Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea

1998 ◽  
Keyword(s):  
Ascorbic Acid ◽  
Lake Michigan ◽  
Coho Salmon ◽  
Life Stage ◽  
Dehydroascorbic Acid ◽  
Early Life Stage ◽  
High Survival ◽  
Survival Group ◽  
Chromatography Analysis ◽  
Early Mortality Syndrome

<em>Abstract.—</em>Muscle and egg samples from returning adult female Lake Michigan coho salmon <em>Oncorhynchus kisutch </em>were collected for thiamine analysis. Three groups of five females having low (2.5%), medium (42.4%), or high (92.6%) mean fry survival were selected for this study. Egg and muscle samples were collected at spawning and analyzed by high-performance liquid chromatography analysis for free thiamine, thiamine monophosphate (TP), and thiamine pyrophosphate (TPP). Egg concentrations of ascorbic acid, iron, zinc, magnesium, and potassium were measured. Twenty-five contaminants were also measured in muscle tissue of adult females. Total thiamine levels in eggs were similar between the medium and high survival groups but significantly lower in the low survival group. Eggs from the high and medium survival groups had higher levels of free thiamine and TP (<EM>P </EM>< 0.01) than eggs from the low survival group. There were no significant differences among the three groups in egg TPP. Muscle concentrations of TPP, TP, and total thiamine were similar among the three survival groups (<EM>P </EM>> 0.10). Correlations between fry survival and egg free thiamine (<em>r </em>= 0.61) and TP (<em>r </em>= 0.52) were observed. Fry survival was not correlated with adult muscle concentration of any form of thiamine or contaminant measured. Among the three groups, no differences in egg concentration were found for ascorbic acid, dehydroascorbic acid, iron, magnesium, zinc, and potassium. This research supports the hypothesis that low egg thiamine is an important factor in early mortality syndrome.

Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea

1998 ◽  
Keyword(s):  
Lake Michigan ◽  
Coho Salmon ◽  
Life Stage ◽  
Early Life Stage ◽  
Early Mortality ◽  
Thiamine Hydrochloride ◽  
Predisposing Factor ◽  
Early Mortality Syndrome ◽  
Salmon Eggs ◽  
The Mean

<em>Abstract</em>.—Lake Michigan Skamania strain steelhead <em>Oncorhynchus mykiss </em>and coho salmon <em>O. kisutch </em>fry exhibit an early mortality syndrome (EMS) in which death is preceded by loss of equilibrium, inability or lack of feeding, and general lethargy. It was hypothesized that decreased egg concentrations of carotenoid pigments, thyroxine, or thiamine contributed to this syndrome. Thiamine analyses of Lake Michigan coho salmon eggs from individual family groups exhibited 16–97% EMS if egg total thiamine concentrations were less than 0.9 nmol/g or egg free thiamine concentrations were less than 0.3 nmol/g. In eggs with total or free thiamine concentrations greater than 0.9 or 0.3 nmol/g, respectively, the range of EMS in fry was 5–12%. Immersion of steelhead and coho salmon eggs in 1.4 mM or greater thiamine hydrochloride significantly decreased EMS compared with controls. Immersion of coho salmon eggs in 2.8 mM thiamine increased the mean concentration of free thiamine in the eggs to 1.0 nmol/g, compared with 0.26 nmol/g in controls. Injection of either β-carotene or astaxanthin (0.86 or 8.6 µg/g, respectively) in steelhead eggs did not significantly reduce the occurrence of EMS. Early mortality syndrome was not decreased in steelhead after immersion of eggs in 2 mg/L thyroxine, but it was significantly decreased in steelhead sac fry immersed in 2 mg/L thyroxine. These results suggest that low egg thiamine is a predisposing factor for EMS; however, other factors that are variable among individual coho salmon females may influence the occurrence of EMS-related mortality. Whether the addition of exogenous thiamine corrects a thiamine deficiency or protects fry from developing EMS through some other mechanism is currently unknown.

Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea

1998 ◽  
Keyword(s):  
Thiamine Deficiency ◽  
Lake Michigan ◽  
Lake Trout ◽  
Control Diet ◽  
Lake Superior ◽  
Life Stage ◽  
Early Life Stage ◽  
Alosa Pseudoharengus ◽  
Commercial Diet ◽  
Early Mortality Syndrome

<em>Abstract</em>.—Juvenile and adult lake trout <em>Salvelinus namaycush </em>that were fed semipurified, thiaminedeficient diets or alewives <em>Alosa pseudoharengus </em>containing thiaminase, a thiamine-destroying enzyme, showed no overt symptoms of thiamine deficiency. Growth rates and ovulation rates were similar among all treatments. However, liver thiamine pyrophosphate (TPP), a biochemical indicator of impending thiamine deficiency, in juvenile lake trout fed thiamine-deficient diets was reduced to 35 pmol/ g compared with 59 pmol/g in control groups. Blood TPP in adult female lake trout fed alewives was one-third of that in controls fed a commercial diet. Adult lake trout from Lake Michigan had blood TPP levels similar to those of fish fed the alewife diet in the laboratory. Lake Superior lake trout had TPP levels similar to those of fish fed the control diet in the laboratory. Thiamine synthesis occurred in the intestine of lake trout. At least 81% of thiamine in the posterior intestine was synthesized, presumably by bacteria, when a <sup>14</sup>C-labeled thiamine diet was force-fed to lake trout. Thiamine had a long retention time in the lake trout: at 27 weeks after fish were injected with radioactive thiamine, blood cells retained 11% of the radioactivity that was present at 2 d and liver tissue retained 34% of the 2-d level. Lack of self-sustaining lake trout reproduction by Lake Michigan fish may be related to their lower blood thiamine levels. Thiamine deficiency may cause early mortality syndrome, which is common in Lake Michigan but not Lake Superior fish with higher blood thiamine levels.

Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea

1998 ◽  
Keyword(s):  
Great Lakes ◽  
Baltic Sea ◽  
Early Life ◽  
Lake Trout ◽  
Life Stage ◽  
Early Life Stage ◽  
Early Mortality ◽  
Thiamine Pyrophosphate ◽  
Critical Threshold ◽  
Early Mortality Syndrome

<em>Abstract</em>.—Dietary amprolium, a thiamine antagonist, was fed to lake trout <em>Salvelinus namaycush </em>broodstock from April to October before spawning to determine its effect on egg and tissue concentrations of thiamine, thiamine monophosphate, and thiamine pyrophosphate. The thiamine concentration of eggs from fish fed no amprolium was 61.8 nmol/g, whereas the concentration of thiamine in fish fed 0.05 and 0.10% amprolium was 4.02 and 1.71 nmol/g (<EM>P </EM>< 0.01), respectively. In lake trout fed 0.10% amprolium beginning in August, egg free thiamine concentration was reduced to 11.6 nmol/g. No sign of early mortality syndrome was observed in sac fry from eggs in this study, which suggests that thiamine concentrations in the egg were not low enough to be below a critical threshold or that factors other than thiamine are involved in early mortality syndrome.

Early Life Stage Mortality Syndrome in Fishes of the Great Lakes and Baltic Sea

1998 ◽  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Lake Trout ◽  
Life Stage ◽  
Lake Ontario ◽  
Early Life Stage ◽  
Fertilization Success ◽  
Thiamine Pyrophosphate ◽  
Predictive Index ◽  
Early Mortality Syndrome

<em>Abstract</em>.—Reproductive success and vitamin B<sub>1 </sub>(thiamine pyrophosphate, thiamine monophosphate, and free thiamine) concentrations were assessed in feral female lake trout <em>Salvelinus namaycush </em>from Lake Ontario and Lake Manitou. We monitored fertilization success, survival to hatch, incidence of blue-sac disease, other anomalies, and lake trout early mortality syndrome (EMS). Fertilization and hatching success were high, whereas mortality from blue-sac disease and other anomalies was low in egg batches from both lakes. There was no mortality from EMS in families from Lake Manitou. However, EMS occurred after hatching in the offspring of 48% of the females collected from Lake Ontario. We measured thiamine in liver, red blood cells, eggs, and developing embryos. Relative to fish collected in reference lakes, females in Lake Ontario had depressed hepatic, red blood cell, and egg thiamine concentrations. Although more extensive investigation of thiamine balance is required, it may be possible to use red blood cell thiamine pyrophosphate as a predictive index for EMS susceptibility in offspring. Total thiamine concentrations in developing embryos declined by 50% between fertilization and swim-up. Free thiamine reserves declined most rapidly, whereas levels of thiamine pyrophosphate increased between the eyed embryo and hatch stages. A high proportion (67%) of lake trout families in which the initial egg free thiamine reserves or embryonic concentrations of thiamine pyrophosphate levels were <0.8 nmol/g exhibited EMS. Below this threshold (0.8 nmol/g), the occurrence of EMS was variable (0–100%) and only weakly related to free thiamine concentrations (<em>r</em><sup>2</sup> = 0.32, <EM>P</EM> = 0.014). This observation implies the possibility of additional interactions with other factors.

Linking Egg Thiamine and Fatty Acid Concentrations of Lake Michigan Lake Trout with Early Life Stage Mortality

2009 ◽  
Vol 21 (4) ◽  
pp. 262-271 ◽  
Author(s):  
Sergiusz Czesny ◽  
John M. Dettmers ◽  
Jacques Rinchard ◽  
Konrad Dabrowski
Keyword(s):  
Fatty Acid ◽  
Early Life ◽  
Lake Michigan ◽  
Lake Trout ◽  
Life Stage ◽  
Early Life Stage

Residues of DDT in Lake Trout (Salvelinus namaycush) and Coho Salmon (Oncorhynchus kisutch) from the Great Lakes

1974 ◽  
Vol 31 (2) ◽  
pp. 191-199 ◽  
Author(s):  
Robert E. Reinert ◽  
Harold L. Bergman
Keyword(s):  
Lake Erie ◽  
Lake Michigan ◽  
Lake Trout ◽  
Coho Salmon ◽  
Marked Decrease ◽  
Life Stage ◽  
Oncorhynchus Kisutch ◽  
Salvelinus Namaycush ◽  
Spawning Season ◽  
Spawning Run

Concentrations of DDT residues were higher in lake trout (Salvelinus namaycush) from southern Lake Michigan in 1966–70 (average 18.1 ppm in fish 558–684 mm long) than in lake trout of the same size-class from Lake Superior in 1968–69 (4.4 ppm), and higher in adult coho salmon (Oncorhynchus kisutch) from Lake Michigan in 1968–71 (averages for different year-classes, 9.9–14.0 ppm) than in those from Lake Erie in 1969 (2.2 ppm). Residues were significantly higher in lake trout from southern Lake Michigan than in those from the northern part of the lake. In lakes Michigan and Superior, the levels increased with length of fish and percentage oil. In Lake Michigan coho salmon, the residues remained nearly stable (2–4 ppm) from September of the 1st yr of lake residence through May or early June of the 2nd yr, but increased three to four times in the next 3 mo. Residues in Lake Erie coho salmon did not increase during this period, which preceded the spawning season. Although the concentrations of total residues in whole, maturing Lake Michigan coho salmon remained unchanged from August 1968 until near the end of the spawning season in January 1969, the residues were redistributed in the tissues of the spawning-run fish; concentrations in the loin and brain were markedly higher in January than in August. This relocation of DDT residues accompanied a marked decrease in the percentage of oil in the fish, from 13.2 in August to 2.8 in January. Concentrations of residues were relatively high in eggs of both lake trout (4.6 ppm) and coho salmon (7.4–10.2 ppm) from Lake Michigan. The percentage composition of the residues (p,p′DDE, o,p′/DDT, p,p′DDT, and p,p′DDT) did not differ significantly with life stage, size, age, or locality, or date of collection of lake trout or coho salmon.

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