Determining of Erythrocyte Sedimentation Rate (ESR) and Packed Cell Volume (PCV)

Author(s):  
Aarti Mahajan
Blood ◽  
1953 ◽  
Vol 8 (10) ◽  
pp. 893-904 ◽  
Author(s):  
ALDULA J. MEYERS ◽  
VIRGINIA TREVORROW ◽  
ALFRED H. WASHBURN ◽  
EDWARD R. MUGRAGE

Abstract The failure to devise a satisfactory means of adjusting all sedimentation rates to a standard cell volume, together with a critical analysis of the results of other investigators, casts serious doubt on the validity of any such correction charts. Two formulae are presented by means of which the sedimentation rate of a given sample of blood can be predicted on the basis of the plasma/cell ratio and plasma proteins concentrations. One formula utilizes the results of saltingout analysis of plasma proteins, the other those of electrophoretic analysis of serum proteins. The most influential factors in determininsg sedimentation rate are plasma/cell ratio, fibrinogen, alpha-2 globulin, and gamma globulin. The results suggest that the effective concentrations of the plasma proteins are most adequately expressed as concentrations per unit of cell volume. Addition of purified protein fractions (fibrinogen, gamma globulin, and albumin) to the blood in vitro confirm the findings from the statistical studies and suggest a direct cause and effect relationship between the concentration of these proteins and the rate of erythrocyte sedimentation. Determination of the erythrocyte sedimentation rate cannot give, per se, any clue as to the level of any one of the responsible factors. An appreciation of the multiple factors involved is essential for an adequate interpretation of the clinical significance of a sedimentation rate determination.


2012 ◽  
Vol 3 (1) ◽  
pp. 17-19
Author(s):  
MO Faruqe ◽  
MR Alam ◽  
MER Bhuiyan ◽  
M Myenuddin

To evaluate the certain haematobiochemical parameters in different ages of Hubbard Classic broiler birds, the study was performed at Kazi Farms Ltd., Gazipur in collaboration with the Department of Physiology, Bangladesh Agricultural University, Mymensingh for a period of one month (1st to 30th March 2002). On haematobiochemical examination, total erythrocyte count, haemoglobin content, packed cell volume, clotting time, glucose and uric acid concentration were gradually increased with advancement of age of birds. The values of TEC, Hb, PCV, clotting time, glucose and uric acid were recorded at day old birds as average 1.23 ± 0.02 million / cmm, 3.90 ± 0.09 g%, 19.60± 0.24 % , 37.20 ± 1.50 second, 179.20 ± 8.95 mg / dl and 3.72 ± 0.58 mg / dl respectively while the values at day 30 were 2.43 ± 0.07 million / cmm, 7.26 ± 0.16 g%, 28.20 ± 0.58%, 206.40 ± 10.17 second, 271.60 ± 8.56 mg / dl and 6.42 ± 0.65 mg / dl respectively which were found statistically significant (p < 0.01). On the other hand erythrocyte sedimentation rate, cholesterol and urea concentration were gradually decreased where significantly (p < 0.05) higher values (2.80 ± 0.20 mm in 1st hour, 330.40 ± 38.52 mg / dl and 26.42 ± 2.06 mg/ dl respectively) were recorded at day old chicks and lower values (1.80 ± 0.37 mm in 1st hour, 104.20 ± 3.24 mg / dl and 17.00 ± 0.07 mg / dl respectively) were recorded at age of 30 days old of birds. The findings of the present work clearly indicate that age has got influential effect on the body haemopoietic system of the broiler birds as well as haematobiochemical values.


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