scholarly journals A Pharmacognostical and Phytochemical study of Sookshma Eladi Choornam

2020 ◽  
Vol 11 (4) ◽  
pp. 712-715
Author(s):  
Harinatha Chary B ◽  
Manu Rajagopal ◽  
Pavan Kumar S ◽  
Gnana Prasuna S
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Fine Powder ◽  
Chemical Study ◽  
Quality Parameters ◽  
Water Soluble ◽  
Dissolution Time ◽  
Phytochemical Study ◽  
Layer Chromatography

Introduction: Sookshma Eladi choornam is one of the Ayurvedic formulations specially mentioned for Kaphaja Hridroga in Hridroga prakarana of Bhaishajya Ratnavali.  Dhamani pratichaya (Atherosclerosis) is one of the diseases of Kaphaja nanatmaja vaydhi which is the more important cause for Ischemic Heart Disease. The cause for Atherosclerosis could be Hyperlipidemias. Eladi choornam is a compound preparation which contains powders of Sookshma ela (Elettaria cardamomum Maton.) and Pippali moolam (root of Piper longum L.,). As these drugs are Katu vipaka in nature they can be used in Hyerlipidemia.  To know the efficacy of the drugs their quality parameters are highly essential to manufacture in the large scale. Method: The present study deals with the Pharmacognostical and phyto-chemical study of Sookshma eladi choornam including Thin Layer chromatography study (TLC) as per the standard literature. Result: Consistency of Sookshma eladi choornam is fine powder. Colour was brown, odour was aromatic spicy smell, Taste was pungent, touch was smooth powder form,  Qualitative study showed that pH is 4.9, total ash value 12 %, loss on drying is 4.5%, Water soluble matter17%, Alcohol soluble matter 8%,  Acid insoluble ash 8.5%. Thin Layer chromatography (TLC) revealed one yellow spot.  Dissolution time is 4 minutes and Moisture content was 8%. Phytochemicals as Alkaloids, Glycosides, Tannins, Flavonoids and Phenols were found. Conclusion: Pharmacognostical, phyto-chemical and TLC studies inferred that the formulation meets the minimum quality standards. The study may be used as reference standard in the further quality control researches.

scholarly journals Thin Layer Chromatography of Dyes. I : The Frequency in Use of Water Soluble Dyes Containing in Commercial Drugs.

Eisei kagaku ◽  
1967 ◽  
Vol 12 (6) ◽  
pp. 384-385
Author(s):  
Fukujiro Fujikawa ◽  
Kunio Hirai ◽  
Misuzu Tachibana ◽  
Utaka Otani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Layer Chromatography

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals Separation of some vitamins in reversed-phase thin-layer chromatography and pressurized planar electrochromatography with eluent containing surfactant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beata Polak ◽  
Emilia Pajurek
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Theoretical Plate ◽  
Reversed Phase ◽  
Water Soluble ◽  
System Capacity ◽  
Micellar Systems ◽  
Layer Chromatography

AbstractThe separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research. Hence, the influence of the mobile phase composition (surfactant and acetonitrile concentration, eluent buffer pH) on the migration distances and zone separation of some vitamins (thiamine, riboflavin, niacin, pyridoxine, cyanocobalamin, folic acid, ergocalciferol and α-tocopherol) was investigated. Our results indicated that the applied technique has an impact on the solute order. Comparing the system capacity of HPLC and PPEC (measured as height of the theoretical plate) for the mobile phase systems with and without surfactant shows differences, especially for fat-soluble vitamin. The variances and reproducibilities (% RDS) values of the vitamin are less in PPEC than in TLC. Moreover, the migration distances of water-soluble vitamins are longer than fat-soluble ones. Overall, eluent consisting of 50% acetonitrile, 18.75 mM SDS, the buffer of pH 6.99 via the PPEC technique was most appropriate for determining the investigated vitamins in the artificial mixture and the two commercially available vitamin combinations.

Micro-analysis for quinidine in serum by thin-layer chromatography followed by fluorescence densitometry.

1983 ◽  
Vol 29 (12) ◽  
pp. 2100-2102 ◽  
Author(s):  
M Kelner ◽  
D N Bailey
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Internal Standard ◽  
Peak Height ◽  
Peak Height Ratio ◽  
Height Ratio ◽  
Coefficients Of Variation ◽  
Micro Analysis ◽  
Layer Chromatography

Abstract We report a thin-layer-chromatographic micro-analysis for quinidine in serum, with detection by fluorescence densitometry. Quinidine is extracted from 20 microL of serum at pH 13 into 3 mL of hexane/acetone solution (80/20 by vol) containing N-(1-naphthyl)ethylenediamine as internal standard. The extract is concentrated and applied to silica-gel-impregnated plates for conventional thin-layer chromatography. Quinidine is identified from its RF value and quantified from the peak-height ratio between quinidine and the internal standard, relative to that of simultaneously extracted serum standards. The proposed assay is sensitive (to 0.2 mg/L), specific for unmetabolized quinidine, precise (between-run coefficients of variation less than 6%), and readily adaptable to large-scale "batch" analysis. Peak-height ratio is linearly related to concentration to at least 20 mg/L. Quinidine concentrations in the serum of patients, as measured by the proposed method (x) and by a traditional double-extraction spectrofluorometric assay (y), were related as follows: y = 0.994x + 0.276 (r = 0.989, n = 20).

Qualitative analysis of the herb fagopyrum sagittatum gilib grown in pot culture

2020 ◽  
pp. 63-68
Author(s):  
A. S. Soldatov ◽  
N. V. Nesterova ◽  
N. V. Biryukova
Keyword(s):  
Qualitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phytochemical Study ◽  
Promising Source ◽  
Layer Chromatography ◽  
Pot Culture

This article is devoted to the phytochemical study of fresh and dried samples of buckwheat grass, the preparation of which was carried out from plants cultivated in pot culture using pharmacopoeial qualitative reactions. The presence of tannins and flavonoids was identified, and using the method of thin-layer chromatography, using GSO Rutin, rutin was identified, which allows us to consider potted buckwheat culture as a promising source of rutin production.

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