CONTROL OF CELL ADHESION AND FUNCTIONS USING SELF-ORGANIZED HONEY COMB-PATTERNED POLYMER FILMS

In this study, a combined experimental and computational study of long-term human bladder epithelial cell line HBLAK adhesion and proliferation on five different polymeric surfaces, namely hyaluronic acid, amylose, collagen, polyhydroxybutyrate, and polylactic acid, was performed with the goal to understand the nature of the attraction between various surface materials and a simplified model of the cell surface (transmembrane protein integrin). These biodegradable polymers are frequently used as scaffolds for tissue engineering. During formation of the new tissue, the scaffold polymers are gradually replaced by the natural extracellular matrix of the proliferating cells. Cell adhesion and proliferation experiments were carried out employing thin polymer films prepared by solvent casting while for the computational approach three-dimensional molecular models of layers of ordered polymeric fibers were used as quasi-planar nano-sized models of polymeric surface patches. Experimental results indicated a good capability of amylose, polyhydroxybutyrate, and hyaluronic acid polymer films to foster cell adhesion. Proliferation experiment, carried out by incubating cells with the investigated polymer films for 72 h, showed that all the investigated polymers are able to sustain a good proliferation of HBLAK cells almost comparable to plain glass. Computational estimate of molecular mechanic interaction energies between three-dimensional models of polymeric films and the collagen-binding α2 I domain of the cell adhesion receptor integrin α2β1 confirmed elevated affinity to amylose and polyhydroxybutyrate that is related to higher polarity of function groups on the film surface as documented by the maps of molecular electrostatic potential. This combined experimental and modeling approach can contribute to rational design and surface modifications of polymeric material suitable for forming the scaffolds of human urethra that can be effectively colonized by stem cells.

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Effect of Pore Size of Self-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2007.514 ◽

2007 ◽

Vol 7 (3) ◽

pp. 763-772 ◽

Cited By ~ 65

Author(s):

Masaru Tanaka ◽

Aiko Takayama ◽

Emiko Ito ◽

Hiroshi Sunami ◽

Sadaaki Yamamoto ◽

...

Keyword(s):

Endothelial Cells ◽

Pore Size ◽

Focal Adhesion ◽

Polymer Films ◽

Self Organized ◽

And Function

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Biomimetic liquid repellent surfaces based on self-organized honeycomb-patterned polymer films (Conference Presentation)

Bioinspiration, Biomimetics, and Bioreplication 2017 ◽

10.1117/12.2257397 ◽

2017 ◽

Author(s):

Hiroshi Yabu ◽

Jun Kamei

Keyword(s):

Polymer Films ◽

Self Organized

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In Vitro and In Vivo Studies of Biodegradability and Biocompatibility of Poly(εCL)-b-Poly(EtOEP)-Based Films

Polymers ◽

10.3390/polym12123039 ◽

2020 ◽

Vol 12 (12) ◽

pp. 3039

Author(s):

Ilya Nifant’ev ◽

Andrey Shlyakhtin ◽

Pavel Komarov ◽

Alexander Tavtorkin ◽

Evgeniya Kananykhina ◽

...

Keyword(s):

Cell Adhesion ◽

Block Copolymers ◽

Polymer Films ◽

Hydrolytic Stability ◽

Polymer Materials ◽

In Vivo Studies ◽

Cytotoxicity Test ◽

The Impact

The control of surface bioadhesive properties of the subcutaneous implants is essential for the development of biosensors and controlled drug release devices. Poly(alkyl ethylene phosphate)-based (co)polymers are structurally versatile, biocompatible and biodegradable, and may be regarded as an alternative to poly(ethylene glycol) (PEG) copolymers in the creation of antiadhesive materials. The present work reports the synthesis of block copolymers of ε-caprolactone (εCL) and 2-ethoxy-1,3,2-dioxaphospholane-2-oxide (ethyl ethylene phosphate, EtOEP) with different content of EtOEP fragments, preparation of polymer films, and the results of the study of the impact of EtOEP/εCL ratio on the hydrophilicity (contact angle of wetting), hydrolytic stability, cytotoxicity, protein and cell adhesion, and cell proliferation using umbilical cord multipotent stem cells. It was found that the increase of EtOEP/εCL ratio results in increase of hydrophilicity of the polymer films with lowering of the protein and cell adhesion. MTT cytotoxicity test showed no significant deviations in toxicity of poly(εCL) and poly(εCL)-b-poly(EtOEP)-based films. The influence of the length of poly(EtOEP)chain in block-copolymers on fibrotic reactions was analyzed using subcutaneous implantation experiments (Wistar line rats), the increase of the width of the fibrous capsule correlated with higher EtOEP/εCL ratio. However, the copolymer-based film with highest content of polyphosphate had been subjected to faster degradation with a formation of developed contact surface of poly(εCL). The rate of the degradation of polyphosphate in vivo was significantly higher than the rate of the degradation of polyphosphate in vitro, which only confirms an objective value of in vivo experiments in the development of polymer materials for biomedical applications.

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