scholarly journals Function of oxygen transport in the blood of patients in a state of alcohol deprivation in blood in vitro incubation with ethanol solution

2021 ◽  
Vol 18 (4) ◽  
pp. 108-113
Author(s):  
H. V. Lelevich
Keyword(s):  
Oxygen Transport ◽  
Ethanol Solution ◽  
Final Concentration ◽  
Acid Base ◽  
Blood Samples ◽  
Healthy Donors ◽  
Chronic Ethanol Consumption ◽  
Acid Base Status ◽  
Male Patients

Objective. To estimate the in vitro effect of ethanol on the function of oxygen transport in the blood of patients in a state of alcohol deprivation and in healthy donors.Materials and methods. We analyzed blood samples from 13 male patients aged 22–56 in a state of alcohol deprivation and 11 males aged 20-45 in whom this diagnosis was excluded. The blood was incubated with ethanol solution at a final concentration of 50 μmol/L. The parameters of the function of oxygen transport (р50, рО2, рСО2, рН, АВЕ, НСО3-, ТСО2,SВЕ, SBC) of the blood taken from the cubital vein using an ABL-330 “Radiometr” microgas analyzer were determined.Results. The patients in a state of alcohol deprivation reveal increased affinity of hemoglobin for oxygen, and their acid-base status of the blood becomes alkaline. When ethanol is added in vitro to the blood of patients, the affinity of hemoglobin for oxygen decreases.Conclusion. Decreased affinity of hemoglobin for oxygen upon the in vitro addition of ethanol in the examined patients indicates compensatory and adaptive changes in the oxygen transport of the blood in response to chronic ethanol consumption.

Intraosseous Blood Samples Are Predictive for Central Venous Acid-Base Status and Oxygenation

2002 ◽  
Vol 96 (Sup 2) ◽  
pp. A377
Author(s):  
Joerg Kampen ◽  
Peter H. Tonner ◽  
Juergen Gernhuber ◽  
Berthold Bein ◽  
Jens Scholz
Keyword(s):  
Central Venous ◽  
Acid Base ◽  
Blood Samples ◽  
Acid Base Status

Changes in uterine fluid composition and acid-base status during shell formation in the chicken

1989 ◽  
Vol 257 (4) ◽  
pp. R732-R737 ◽  
Author(s):  
Z. Arad ◽  
U. Eylath ◽  
M. Ginsburg ◽  
H. Eyal-Giladi
Keyword(s):  
Sharp Increase ◽  
Blood Gases ◽  
Future Application ◽  
Epithelial Tissue ◽  
Fluid Composition ◽  
Acid Base ◽  
Co2 Partial Pressure ◽  
Uterine Fluid ◽  
Acid Base Status

The aim of this study was to characterize the dynamic changes in uterine fluid composition and acid-base status during shell calcification in the chicken. Uterine eggs at timed intervals were manually aborted and the accompanying fluid collected and analyzed for composition of osmolytes, enzymes, and acid-base parameters. Blood samples were analyzed for comparison. No considerable change in blood gases took place in relation to residence time of the calcifying egg in the uterus. A significant acidosis occurred at latter stages. Only minor changes were revealed in plasma osmotic and biochemical composition throughout egg calcification. In contrast, major changes were revealed in uterine fluid composition and acid-base status during calcification. The most prominent phenomenon was the sharp increase in CO2 partial pressure, from 82.2 Torr at 0 h to 132.8 Torr at 10 h. As bicarbonate concentration remained almost stable, fluid pH dropped from 7.412 to 7.250 within this stage. Uterine fluid sodium and chloride concentrations and osmolality dropped significantly in the course of calcification, whereas potassium concentration significantly increased. A sharp increase in glucose, calcium, and magnesium concentrations was measured in the early stages of calcification. These findings are discussed in relation to existing models for transport mechanisms of the uterine epithelial tissue. The comprehensive picture that emerges from the present study should enable future application in establishing a self-contained culturing system in vitro for studies of embryonic development.

Cellular actions of cAMP on HCO3(-)-secreting cells of rabbit CCD: dependence on in vivo acid-base status

1994 ◽  
Vol 266 (4) ◽  
pp. F528-F535 ◽  
Author(s):  
C. Emmons ◽  
J. B. Stokes
Keyword(s):  
Anion Exchanger ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Disulfonic Acid ◽  
Intercalated Cells ◽  
Cortical Collecting Duct ◽  
Acid Base ◽  
Acid Base Status

HCO3- secretion by cortical collecting duct (CCD) occurs via beta-intercalated cells. In vitro CCD HCO3- secretion is modulated by both the in vivo acid-base status of the animal and by adenosine 3',5'-cyclic monophosphate (cAMP). To investigate the mechanism of cAMP-induced HCO3- secretion, we measured intracellular pH (pHi) of individual beta-intercalated cells of CCDs dissected from alkali-loaded rabbits perfused in vitro. beta-Intercalated cells were identified by demonstrating the presence of an apical anion exchanger (cell alkalinization in response to removal of lumen Cl-). After 180 min of perfusion to permit decrease of endogenous cAMP, acute addition of 0.1 mM 8-bromo-cAMP or 1 microM isoproterenol to the bath caused a transient cellular alkalinization (> 0.20 pH units). In the symmetrical absence of either Na+, HCO3-, or Cl-, cAMP produced no change in pHi. Basolateral dihydrogen 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (0.1 mM) for 15 min before cAMP addition also prevented this alkalinization. In contrast to the response of cells from alkali-loaded rabbits, addition of basolateral cAMP to CCDs dissected from normal rabbits resulted in an acidification of beta-intercalated cells (approximately 0.20 pH units). The present studies demonstrate the importance of the in vivo acid-base status of the animal in the regulation of CCD HCO3- secretion by beta-intercalated cells. The results identify the possible existence of a previously unrecognized Na(+)-dependent Cl-/HCO3- exchanger on the basolateral membrane of beta-intercalated cells in alkali-loaded rabbits.

Haemolymph oxygen transport and acid-base status in Glyptonotus antarcticus Eights

Polar Biology ◽  
1997 ◽  
Vol 18 (1) ◽  
pp. 10-15 ◽  
Author(s):  
N. M. Whiteley ◽  
E. W. Taylor ◽  
A. Clarke ◽  
A. J. El Haj
Keyword(s):  
Oxygen Transport ◽  
Acid Base ◽  
Acid Base Status

scholarly journals Effects of ibrutinib on in vitro platelet aggregation in blood samples from healthy donors and donors with platelet dysfunction

Hematology ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 112-117
Author(s):  
Joi Ninomoto ◽  
Ahmad Mokatrin ◽  
Taisei Kinoshita ◽  
Carol Marimpietri ◽  
Terrance D. Barrett ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Platelet Dysfunction ◽  
Blood Samples ◽  
Healthy Donors
Sign in / Sign up

Export Citation Format

Share Document