scholarly journals Hydrogen Exchange Study of DNA Duplexes Containing the Consensus Binding Site for Arabidopsis thaliana SPL14 Transcription Factor

2013 ◽  
Vol 34 (8) ◽  
pp. 2511-2514 ◽  
Author(s):  
Minjee Jeong ◽  
Jin-Wan Park ◽  
Hee-Eun Kim ◽  
Ju-Yong Lee ◽  
Ae-Ree Lee ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Binding Site ◽  
Hydrogen Exchange ◽  
Dna Duplexes ◽  
Consensus Binding Site ◽  
Exchange Study

Prediction and experimental validation of a putative non-consensus binding site for transcription factor STAT3 in serum amyloid A gene promoter

2013 ◽  
Vol 1830 (6) ◽  
pp. 3650-3655 ◽  
Author(s):  
Prabha Tiwari ◽  
Lokesh P. Tripathi ◽  
Teppei Nishikawa-Matsumura ◽  
Shandar Ahmad ◽  
Soken-Nakazawa J. Song ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Binding Site ◽  
Experimental Validation ◽  
Serum Amyloid A ◽  
Gene Promoter ◽  
Serum Amyloid ◽  
Consensus Binding Site ◽  
Amyloid A

ZFP143 Activates C/EBPα Transcription in Myeloid Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1233-1233
Author(s):  
Boris Bartholdy ◽  
David Gonzalez ◽  
Daniel G. Tenen
Keyword(s):  
Transcription Factor ◽  
Binding Site ◽  
Blast Crisis ◽  
Myeloid Cells ◽  
Gene Trap ◽  
Myelogenous Leukemia ◽  
Mrna Levels ◽  
Consensus Binding Site ◽  
Differentiation Block

Abstract C/EBPα is a key transcription factor involved in myeloid differentiation and frequently mutated or deregulated in human acute myeloid leukemias (AML) as well as in blast crisis of chronic myelogenous leukemia (CML). Disruption of its function contributes to the differentiation block observed in these diseases and thus to leukemogenesis. Here, we have identified a conserved region in the C/EBPα promoter that is important for activation of C/EBPα transcription in myeloid cells and narrowed it down to a conserved site of approximately 25 bp that contains a consensus binding site for ZFP143, a seven-zinc finger transcription factor. In gel retardation assays, this region bound a factor of approximately 100 kDa that we biochemically purified and identified by mass spectrometry as being indeed ZFP143. In vivo binding of ZFP143 to its bona fide binding site in the C/EBPα promoter was also detected by chromatin immunoprecipitation assays. We are now studying the in vivo effect of ZFP143 on C/EBPα transcription in mice that carry an inducible gene trap in the ZFP143 locus. While mice homozygous for the active gene trap - that prevents ZFP143 transcription - die at an early embryonic stage, preliminary data from heterozygous mice shows indeed a reduction of C/EBPα mRNA levels, suggesting a role for ZFP143 in C/EBPα transcription activation. Ongoing experiments aim at selectively inactivating ZFP143 in the hematopoietic system using the Mx1-Cre / loxP system.

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