020 ◽  
Vol 16 (2) ◽  
pp. 63-72
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Beta Cells ◽  
Deceased Donor

Dysregulated lncRNAs Regulate Human Umbilical Cord Mesenchymal Stem Cells Differentiation into Insulin-Producing Cells by Forming a Regulatory Network with mRNAs

2021 ◽  
Author(s):  
Tianqin Xie ◽  
Qiming Huang ◽  
Qiulang Huang ◽  
Haixia Zeng ◽  
Jianping Liu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Insulin Secretion ◽  
Signaling Pathway ◽  
Beta Cells ◽  
Umbilical Cord ◽  
Pancreatic Beta Cells ◽  
Differentially Expressed ◽  
Human Umbilical Cord ◽  
Insulin Producing Cells

Abstract ObjectiveIn recent years, cell therapy has become a new research direction in the treatment of diabetes. However, the underlying molecular mechanisms of mesenchymal stem cells (MSCs) participate in such treatment has not been clarified. MethodsIn this study, human umbilical cord mesenchymal stem cells (HUC-MSCs) isolated from newborns were progressively induced into insulin-producing cells (IPCs) using small molecules. HUC-MSCs (S0) and four induced stage (S1-S4) samples were prepared. We then performed transcriptome sequencing experiments to obtain the dynamic expression profiles of both mRNAs and long noncoding RNAs (lncRNAs). ResultsWe found that the number of differentially expressed lncRNAs and mRNAs showed a decreasing trend during differentiation. Gene Ontology (GO) analysis showed that the target genes of differentially expressed lncRNAs were associated with translation, cell adhesion, and cell connection. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the NF-KB signaling pathway, MAPK signaling pathway, HIPPO signaling pathway, PI3K-Akt signaling pathway, and p53 signaling pathway were enriched in these differentially expressed lncRNA-targeting genes. We also found that the coexpression of the lncRNA: CTBP1-AS2 with the PROX1, and the lncRNAs AC009014.3 and GS1-72M22.1 with the mRNA JARID2 was related to the development of pancreatic beta cells. Moreover, the coexpression of the lncRNAs :XLOC_ 050969, LINC00883, XLOC_050981, XLOC_050925, MAP3K14- AS1, RP11-148K1.12, and CTD2020K17.3 with p53, regulated insulin secretion by pancreatic beta cells.ConclusionThis research revealed that HUC-MSCs combined with small molecule compounds were successfully induced into IPCs. Differentially expressed lncRNAs may regulate the insulin secretion of pancreatic beta cells by regulating multiple signaling pathways. The lncRNAs: AC009014.3,Gs1-72m21.1 and CTBP1-AS2 may be involved in the development of pancreatic beta cells, and the lncRNAs: XLOC_050969, LINC00883, XLOC_050981, XLOC_050925, MAP3K14-AS1, RP11-148K1.12, and CTD2020K17.3 may be involved in regulating the insulin secretion of pancreatic beta cells, thus providing a lncRNA catalog for future research regarding the mechanism of the transdifferentiation of HUC-MSCs into IPCs. It also provides a new theoretical basis for the transplantation of insulin-producing cells into diabetic patients in the future.

scholarly journals An overview on small molecule-induced differentiation of mesenchymal stem cells into beta cells for diabetic therapy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nimshitha Pavathuparambil Abdul Manaph ◽  
Kisha N. Sivanathan ◽  
Jodie Nitschke ◽  
Xin-Fu Zhou ◽  
Patrick T. Coates ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Beta Cell ◽  
Small Molecules ◽  
Beta Cells ◽  
Small Molecule ◽  
Pancreatic Beta Cells ◽  
Mature Beta Cells

Abstract The field of regenerative medicine provides enormous opportunities for generating beta cells from different stem cell sources for cellular therapy. Even though insulin-secreting cells can be generated from a variety of stem cell types like pluripotent stem cells and embryonic stem cells, the ideal functional cells should be generated from patients’ own cells and expanded to considerable levels by non-integrative culture techniques. In terms of the ease of isolation, plasticity, and clinical translation to generate autologous cells, mesenchymal stem cell stands superior. Furthermore, small molecules offer a great advantage in terms of generating functional beta cells from stem cells. Research suggests that most of the mesenchymal stem cell-based protocols to generate pancreatic beta cells have small molecules in their cocktail. However, most of the protocols generate cells that mimic the characteristics of human beta cells, thereby generating “beta cell-like cells” as opposed to mature beta cells. Diabetic therapy becomes feasible only when there are robust, functional, and safe cells for replacing the damaged or lost beta cells. In this review, we discuss the current protocols used to generate beta cells from mesenchymal cells, with emphasis on small molecule-mediated conversion into insulin-producing beta cell-like cells. Our data and the data presented from the references within this review would suggest that although mesenchymal stem cells are an attractive cell type for cell therapy they are not readily converted into functional mature beta cells.

scholarly journals Differentiation of conjunctiva mesenchymal stem cells into secreting islet beta cells on plasma treated electrospun nanofibrous scaffold

2017 ◽  
Vol 46 (sup1) ◽  
pp. 178-187 ◽  
Author(s):  
Samad Nadri ◽  
Ghasem Barati ◽  
Hossein Mostafavi ◽  
Abdolreza Esmaeilzadeh ◽  
Seyed Ehsan Enderami
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Beta Cells ◽  
Nanofibrous Scaffold ◽  
Islet Beta Cells

scholarly journals DIFFERENTIATION OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING BETA CELLS FOR DIABETIC THERAPY.

2017 ◽  
Vol 5 (5) ◽  
pp. 1875-1882
Author(s):  
KeerthiKonda Juturu ◽  
◽  
Vasundhara Kamineni ◽  
Ravi Shankar ◽  
Jayashankar Errukumbattu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Beta Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord
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