scholarly journals In Vitro Study on the Effect of a New Bioactive Desensitizer on Dentin Tubule Sealing and Bonding

2020 ◽  
Vol 11 (2) ◽  
pp. 38
Author(s):  
Minh N. Luong ◽  
Laurie Huang ◽  
Daniel C. N. Chan ◽  
Alireza Sadr

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.

2019 ◽  
Vol 13 (4) ◽  
pp. 305-310
Author(s):  
Mina Biria ◽  
Sajedeh Namaei Ghasemi ◽  
Seyedeh Mahsa Sheikh-Al-Eslamian ◽  
Narges Panahandeh

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.


Author(s):  
Masoud Fallahinejad Ghajari ◽  
Amir Ghasemi ◽  
Mohammadreza Badiee ◽  
Zahra Abdolazimi ◽  
Alireza Akbarzadeh Baghban

Objectives: This study aimed to assess the microshear bond strength (MSBS) of Scotchbond Universal adhesive, used in self-etch and etch-and-rinse modes, to primary and permanent dentin at 24 hours and six months. Materials and Methods: A total of 88 composite micro-cylinders were divided into eight groups (n=11) as follows: (A) Etch-and-rinse, 24 hours, primary dentin; (B) Self-etch, 24 hours, primary dentin; (C) Etch-and-rinse, six months, primary dentin; (D) Self-etch, six months, primary dentin; (E) Etch-and-rinse, 24 hours, permanent dentin; (F) Self-etch, 24 hours, permanent dentin; (G) Etch-and-rinse, six months, permanent dentin; (H) Self-etch, six months, permanent dentin. The MSBS was measured by a testing machine at a crosshead speed of 0.5 mm/minute. Data were analyzed using three-way analysis of variance (ANOVA). Results: The mean MSBS was 12.3±2.3 MPa in A, 18.8±4.1 MPa in B, 11.9±3.7 MPa in C, 16±2.9 MPa in D, 19.1±2.7 MPa in E, 22.8±4.1 MPa in F, 16.2±2.6 MPa in G, and 17.2±4.4 MPa in H. In the self-etch mode, the MSBS was significantly higher than that in the etch-and-rinse mode (P<0.001). The MSBS in permanent teeth was significantly higher than primary teeth (P<0.001). At six months, the MSBS significantly decreased in all groups (P<0.001). Conclusion: The micro-shear bond strength of Scotchbond Universal adhesive decreases over time and depends on the type of tooth and the mode of application of the adhesive.


2021 ◽  
Vol 11 (4) ◽  
pp. 306-312
Author(s):  
Dalia M. Abouelmagd ◽  
Hanan K. Abouelseoud

Objectives: This study aimed to compare the microshear bond strength (µSBS) of self-adhesive flowable composite and conventional bonded flowable composite to sound and caries affected dentin. Materials and Methods: In this in vitro study, a total of freshly extracted 40 human molar teeth were used. The occlusal surface was sectioned to expose dentin for µSBS test. The teeth were randomly divided into 2 groups according to the type of flowable composite used into two groups (n=20): group A: self- adhesive flowable composite (Vertise flow), group B: Filtek Z350-XT flowable composite and one-step self-etch adhesive Adper Easy One. A plastic cylindrical mold was used to construct resin composite cylinders (0.8mm diameter x 1mm length) on the sound and caries affected exposed dentin and filled with flowable composite restorative materials according to manufacturer’s instructions. The samples were then immersed in distilled water at 37°C for 24 hours, and underwent µSBS test at a crosshead speed of 0.5 mm/min. Data were analyzed using Student's t-test to compare between the two materials as well as to compare between sound and caries affected dentin. Results: The mean values of µSBS of Filtek Z-350XT to sound and caries affected were 26.6 and 26.7 MPa respectively. The mean µSBS of Vertise Flow to sound and affected were 13.2 and 17.9 MPa respectively. With sound dentin FiltekZ-350XT showed statistically significant higher µSBS than Vertise flow. With caries affected dentin, there was no statistically significant difference between the two materials. There was no statistically significant difference between µSBS of sound and caries affected dentin within each material. The significance level was set at P ≤ 0.05. Conclusions: Within the limitations of this study, the conventional flowable composite yielded the highest µSBS to sound dentin. There was no statistical significant difference in µSBS of self-adhesive and conventional flowable to caries affected dentin. Key words: Composite Resins; Dentin; microshear.


2021 ◽  
Vol 24 (4 suppl 1) ◽  
Author(s):  
Rubens Nazareno GARCIA ◽  
Vitaliano Gomes de ARAÚJO ◽  
Camila Ribeiro SILVA ◽  
Luiz Carlos Machado MIGUEL ◽  
Marcelo GIANNINI

2021 ◽  
Vol 13 (4) ◽  
pp. 237
Author(s):  
Katherine Joselyn Atoche-Socola ◽  
Luis Ernesto Arriola-Guillén ◽  
Ana Isabel López-Flores ◽  
Isadora Martini Garcia ◽  
Gustavo Huertas-Mogollón ◽  
...  

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