<p>ABSTRACT<br />Objective: To find the stigmasterol source of Artemisia annua L. and to study the profile of phytosterol types in A. annua hairy root cultures.<br />Methods: Stigmasterol content determination was done based on stigmasterol content in the methylene chloride extract of A. annua using high<br />performance liquid chromatography in wild-type plant, plant tissue cultures, and hairy root cultures. The optimal resolution was achieved by<br />suitability system, and stigmasterol calibration curve was generated with stigmasterol concentrations series. Phytosterol types in A. annua hairy root<br />cultures were performed by gas chromatography-mass spectrophotometry.<br />Results: Highest stigmasterol content in A. annua is shown by hairy root cultures of A. annua in the 5<br /> week harvesting time, which is 1.5 g/100 g (w/w)<br />extract. The phytosterol profile in A. annua hairy root cultures encompassed stigmasterol 74.6%, β-sitosterol 13.5%, and campesterol 11.9%.<br />th<br />Conclusion: A. annua hairy root cultures in 5-week harvesting time prove its potential as stigmasterol source alternative. The highest phytosterol type<br />in A. annua hairy root cultures is stigmasterol. This is the first report stigmasterol content and its phytosterol profile of A. annua hairy root cultures<br />extract.<br />Keywords: Artemisia annua L., Wild-type plant, Plant tissue cultures, Hairy root cultures, Stigmasterol.</p>
Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of Artemisia annua L.
