Gingival crevicular fluid levels of sclerostin in chronic periodontitis and healthy subjects

Studies on the link between periodontal disease and atherosclerosis have generated conflicting results and the mechanisms underlying this relationship are incompletely understood. Therefore, this study aimed to assess the levels in serum and in gingival crevicular fluid (GCF) of TNF-a, IL-1b and IL-6, to clarify the possible link between periodontitis and hyperlipidemia, as well as the effects of conventional periodontal treatment through scaling and root surfacing on these pro-inflammatory molecules. The study was carried out on a total of 40 subjects divided into two main groups: the study group (n=26) and control group (n=14). The cases included patients with atherosclerosis with prescribed diet (D) or antilipemic therapy with a drug from the statin group (S). Controls (C) were selected from systemically healthy subjects with chronic periodontitis. Samples were performed from crevicular fluid and serum, by determining the initial and post-treatment of TNF-a, IL-1b and IL-6. For all groups there were significant serum decreases in TNF-a, IL-1b and IL-6 from baseline, and the decreases were more significant IL-1b for statin group. Significant decreases were found in the crevicular fluid for all cytokines. The decrease was most evident for IL-6 in the statin group. Combining antilipemic periodontal therapy and treatment can provide beneficial effects on metabolism and control of inflammatory atherosclerosis by lowering serum pro-inflammatory cytokines.

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Rapid Method for the Detection of Porphyromonas gingivalis in Chronic Periodontitis

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2019.2923 ◽

2019 ◽

Vol 11 (5) ◽

pp. 689-695

Author(s):

Jia Yuan ◽

Qiongyu Chen ◽

Xiongjun Xu

Keyword(s):

Real Time ◽

Rapid Method ◽

Porphyromonas Gingivalis ◽

Real Time Pcr ◽

Chronic Periodontitis ◽

Healthy Subjects ◽

Gingival Crevicular Fluid ◽

Magnetic Microbeads ◽

Health Complications ◽

Crevicular Fluid

Porphyromonas gingivalis is the major cause of chronic periodontitis, a disease leading to the loss of teeth and other health complications. Therefore, an urgent need exists for a specific and rapid method for the detection of this pathogen in affected patients. The objective of this study was to test the applicability of conventional and real-time PCR protocols to detect the presence of P. gingivalis using DNA isolated by magnetic microbeads. The samples were collected from 50 patients with periodontal disease and 50 healthy subjects. Following successful isolation of DNA, the presence of P. gingivalis gene coding for its 16S rRNA was established by conventional PCR or quantitative realtime PCR. The bacteria were identified in 94% of the patients when samples of gingival crevicular fluid obtained from the active disease zone were used. The corresponding value for the quiescent zone was 44%, and for the samples collected from the plaque was 12%. P. gingivaliswas not found in samples obtained from healthy subjects. Thus, the methodology developed here, based on isolation of DNA from affected periodontium by magnetic microbeads and detection of P. gingivalis DNA by conventional or quantitative real-time PCR, has been proven to be specific, sensitive, and accurate. It provides a valuable tool for a rapid and reliable diagnosis of an imminent or ongoing disease.

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