Filsoniana lhasanensis sp. nov. from Tibet, China

Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 663-675
Author(s):  
Xue-Mei Wen ◽  
Hurnisa Shahidin ◽  
Abdulla Abbas
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Dark Orange ◽  
A New Species

A new species, Filsoniana lhasanensis, is described from Tibet, China, where it grows on rocks at altitudes 4200–4432 m. The lichen is characterised by a poorly developed or absent dark orange areolate thallus, lecanorine to zeorine orange to deep-orange aggregated apothecia, and richly branched and anastomosed paraphyses. DNA was obtained for three gene loci—nuclear internal transcribed spacer (ITS), nuclear large subunit (LSU), and mitochondrial small subunit (SSU). Phylogenetic analyses support the taxon as a new species of Filsoniana .

A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Light Blue ◽  
Ectal Excipulum ◽  
A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

Morphological and phylogenetic analyses reveal a new species of Fistulina (Fistulinaceae, Agaricales) from Australia

Phytotaxa ◽  
2019 ◽  
Vol 420 (3) ◽  
pp. 233-240
Author(s):  
YI-FEI SUN ◽  
SHUN LIU ◽  
BAO-KAI CUI
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Pore Surface ◽  
Ribosomal Rna Gene ◽  
Hyphal System ◽  
Cotton Blue ◽  
A New Species

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.

Gerhardtia sinensis (Agaricales, Lyophyllaceae), a new species and a newly recorded genus for China

Phytotaxa ◽  
2017 ◽  
Vol 332 (2) ◽  
pp. 172 ◽  
Author(s):  
TING LI ◽  
TAIHUI LI ◽  
CHAOQUN WANG ◽  
WANGQIU DENG ◽  
BIN SONG
Keyword(s):  
New Species ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Pale Yellow ◽  
Molecular Phylogenetic ◽  
A New Species

Gerhardtia sinensis, as a new species discovered from southern China, is described, illustrated and compared with morphologically similar and phylogenetically related species. Morphologically, it is characterized by its white to yellowish or partially pale yellow pileus with faint striae, subdistant to fairly distant lamellae with intervenose veins, and slightly verruculose basidiospores (4.7–)5.2–6.2(–6.6) × (2.5–)3.0–3.4(–3.8) μm in size. Molecular phylogenetic analyses of the new species and related taxa are performed based on the sequences of nuclear ribosomal large subunit (nrLSU) and nuclear ribosomal internal transcribed spacer (ITS).

Morphological and molecular data on a new species of Plagiorhynchus Lühe, 1911 (Acanthocephala: Plagiorhynchidae) from the long-billed curlew (Numenius americanus) from northern Mexico

2019 ◽  
Vol 94 ◽  
Author(s):  
M. García-Varela ◽  
J.-K. Park ◽  
J.S. Hernández-Orts ◽  
C.D. Pinacho-Pinacho
Keyword(s):  
New Species ◽  
Marine Mammals ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Molecular Data ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Northern Mexico ◽  
Numenius Americanus ◽  
A New Species

Abstract A new species of the genus Plagiorhynchus Lühe, 1911 from the intestine of the long-billed curlew (Numenius americanus) from northern Mexico is described. Plagiorhynchus (Plagiorhynchus) aznari n. sp. is morphologically distinguished from other congeneric species from the Americas by having a trunk expanded anteriorly and a cylindrical proboscis, armed with 19 longitudinal rows of hooks, with 14–15 hooks each row. Nearly complete sequences of the small subunit and large subunit of the nuclear ribosomal DNA of the new species were determined and compared with available sequences from GenBank. Phylogenetic analyses inferred from the two molecular markers consistently showed that P. (Plagiorhynchus) aznari n. sp. is closely related to P. (Plagiorhynchus) allisonae, and this clade is sister to a clade formed by P. (Prosthorhynchus) transversus and P. (Prosthorhynchus) cylindraceus from Plagiorhynchidae. The new species represents the second record of the genus in Mexico and the fourth species in the Americas. The phylogenetic relationships among the members of the order Polymorphida in this study provide significant insights into the evolution of ecological associations between parasites and their definitive hosts. Our analyses suggest that the colonization of marine mammals, fish-eating birds and waterfowl in Polymorphidae might have occurred independently, from a common ancestor of Centrorhynchidae and Plagiorhynchidae that colonized terrestrial birds and mammals.

Morphological characteristics and phylogenetic analyses revealed a new Calocybe (Lyophyllaceae, Basidiomycota) species from northeast China

Phytotaxa ◽  
2021 ◽  
Vol 490 (2) ◽  
pp. 203-210
Author(s):  
JIZE XU ◽  
XIAODONG YU ◽  
CHUNLAN ZHANG ◽  
YU LI
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Northeast China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Molecular Phylogenetic ◽  
A New Species

A new species, Calocybe decurrens, is illustrated and described in detail based on morphological characteristics and phylogenetic analyses. Calocybe decurrens is mainly characterized by its decurrent gills and by its stipe that discolors upon maturation. Molecular phylogenetic analyses were based on the internal transcribed spacer (ITS1-5.8S-ITS2) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequences. The results indicated that its affiliation is in genus Calocybe, where it occupies an isolated position. A full description, color images, illustrations and a phylogenetic tree to show the placement of the new species are provided.

A new and intriguing brown-spored Leucocoprinus species

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 44-54
Author(s):  
JUAN M. SUAREZ ◽  
ALEXANDRA M. GOTTLIEB ◽  
BERNARDO E. LECHNER
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Buenos Aires ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nuclear Rdna ◽  
Internal Transcribed Spacer Region ◽  
A New Species

Leucocoprinus brunneosporus sp. nov., collected at the Santa Catalina Reserve (Buenos Aires, Argentina), is proposed as a new species. We performed separate phylogenetic analyses of the nuclear rDNA large subunit (28S) and the complete nuclear rDNA internal transcribed spacer region (ITS) to establish the placement of the new species in the phylogeny of the Agaricaceae. This new species has a macromorphology similar to that of Lc. birnbaumii, but the spore print is light brown. Detailed descriptions and illustrations of the macro- and microscopic characters are provided.

Plectania lutea (Sarcosomataceae), a new species from southwestern karst areas of China

Phytotaxa ◽  
2021 ◽  
Vol 509 (3) ◽  
Author(s):  
GUANG-FU MOU ◽  
TOLGOR BAU
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
Internal Transcribed Spacer ◽  
External Surface ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
New Taxon ◽  
Rdna Sequences ◽  
Karst Areas ◽  
A New Species

Plectania lutea, is described as a new species from subtropical karst areas of China. The main distinguishing characteristics of P. lutea are the yellow hymenium, the external surface with ridges, the gelatinous but not semifluid medullary excipulum, and the large finely warted ascospores. The phylogenetic analyses based on internal transcribed spacer (ITS) and the large subunit (LSU) regions of rDNA sequences using Bayesian (BA) and Maximum Likelihood (ML) methods showed that the new taxon can be distinguished from phenotypically similar and phylogenetically related species.

Amanita rufobrunnescens, a new species of Amanita section Amidella from South China

Phytotaxa ◽  
2016 ◽  
Vol 243 (2) ◽  
pp. 147 ◽  
Author(s):  
Wangqiu Deng ◽  
Taihui Li ◽  
MD. IQBAL HOSEN
Keyword(s):  
New Species ◽  
South China ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Guangdong Province ◽  
Distinct Species ◽  
Nuclear Ribosomal Dna ◽  
Molecular Phylogenetic ◽  
A New Species

Amanita rufobrunnescens is described as a new species in the subgenus Lepidella section Amidella from Guangdong Province, China. It is characterized by whitish basidiomes that bruise reddish brown, brownish sub-membranous to fibrillose volval remnants on the pileus, a striate pileus margin, white lamellae with truncate lamellulae, grayish orange to light brown volva, amyloid basidiospores that are ellipsoid to elongate (9.5–)10–12(–13) × (5–)5.5–6.5(–7) µm, and 5–10 μm wide pileipellis hyphae with yellowish vacuolar pigments. There are no clamp connections. Molecular phylogenetic analyses based on the nuclear ribosomal large subunit of nuclear ribosomal DNA (LSU) and internal transcribed spacer (ITS) sequences reveal that A. rufobrunnescens is a distinct species.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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