The nonenzymatic template-directed ligation of oligonucleotides
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Abstract. The nonenzymatic template-directed ligation of oligonucleotides containing 2', 3'-cyclic phosphate was investigated in the presence of divalent cations. Ligation of the oligonucleotides readily occurred in the presence of Mg2+, Mn2+, Co2+, Zn2+, Pb2+. Efficacy of the metal ion catalysts inversely correlated with pKa values of the metal-bound water molecules. The intermolecular transesterification reaction yielded at least 95metal ion. Relatively high reaction yields (up to 15fragmentation to oligonucleotides with 2',3'-cyclic phosphates, followed by reactions of those oligonucleotides could provide a source of new RNA molecules under prebiotic conditions.
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2007 ◽
Vol 47
(2)
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pp. 668-675
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1990 ◽
Vol 38
(11)
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pp. 3147-3154
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2001 ◽
Vol 204
(6)
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pp. 1053-1061
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1991 ◽
Vol 46
(10)
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pp. 1279-1286
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