Evaluation the performance of sequencing batch reactor and bio-film sequencing batch reactor for pulp and paper wastewater treatment

2020 ◽  
Vol 208 ◽  
pp. 136-147
Author(s):  
Shrouk Adel ◽  
W.A. Ibrahim ◽  
Mai Fayed ◽  
Samia A. Aly
AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.


Vestnik MGSU ◽  
2019 ◽  
pp. 589-602 ◽  
Author(s):  
Tran Ha Quan ◽  
Elena S. Gogina

Introduction. Vietnamese urban municipal wastewater treatment plants are mainly of aeration-type facilities. Nowadays, an aeration-type plant, the Sequencing Batch Reactor (SBR), is widely applied and possesses a number of advantages over traditional systems with suspended activated sludge. Advantages of the SBR are mainly concluded in simplicity of operation, occupied area and cost. There is a number of problems at the wastewater treatment plants; they are connected with supplying only a half of wastewater design amount for the treatment as well as with quality of the purified water that must satisfy requirements of the Vietnamese discharge standard, the Standard A. Therefore, reconstruction and modification of the SBR is the major challenger to ensure the sustained development of large Vietnamese cities and maintenance of ecological balance. Materials and methods. To enhance the efficiency of wastewater purification in the SBR, the experiments were set on reactor reconstruction and modification by two directions: (1) Technological method, i.e. applying the Biochip 25 biocarrier, and (2) Operation method, i.e. adding the anoxic phase in reactor operation cycle. Laboratory tests were conducted for each of the directions, including comparison of a typical reactor with the modified one. Results. The study resulted in obtaining an optimal amount of the BioChip biocarrier material (10 to 20 %) that increased efficiency of wastewater purification by 10 to 20 %. In addition to this, when creating an anoxic phase of the operation cycle, efficiency of nitrogen removal increased by 20 %. When the denitrification occurs under the anoxic conditions, it contributes to stabilization of ammonium nitrogen removal for daily nitrogen loading in reactor of 0.3 to 0.8 TKN kg/sludge kg. Conclusions. The suggested technology provides the quality of treated water corresponding with the Vietnamese Standard A requirements. At the present, it is planned to proceed with the experiment on the base of Vietnamese semi-industrial plant for research and appraisal of the SBR reconstruction and modification method. Acknowledgements. The authors are grateful to AKVA Control company in Samara for granted biocarrier Mutag BioChip 25 and to Associate Professor Tran Van Quang and his students, Nguyen Ngoc Phuong and Truong Quoc Dai, of Environment Protect Research Center, Danang University for support of the experiment.


2019 ◽  
Vol 38 (3) ◽  
pp. 243
Author(s):  
Happy Mulyani ◽  
Gregorius Prima Indra Budianto ◽  
Margono Margono ◽  
Mujtahid Kaavessina

Industrial wastewater treatment using Sequencing Batch Reactor (SBR) can improve effluent quality at lower cost than that obtained by other biological treatment methods. Further optimization is still required to enhance effluent quality until it meets standard quality and to reduce the operating cost of treatment of high strength organic wastewater. The purpose of this research was to determine the effect of pretreatment (pH adjustment and prechlorination) and aeration time on effluent quality and COD removal rate in tapioca wastewater treatment using SBR. Pretreatment was done by (1) adjustment of tapioca wastewater pH to control (4.92), 7, and 8, and (2) tapioca wastewater prechlorination at pH 8 during hour using calcium hypochlorite in variation dosages 0, 2, 4, 6 mg/L Cl2, SBR operation was conducted according to following steps: (1) Filling of pre-treated wastewater into a bioreactor during 1 hour, and (2) aeration of the mixture of tapioca wastewater and activated sludge during 8 hours. Effluent sample was collected at every 2-hours aeration for COD analysis. COD removal rate mathematical formula was got by first deriving the best fit function between aeration time and COD. Optimum aeration time resulting in no COD removal rate. The value of COD effluent and its removal rate in optimum aeration time was used to determine the recommended of operation condition of pretreatment. Research result shows that chosen pH operation condition is pH 8. Prechorination can make effluent quality which meets standard quality and highest COD removal rate. The chosen Cl2 dosage is 6 mg/L.


2006 ◽  
Vol 54 (11-12) ◽  
pp. 241-248 ◽  
Author(s):  
J. Simon ◽  
J. Wiese ◽  
H. Steinmetz

Integrated operation strategies for combined sewer and WWTP are becoming more and more popular because of numerous benefits. One can find many examples in literature, but many of these studies deal with partly fictitious systems and/or were accomplished within the planning. Hence most of these studies do not have to deal with the restrictions given by already built constructions. The authors, who worked on several integrated projects, will discuss the requirements of SBR and CFR plants concerning an implementation of integrated operation.


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