scholarly journals FIRST OUTBREAK OF LACTOCOCCOSIS CAUSED BY LACTOCOCCUS GARVIEAE IN SERBIA

2020 ◽  
Vol 13 (1) ◽  
pp. 53-68
Author(s):  
Vladimir Radosavljević ◽  
Oliver Radanović ◽  
Nemanja Zdravković ◽  
Božidar Savić ◽  
Marko Stanković ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Cumulative Mortality ◽  
Lactococcus Garvieae ◽  
Isolation And Characterization ◽  
First Case ◽  
Diseased Fish ◽  
Gram Positive Cocci

The outbreak of lactococcosis affecting rainbow trout, weighing 70 - 120 g, occurred at a trout aquaculture facility in Central-West Serbia during July, 2018. This outbreak lasted for three weeks, and cumulative mortality attributed to the disease was around 40%. The diseased fish showed erratic swimming, dark discoloration and exophthalmia, with the cumulative mortality of around 40%. A pure Gram-positive cocci isolates were obtained from the eye and kidney samples. Based on colony morphology, phenotypic and biochemical characteristics, the isolated bacterium was presumably identified as Lactococcus garvieae. Using the BBL CRYSTAL GP ID system, the isolate was identified as L. garvieae and the identity of the isolate was confirmed with MALDI-TOF Mass Spectrometry. Blast analysis of 16S rRNA sequence of our isolate had a 99.4 to 99.6% similarity to the L. garvieae strains previously isolated from diseased fish. The antibacterial activity of 15 antimicrobials against L. garvieae was evaluated using disc diffusion. In this paper, we report the first case of lactococcosis in rainbow trout in Serbia, isolation and characterization of causative agent, Lactococcus garvieae from diseased rainbow trout.

Isolation and characterization of Lactococcus garvieae from rainbow trout , Onchorhyncus mykiss, from California, USA

2021 ◽  
Author(s):  
Khalid Shahin ◽  
Tresa Veek ◽  
Taylor I. Heckman ◽  
Eric Littman ◽  
Kaveramma Mukkatira ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Lactococcus Garvieae ◽  
Isolation And Characterization

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Sign in / Sign up

Export Citation Format

Share Document