scholarly journals Thickness of the epithelium and the inflammatory cell infiltrate in oral lichen planus. A morphometric study

2014 ◽  
Vol 6 (2) ◽  
pp. 78-82
Author(s):  
D.B Nandini ◽  
VV Ramya
Keyword(s):  
Image Analysis ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Cell ◽  
Morphometric Study ◽  
Inflammatory Cell Infiltrate ◽  
Analysis Software ◽  
Cell Infiltrate ◽  
Image Analysis Software ◽  
Oral Lichen

Abstract Background Oral lichen planus is a chronic mucocutaneous disease of uncertain etiology. Inflammatory cell infiltrate plays an important role in pathogenesis of oral lichen planus. It is said that hyperplastic epithelium is seen where the inflammatory cell infiltrate is mild while beneath atrophic epithelium dense inflammatory cell infiltrate is evident. Studies have shown negative correlation between thickness of the epithelium and thickness of inflammatory cell infiltrate. Morphometric studies in oral lichen planus are very scanty and have been performed using stage micrometer and eye piece graticule. Using Image Analysis Software can avoid inter-observer variations which has not been done till date. Aim and Objectives The present study aimed at evaluating thickness of epithelium and thickness of inflammatory cell infiltrate and to determine any existing relation between the same using Image Analysis Software. Other histopathologic features were also evaluated. Materials and method 58 confirmed cases of oral lichen planus from buccal mucosa were retrieved from department archives. 6μ thick sections were stained with Hematoxylin and Eosin. Six non-overlapping fields were selected randomly from each section and photomicrographs were captured under 4x objective using Trinocular Research Microscope. Morphometry was done using Image Analysis Software and data was stored in Microsoft excel for statistical analysis. Results In present study the mean of epithelium thickness was 281.2059 and inflammatory cell infiltrate thickness was 330.2540. An inverse correlation was observed. As the thickness of inflammatory cell infiltrate increased there was a decrease in thickness of epithelium (Coefficient -0.156). Conclusion Thickness of the epithelium may vary according to the site, however in this study all cases were from buccal mucosa. Inflammatory cell infiltrate influences overlying thickness of epithelium and determines its nature. How to cite this article Ramya VV, Nandini DB, Praveen SB, Madhushankari GS. Thickness of the epithelium and the inflammatory cell infiltrate in oral lichen planus. A morphometric study. CODS J Dent 2014;6;78-82

scholarly journals Higher Number of EBI3 Cells in Mucosal Chronic Hyperplastic Candidiasis May Serve to Regulate IL-17-Producing Cells

2021 ◽  
Vol 7 (7) ◽  
pp. 533
Author(s):  
Ailish Williams ◽  
Helen Rogers ◽  
David Williams ◽  
Xiao-Qing Wei ◽  
Damian Farnell ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Inflammatory Cell ◽  
Candida Infection ◽  
Inflammatory Cell Infiltrate ◽  
T Helper ◽  
Cell Infiltrate ◽  
High Prevalence ◽  
Squamous Cell Papilloma ◽  
Oral Lichen

Previous research into the inflammatory cell infiltrate of chronic hyperplastic candidosis (CHC) determined that the immune response is primarily composed of T cells, the majority of which are T helper (CD4+) cells. This present investigation used immunohistochemistry to further delineate the inflammatory cell infiltrate in CHC. Cells profiled were those expressing IL-17A cytokine, EBI3 and IL-12A subunits of the IL-35 cytokine, and FoxP3+ cells. Squamous cell papilloma (with Candida infection) and oral lichen planus tissues served as comparative controls to understand the local immune responses to Candida infection. The results demonstrated that Candida-induced inflammation and immune regulation co-exist in the oral mucosa of CHC and that high prevalence of cells expressing the EBI3 cytokine subunit may play an important role in this regulation. This balance between inflammation and immune tolerance toward invading Candida in the oral mucosa may be critical in determining progress of infection.

scholarly journals A comparative study of apoptosis in reticular and erosive oral lichen planus

2012 ◽  
Vol 23 (5) ◽  
pp. 564-569 ◽  
Author(s):  
Juliana M. C. Brant ◽  
Maria Cássia F. Aguiar ◽  
Helenice A. M. Grandinetti ◽  
Luciana V. Rodrigues ◽  
Anilton C. Vasconcelos
Keyword(s):  
Lichen Planus ◽  
Inflammatory Disease ◽  
Oral Lichen Planus ◽  
Inflammatory Cell ◽  
Chronic Inflammatory Disease ◽  
Clinical Presentations ◽  
Tunel Reaction ◽  
Lymphocytic Infiltrates ◽  
Inflammatory Infiltrates ◽  
Oral Lichen

The oral lichen planus (OLP) is a chronic inflammatory disease, probably autoimmune, with different clinical forms. The most common types are the reticular and the erosive ones. Apoptosis participates in the destruction of basal keratinocytes, but its role in the perpetuation of the subepithelial lymphocytic infiltrates was not yet investigated. To evaluate the involvement of apoptosis in the epithelium and in subepithelial lymphocytic infiltrates, 15 samples of reticular and erosive OLP and 10 samples of healthy oral mucosa were collected and processed histologically. Apoptosis was quantified in the epithelium and in inflammatory cell infiltrates. TUNEL reaction was used to measure apoptosis in the infiltrates. Erosive OLP showed more intense epithelial apoptosis than reticular OLP and controls. In contrast, apoptosis in the inflammatory cell infiltrates was more frequent in reticular than in erosive OLP. Lymphocytes were the predominant cells within the inflammatory cell infiltrates and were more frequent in erosive OLP than in reticular type. These results suggest that different apoptotic levels are involved in the erosive/reticular switch in OLP, determining different clinical presentations. In conclusion, decreased apoptosis in inflammatory infiltrates may contribute to the persistence of T lymphocytes, worsening the attack to the epithelium in erosive OLP.

scholarly journals Oral lichen planus: a clinical and morphometric study of oral lesions in relation to clinical presentation

2004 ◽  
Vol 15 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Juan Seoane ◽  
María Amparo Romero ◽  
Pablo Varela-Centelles ◽  
Pedro Diz-Dios ◽  
María José Garcia-Pola
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Clinical Presentation ◽  
Chronic Inflammatory Disease ◽  
Plasma Cortisol Level ◽  
Biological Behavior ◽  
Morphometric Study ◽  
Chi Square ◽  
Clinical Presentations ◽  
Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical presentations that can be classified as reticular or atrophic-erosive. Sixty-two OLP patients were studied to evaluate the clinical-pathologic characteristics of their OLP lesions and to investigate possible differences in their biological behavior. The most common clinical presentation was the reticular type (62.9% vs 37.1%). Atrophic-erosive presentations showed significantly longer evolution (chi square=4.454; p=0.049), more extensive lesions (chi square=16.211; p=0.000) and more sites affected than reticular ones (chi square=10.048; p=0.002). Atrophic-erosive OLP was more frequently found on the tongue, gingiva and floor of the mouth. No statistically significant differences could be identified between reticular and atrophic-erosive clinical presentations in terms of age, sex, tobacco habit, plasma cortisol level and depth of inflammatory infiltrate. We concluded that the classification of OLP lesions as reticular vs atrophic-erosive is a simple, easy to use classification that can identify clinical presentations with different biological behavior.

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