scholarly journals Convolution-based Soma Counting Algorithm for Confocal Microscopy Image Stacks

Author(s):  
Shih-Ting Huang ◽  
Yue Jiang ◽  
Hao-Chiang Shao
2016 ◽  
Vol 45 (23) ◽  
pp. 6520-6545 ◽  
Author(s):  
N. Grimaldi ◽  
F. Andrade ◽  
N. Segovia ◽  
L. Ferrer-Tasies ◽  
S. Sala ◽  
...  

Multifunctional lipid-based nanovesicles (L-NVs) prepared by molecular self-assembly of membrane components together with (bio)-active molecules, by means of compressed CO2-media or other non-conventional methods lead to highly homogeneous, tailor-made nanovesicles that are used for advanced nanomedicine. Confocal microscopy image of siRNA transfection using L-NVs, reprinted with permission from de Jonge,et al.,Gene Therapy, 2006,13, 400–411.


PAMM ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 69-70 ◽  
Author(s):  
Pouyan Asgharzadeh ◽  
Bugra Özdemir ◽  
Stefanie J. Müller ◽  
Ralf Reski ◽  
Oliver Röhrle

Author(s):  
Peter Shaw ◽  
Alison Beven ◽  
David Rawlins ◽  
Martin Highett

Fluorescent in situ hybridization and 3-D confocal microscopy have been used to study the arrangement of genes and transcripts in interphase plant nuclei. Wide-field fluorescence microscopy coupled with computer deconvolution has also been used on the same specimens and gives comparable results to confocal imaging. When image deconvolution is applied to confocal images, these show a significant improvement. A comparison of confocal and wide-field (CCD) images of the same specimen (a plant nucleolus labelled with probe to the rRNA genes) is shown in Figure 1. The deconvoluted wide-field data (Fig 1b) and the raw confocal data (Fig 1c) are remarkably similar; the former is somewhat clearer. The deconvoluted confocal data (Fig 1d) is clearest of all.Double-stranded DNA and single-stranded RNA probes to the 45S and 5S RNA genes have been used to investigate the functional organization of the nucleolus in pea root tissue. In situ hybridization was carried out on vibratome slices approximately 50μm thick (about 3 cells).


2015 ◽  
Vol 135 (3) ◽  
pp. 710-717 ◽  
Author(s):  
Sila Kurugol ◽  
Kivanc Kose ◽  
Brian Park ◽  
Jennifer G. Dy ◽  
Dana H. Brooks ◽  
...  

2007 ◽  
Author(s):  
Il-Han Kim ◽  
William J. Godinez ◽  
Nathalie Harder ◽  
Felipe Mora-Bermúdez ◽  
Jan Ellenberg ◽  
...  

2018 ◽  
Vol 45 (3) ◽  
pp. 187-197 ◽  
Author(s):  
Neda Shahriari ◽  
Jane M. Grant-Kels ◽  
Harold Rabinovitz ◽  
Margaret Oliviero ◽  
Alon Scope

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