<p><strong>Objective:</strong> To explore the physiological and psychological nursing and nursing methods for infants during operation. <strong>Methods:</strong> The nursing measures are taken before, during and after operation for 138 infants that have received surgical treatment in our hospital from February 2013 to January 2015 after close observation. <strong>Results:</strong> The operations for all 138 infants have been completed successfully without any surgical complications. <strong>Conclusion: </strong>The sufficient pre-operative nursing for infants, the comfortable body position, suitable heat preservation and close observation during operation and the recovery from general anesthesia after operation are crucial to peri-operative nursing for infants.</p>