scholarly journals Evaluation of Hemocompatibility of Titanium after Various Surface Treatments: An in vitro Study

Author(s):  
Yemineni BhavanChand ◽  
R Ranzani ◽  
H Annapoorani

ABSTRACT Objective To evaluate the hemocompatibility of titanium after various surface treatments. Materials and methods A total of 27 disk-shaped specimens (3 ⨯ 10.0 mm) were prepared from a cylindrical rod of medical grade titanium. The disks were divided into three groups, of which one was considered as the control (mechanical surface polished surface). The other groups being sandblasted disks and anodized disks. Surface evaluation was done for sandblasted and anodized disks with scanning electron microscope. The specimens were placed in polystyrene culture plates and agitated with phosphate buffered saline for 5 minutes before they were exposed to blood taken from human volunteer. The materials were under 30 minutes agitation at 75 ± 5 rpm using an Environ shaker thermostated at 35 ± 20°C. The total hemoglobin from the initial sample was measured using automatic hematology analyzer. Percentage hemolysis, thrombin time, platelet adhesion and activation were assessed. Significant differences between different treated titanium materials were determined using Minitab® Version 15.1.1.0. A two-sample t-test was performed to find the p-values for different groups of data. Results After 30 minutes of agitation, cells began to spread on the test surfaces. There was a clear reduction in the number of platelets before and after exposure to titanium samples. Reduction of leukocytes was seen to a least extent on the anodized surface. Rough surface induced higher hemolysis than other groups. Platelet reduction and leukocyte reduction in all the three surfaces was quite higher than that obtained for reference plate. Surface variation has no significance on the thrombogenic capabilities of medical grade titanium (p < 0.05). Significance The hemocompatibility of medical grade titanium did not vary with different surface modifications. How to cite this article BhavanChand Y, Ranzani R, Annapoorani H. Evaluation of Hemocompatibility of Titanium after Various Surface Treatments: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):136-142.

Author(s):  
Sílvia Fontes Do Amaral Pereira

O objetivo do presente estudo in vitro foi verificar, através de teste de cisalhamento, a resistência de união da liga Wironia®light, à base de níquel-cromo sem berílio, sujeita a diferentes tratamentos de superfície, às cerâmicas Vita VM13 e Noritake. Foram confeccionados oitenta espécimes cilíndricos metálicos, com o auxílio de uma matriz de aço, nas dimensões de 4 mm de diâmetro por 4 mm de altura. Os espécimes foram divididos em oito grupos (n=10), de acordo com o tipo de tratamento superficial aplicado à liga metálica e com o tipo de cerâmica testada. Estes foram avaliados de acordo com os critérios de resistência ao cisalhamento e, com o auxílio de microscopia óptica, foi avaliada a área de cerâmica remanescente aderida ao metal após a fratura. Os corpos-de-prova do G6 (fabricados em Cerâmica Noritake com jateamento-26,401 ± 11,637 MPa) apresentaram maior resistência ao cisalhamento (p> 0,05) enquanto que os menores valores foram registrados no G4 (Cerâmica Vita com utilização de broca-13,440 ± 7,766 MPa). G6 (19425,4 μm2) apresentou a maior área de cerâmica aderida ao metal (p> 0,05) enquanto que o G4 (2310,2 μm2) apresentou a menor área. Concluiu-se que G6 obteve os valores mais altos de resistência ao cisalhamento e de remanescente cerâmico aderido à superfície metálica enquanto que o G4 obteve os valores mais baixos. Descritores: Restaurações metalo-cerâmicas; ligas de níquel-cromo;porcelana dentária.


2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Erica Scalambrino ◽  
Lidia Padovan ◽  
Veena Chantarangkul ◽  
Marigrazia Clerici ◽  
Andrea Artoni ◽  
...  

2020 ◽  
Vol 11 (2) ◽  
pp. 38
Author(s):  
Minh N. Luong ◽  
Laurie Huang ◽  
Daniel C. N. Chan ◽  
Alireza Sadr

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.


2016 ◽  
Vol 20 (8) ◽  
pp. 2323-2323
Author(s):  
Sebastian Zingler ◽  
Andreas Sommer ◽  
Sinan Sen ◽  
Daniel Saure ◽  
Jochen Langer ◽  
...  

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