scholarly journals Impact of adipogenic differentiation on stemness and osteogenic gene expression in extensive culture of human adipose-derived stem cells

2014 ◽  
Vol 3 ◽  
pp. 597-606 ◽  
Author(s):  
Wan Kamarul Zaman Wan Safwani ◽  
Suzana Makpol ◽  
Somasundaram Sathapan ◽  
Kienhui Chua
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Adipogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals MicroRNA treatment modulates osteogenic differentiation potential of mesenchymal stem cells derived from human chorion and placenta

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kulisara Marupanthorn ◽  
Chairat Tantrawatpan ◽  
Pakpoom Kheolamai ◽  
Duangrat Tantikanlayaporn ◽  
Sirikul Manochantr
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factor ◽  
Osteogenic Differentiation ◽  
Differentiation Potential ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

AbstractMesenchymal stem cells (MSCs) are important in regenerative medicine because of their potential for multi-differentiation. Bone marrow, chorion and placenta have all been suggested as potential sources for clinical application. However, the osteogenic differentiation potential of MSCs derived from chorion or placenta is not very efficient. Bone morphogenetic protein-2 (BMP-2) plays an important role in bone development. Its effect on osteogenic augmentation has been addressed in several studies. Recent studies have also shown a relationship between miRNAs and osteogenesis. We hypothesized that miRNAs targeted to Runt-related transcription factor 2 (Runx-2), a major transcription factor of osteogenesis, are responsible for regulating the differentiation of MSCs into osteoblasts. This study examines the effect of BMP-2 on the osteogenic differentiation of MSCs isolated from chorion and placenta in comparison to bone marrow-derived MSCs and investigates the role of miRNAs in the osteogenic differentiation of MSCs from these sources. MSCs were isolated from human bone marrow, chorion and placenta. The osteogenic differentiation potential after BMP-2 treatment was examined using ALP staining, ALP activity assay, and osteogenic gene expression. Candidate miRNAs were selected and their expression levels during osteoblastic differentiation were examined using real-time RT-PCR. The role of these miRNAs in osteogenesis was investigated by transfection with specific miRNA inhibitors. The level of osteogenic differentiation was monitored after anti-miRNA treatment. MSCs isolated from chorion and placenta exhibited self-renewal capacity and multi-lineage differentiation potential similar to MSCs isolated from bone marrow. BMP-2 treated MSCs showed higher ALP levels and osteogenic gene expression compared to untreated MSCs. All investigated miRNAs (miR-31, miR-106a and miR148) were consistently downregulated during the process of osteogenic differentiation. After treatment with miRNA inhibitors, ALP activity and osteogenic gene expression increased over the time of osteogenic differentiation. BMP-2 has a positive effect on osteogenic differentiation of chorion- and placenta-derived MSCs. The inhibition of specific miRNAs enhanced the osteogenic differentiation capacity of various MSCs in culture and this strategy might be used to promote bone regeneration. However, further in vivo experiments are required to assess the validity of this approach.

scholarly journals Monocyte Exosomes Stimulate the Osteogenic Gene Expression of Mesenchymal Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75227 ◽  
Author(s):  
Karin Ekström ◽  
Omar Omar ◽  
Cecilia Granéli ◽  
Xiaoqin Wang ◽  
Forugh Vazirisani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

Stimulators of osteogenic gene expression in human mesenchymal stem cells into osteoblasts in vitro

Bone ◽  
2012 ◽  
Vol 50 ◽  
pp. S164
Author(s):  
T. Kim⁎ ◽  
S. Lee ◽  
H.-Y. Chung ◽  
D.-W. Byun ◽  
M.-I. Kang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals Bisphosphonates induce the osteogenic gene expression in co‐cultured human endothelial and mesenchymal stem cells

2013 ◽  
Vol 18 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Viviana Ribeiro ◽  
Mónica Garcia ◽  
Raquel Oliveira ◽  
Pedro S. Gomes ◽  
Bruno Colaço ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals 5-Azacytidine facilitates osteogenic gene expression and differentiation of mesenchymal stem cells by alteration in DNA methylation

2009 ◽  
Vol 60 (1-3) ◽  
pp. 11-22 ◽  
Author(s):  
Guo-Shun Zhou ◽  
Xiao-Lei Zhang ◽  
Jun-Ping Wu ◽  
Rui-Peng Zhang ◽  
Li-Xin Xiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals EphrinB2 regulates osteogenic differentiation of periodontal ligament stem cells and alveolar bone defect regeneration in beagles

2019 ◽  
Vol 10 ◽  
pp. 204173141989436 ◽  
Author(s):  
Penglai Wang ◽  
Wen Wang ◽  
Tengyu Geng ◽  
Yi Liu ◽  
Shaoyue Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Bone Homeostasis ◽  
Periodontal Ligament Stem Cells ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

EphrinB2, a membrane protein regulating bone homeostasis, has been demonstrated to induce osteogenic gene expression in periodontal ligament fibroblasts. The aim of this study was to explore the effects of ephrinB2 on osteogenic differentiation of periodontal ligament stem cells and on alveolar bone regeneration in vivo. We assessed the osteogenic gene expression and osteogenic differentiation potential of ephrinB2-modified human and canine periodontal ligament stem cells, in which ephrinB2 expression was upregulated via lentiviral vector transduction. EphrinB2-modified canine periodontal ligament stem cells combined with PuraMatrix were delivered to critical-sized alveolar bone defects in beagles to evaluate bone regeneration. Results showed that ephrinB2 overexpression enhanced osteogenic gene transcription and mineral deposition in both human and canine periodontal ligament stem cells. Animal experiments confirmed that ephrinB2-modified canine periodontal ligament stem cells + PuraMatrix resulted in greater trabecular bone volume per tissue volume and trabecular thickness compared with other groups. Our study demonstrated that ephrinB2 promoted osteogenic differentiation of periodontal ligament stem cells and alveolar bone repair in beagles, highlighting its therapeutic potential for the treatment of alveolar bone damage.

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