scholarly journals Application of DVC-FISH method in tracking Escherichia coli in drinking water distribution networks

2013 ◽  
Vol 6 (1) ◽  
pp. 25-31 ◽  
Author(s):  
L. Mezule ◽  
S. Larsson ◽  
T. Juhna
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Distribution System ◽  
Water Distribution ◽  
Water Distribution System ◽  
Distribution Networks ◽  
Water Distribution Networks ◽  
Water Safety ◽  
E Coli ◽  
Drinking Water Distribution

Abstract. Sporadic detection of live (viable) Escherichia coli in drinking water and biofilm with molecular methods but not with standard plate counts has raised concerns about the reliability of this indicator in the surveillance of drinking water safety. The aim of this study was to determine spatial distribution of different viability forms of E. coli in a drinking water distribution system which complies with European Drinking Water Directive (98/83/EC). For two years coupons (two week old) and pre-concentrated (100 times with ultrafilters) water samples were collected after treatment plants and from four sites in the distribution network at several distances. The samples were analyzed for total, viable (able to divide as DVC-FISH positive) and cultivable E. coli. The results showed that low numbers of E. coli enters the distribution sytem from the treatment plants and tend to accumulate in the biofilm of water distribution system. Almost all of the samples contained metabolically active E. coli in the range of 1 to 50 cells per litre or cm2 which represented approximately 53% of all E. coli detected. The amount of viable E. coli significantly increased into the network irrespective of the season. The study has shown that DVC-FISH method in combination with water pre-concentration and biofilm sampling allows to better understand the behaviour of E. coli in water distribution networks, thus, it provides new evidences for water safety control.

scholarly journals Effect of Phosphorus on Survival of Escherichia coli in Drinking Water Biofilms

2007 ◽  
Vol 73 (11) ◽  
pp. 3755-3758 ◽  
Author(s):  
Talis Juhna ◽  
Dagne Birzniece ◽  
Janis Rubulis
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Distribution System ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution System ◽  
Phosphorus Addition ◽  
Drinking Water Distribution Systems ◽  
E Coli ◽  
Drinking Water Distribution

ABSTRACT The effect of phosphorus addition on survival of Escherichia coli in an experimental drinking water distribution system was investigated. Higher phosphorus concentrations prolonged the survival of culturable E. coli in water and biofilms. Although phosphorus addition did not affect viable but not culturable (VBNC) E. coli in biofilms, these structures could act as a reservoir of VBNC forms of E. coli in drinking water distribution systems.

scholarly journals Detection of Escherichia coli in Biofilms from Pipe Samples and Coupons in Drinking Water Distribution Networks

2007 ◽  
Vol 73 (22) ◽  
pp. 7456-7464 ◽  
Author(s):  
T. Juhna ◽  
D. Birzniece ◽  
S. Larsson ◽  
D. Zulenkovs ◽  
A. Sharipo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Cast Iron ◽  
Water Distribution ◽  
Distribution Networks ◽  
Water Distribution Networks ◽  
Bacterial Number ◽  
E Coli ◽  
Drinking Water Distribution ◽  
Enzymatic Methods

ABSTRACT Fluorescence in situ hybridization (FISH) was used for direct detection of Escherichia coli on pipe surfaces and coupons in drinking water distribution networks. Old cast iron main pipes were removed from water distribution networks in France, England, Portugal, and Latvia, and E. coli was analyzed in the biofilm. In addition, 44 flat coupons made of cast iron, polyvinyl chloride, or stainless steel were placed into and continuously exposed to water on 15 locations of 6 distribution networks in France and Latvia and examined after 1 to 6 months exposure to the drinking water. In order to increase the signal intensity, a peptide nucleic acid (PNA) 15-mer probe was used in the FISH screening for the presence or absence of E. coli on the surface of pipes and coupons, thus reducing occasional problems of autofluorescence and low fluorescence of the labeled bacteria. For comparison, cells were removed from the surfaces and examined with culture-based or enzymatic (detection of β-d-glucuronidase) methods. An additional verification was made by using PCR. Culture method indicated presence of E. coli in one of five pipes, whereas all pipes were positive with the FISH methods. E. coli was detected in 56% of the coupons using PNA FISH, but no E. coli was detected using culture or enzymatic methods. PCR analyses confirmed the presence of E. coli in samples that were negative according to culture-based and enzymatic methods. The viability of E. coli cells in the samples was demonstrated by the cell elongation after resuscitation in low-nutrient medium supplemented with pipemidic acid, suggesting that the cells were present in an active but nonculturable state, unable to grow on agar media. E. coli contributed to ca. 0.001 to 0.1% of the total bacterial number in the samples. The presence and number of E. coli did not correlate with any of physical and/or chemical characteristic of the drinking water (e.g., temperature, chlorine, or biodegradable organic matter concentration). We show here that E. coli is present in the biofilms of drinking water networks in Europe. Some of the cells are metabolically active but are often not detected due to limitations of traditionally used culture-based methods, indicating that biofilm should be considered as a reservoir that must be investigated further in order to evaluate the risk for human health.

scholarly journals Application of DVC-FISH method in trackingEscherichia coliin drinking water distribution networks

2012 ◽  
Vol 5 (1) ◽  
pp. 515-532
Author(s):  
L. Mezule ◽  
S. Larsson ◽  
T. Juhna
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Surface Water ◽  
Water Samples ◽  
Water Distribution ◽  
Large City ◽  
Distribution Networks ◽  
Water Distribution Networks ◽  
E Coli ◽  
Drinking Water Distribution

Abstract. Sporadic detection of live (viable) Escherichia coli in drinking water with molecular methods but not with standard plate counts has raised concerns about the reliability of this indicator in the surveillance of drinking water safety. Previous studies have shown that in low numbers metabolically active E. coli can be found in the biofilm of drinking water. The aim of this study was to analyse the distribution of non-cultivable E. coli in the drinking water distribution system meeting microbiological quality standards and evaluate the importance of the biofilm as its harbour. In total 24 biofilm samplings and 40 at least 100 times pre-concentrated water samples were taken over a period of two years from two water treatment plants (surface water and groundwater) and four locations in water distribution network of a large city. Cultivable, total and able to divide (viable) E. coli concentration was measured in all samples. The results showed that none of the network biofilm samples contained cultivable E. coli and less than two cells per 10 l were detected in the pre-concentrated water samples. However, almost all of the samples contained viable E. coli in the range of 1–50 cells per litre or cm2 which represented approximately 53% of all E. coli detected. The amount of viable E. coli was higher in the biofilm after surface water treatment when compared to the outlet from the groundwater plant and the number tended to increase from both treatment plants further into the network irrespective of the season. In conclusion, E. coli in the water supply is not necessarily directly linked to recent faecal contamination and tends to accumulate in the networks where it is less exposed to disinfectants. Thus it can be accepted that biofilm formation in the drinking water distribution networks increases the risk of accumulation of viable but not cultivable E. coli in the system.

scholarly journals Prevalence of Legionella spp. and Escherichia coli in the drinking water distribution system of Wrocław (Poland)

2020 ◽  
Vol 20 (3) ◽  
pp. 1083-1090
Author(s):  
M. Wolf-Baca ◽  
A. Siedlecka
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Distribution System ◽  
Water Distribution ◽  
Tap Water ◽  
Potential Threat ◽  
E Coli ◽  
Drinking Water Distribution

Abstract Drinking water should be free from bacterial pathogens that threaten human health. The most recognised waterborne opportunistic pathogens, dwelling in tap water, are Legionella pneumophila and Escherichia coli. Drinking water samples were tested for the presence of Legionella spp., L. pneumophila, and E. coli in overall sample microbiomes using a quantitative real-time polymerase chain reaction (qPCR) approach. The results indicate a rather low contribution of Legionella spp. in total bacteria in the tested samples, but L. pneumophila was not detected in any sample. E. coli was detected in only one sample, but at a very low level. The qacEΔ1 gene, conferring resistance to quaternary ammonium compounds, was also not detected in any sample. The results point to generally sufficient quality of drinking water, although the presence of Legionella spp. in tap water samples suggests proliferation of these bacteria in heating units, causing a potential threat to consumer health.

Assessment of Lisbon drinking water distribution network biofilm colonization and associated hazards

2008 ◽  
Vol 8 (4) ◽  
pp. 421-426
Author(s):  
J. Menaia ◽  
M. Benoliel ◽  
A. Lopes ◽  
C. Neto ◽  
E. Ferreira ◽  
...  
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Water Distribution ◽  
Water Distribution System ◽  
Water Distribution Network ◽  
Storage Tanks ◽  
Water Pipe ◽  
Cultural Methods ◽  
Drinking Water Distribution ◽  
And Storage

Concerns arise from the possible occurrence of pathogens in drinking water pipe biofilms and storage tank sediments. In these studies, biofilm samples from pipes and sediments from storage tanks of the Lisbon drinking water distribution system were analyzed. Protein determinations and heterotrophic counts on pipe biofilm samples were used to assess the Lisbon network sessile colonization intensity and distribution. Indicator and pathogenic microorganisms were analyzed in pipe biofilm samples, as well as in storage tanks biofilm and sediments, by using cultural methods and PCR, to assess risks. Results have shown that the Lisbon network sessile colonization is relatively weak in intensity. In addition, no meaningful hazards were apparent for both the network biofilm and the storage tanks biofilm and sediments.

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