scholarly journals Influencia de diferentes medios de cultivo y niveles de pH en el crecimiento in vitro de 6 cepas del género Suillus

2021 ◽  
Vol 27 (3) ◽  
pp. 17-33
Author(s):  
Patricio Chung Guin-Po
Keyword(s):  
Pinus Radiata ◽  
Suillus Luteus

Se analizó el comportamiento in vitro de 6 cepas de las especies Suillus luteus, S, granulatus y S. bellinii, asociados a plantaciones de Pinus radiata. Se evaluó las variables crecimiento radial (CR), velocidad media de crecimiento(VMC) y biomasa (B) creciendo en los medios PDA (Papa Dextrosa Agar), MNM (Medio Melin-Norkrans) y BAF (Biotina Aneurina ácido Fólico) y en 5 niveles de pH (4,8, 5,3; 5,8; 6,3; y 6,8).  A nivel de cepas se analizó las interacciones presentes entre los factores medio de cultivo y pH. Se observó comportamientos variables de cada cepa para las variables CR y VMC frente a los factores evaluados.  Para la variable B, se observan promedios significativos para los medios de cultivo PDA y/o BAF, dependiendo de la cepa estudiada, con valores significativos de pH que variaron para cada cepa. Comparaciones entre valores del CR y de B, muestran alta correlación entre ellos para cepas de S. granulatus, baja para cepas de S. luteus y mediana a nula para cepas de S. bellinii.

scholarly journals DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

2015 ◽  
Vol 39 (1) ◽  
pp. 105-113
Author(s):  
Marta González ◽  
Iván Quiroz ◽  
Ruy Travieso ◽  
Patricio Chung ◽  
Edison García
Keyword(s):  
Pinus Radiata ◽  
Eucalyptus Globulus ◽  
Suillus Luteus ◽  
Scleroderma Citrinum

El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN) como en baja presencia de ellos (EMA), y con pH más bien ácido (4,8 y 5,8). Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

Growth promotion of Suillus luteus by adenosine in vitro

Mycoscience ◽  
2010 ◽  
Vol 51 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Haihan Zhang ◽  
Ming Tang ◽  
Hui Chen
Keyword(s):  
Growth Promotion ◽  
Suillus Luteus

scholarly journals Copper-Adapted Suillus luteus, a Symbiotic Solution for Pines Colonizing Cu Mine Spoils

2005 ◽  
Vol 71 (11) ◽  
pp. 7279-7284 ◽  
Author(s):  
K. Adriaensen ◽  
T. Vrålstad ◽  
J.-P. Noben ◽  
J. Vangronsveld ◽  
J. V. Colpaert
Keyword(s):  
Large Scale ◽  
Land Reclamation ◽  
Natural Populations ◽  
Local Environment ◽  
Ectomycorrhizal Fungus ◽  
Suillus Luteus ◽  
Cu Toxicity ◽  
Pine Trees ◽  
Cu Tolerance

ABSTRACT Natural populations thriving in heavy-metal-contaminated ecosystems are often subjected to selective pressures for increased resistance to toxic metals. In the present study we describe a population of the ectomycorrhizal fungus Suillus luteus that colonized a toxic Cu mine spoil in Norway. We hypothesized that this population had developed adaptive Cu tolerance and was able to protect pine trees against Cu toxicity. We also tested for the existence of cotolerance to Cu and Zn in S. luteus. Isolates from Cu-polluted, Zn-polluted, and nonpolluted sites were grown in vitro on Cu- or Zn-supplemented medium. The Cu mine isolates exhibited high Cu tolerance, whereas the Zn-tolerant isolates were shown to be Cu sensitive, and vice versa. This indicates the evolution of metal-specific tolerance mechanisms is strongly triggered by the pollution in the local environment. Cotolerance does not occur in the S. luteus isolates studied. In a dose-response experiment, the Cu sensitivity of nonmycorrhizal Pinus sylvestris seedlings was compared to the sensitivity of mycorrhizal seedlings colonized either by a Cu-sensitive or Cu-tolerant S. luteus isolate. In nonmycorrhizal plants and plants colonized by the Cu-sensitive isolate, root growth and nutrient uptake were strongly inhibited under Cu stress conditions. In contrast, plants colonized by the Cu-tolerant isolate were hardly affected. The Cu-adapted S. luteus isolate provided excellent insurance against Cu toxicity in pine seedlings exposed to elevated Cu levels. Such a metal-adapted Suillus-Pinus combination might be suitable for large-scale land reclamation at phytotoxic metalliferous and industrial sites.

Comparative in vitro and early nursery performance of adventitious shoots from cryopreserved cotyledons and axillary shoots from epicotyls of the same zygotic embryo of control-pollinated Pinus radiata

2005 ◽  
Vol 35 (11) ◽  
pp. 2629-2641 ◽  
Author(s):  
Cathy L Hargreaves ◽  
Lynette J Grace ◽  
Susan A van der Maas ◽  
Mike I Menzies ◽  
Satish Kumar ◽  
...  
Keyword(s):  
Pinus Radiata ◽  
Zygotic Embryo ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Radiata Pine ◽  
Field Trials ◽  
Physiological Age ◽  
In Vitro Growth ◽  
Axillary Shoots

This is the first published report comparing production and performance of adventitious shoots from cryopreserved cotyledons, with axillary shoots formed from epicotyls of the same zygotic embryo of radiata pine (Pinus radiata D. Don). Genotypes from 10 control-pollinated families of P. radiata in two treatments were compared for shoot initiation, in vitro growth, rooting, and early nursery performance. Plant growth in nursery beds was assessed by measuring height after 2 and 7 months. After 8 months in nursery beds, the physiological ages of genotypes were assessed before field planting. Genotype capture was higher from the cryopreserved cotyledons than from the epicotyls. This technique has the advantage of preserving juvenile material while field testing is done. Early shoot multiplication in both treatments was good. After four transfer cycles, epicotyl cultures showed improved elongation and higher multiplication rates. After 6 months of in vitro growth, shoots from both treatments were given auxin pulses. Shoots of adventitious origin were slower to root than epicotyl-derived shoots. Overall rooting rates were satisfactory. Plants of adventitious origin were shorter when planted into nursery beds and when transferred to field trials. Assessment of relative physiological age indicated that all plants of adventitious origin showed some increase.

scholarly journals Intraspecific aluminium response in Suillus luteus (L.) s.f. gray., an ectomycorrhizal symbiont of scots pine

2014 ◽  
Vol 64 (1) ◽  
pp. 97-105 ◽  
Author(s):  
Tomasz Leski ◽  
Maria Rudawska ◽  
Barbara Kieliszewska-Rokicka
Keyword(s):  
Metal Tolerance ◽  
Dry Weight ◽  
Ectomycorrhizal Fungus ◽  
Aluminium Tolerance ◽  
Aluminium Content ◽  
Suillus Luteus ◽  
Agar Media ◽  
A Site ◽  
Different Order

Ten isolates of the ectomycorrhizal fungus <i>Suillus luteus</i> have been cultured on an aluminium containing growth medium in order to determine their <i>in vitro</i> aluminium tolerance. Five isolates originated from a site heavily polluted by acid rain with a high availability of aluminium in the soil. Five others were collected from a site free from direct pollution. Aluminium content in the sporocarps of <i>S. luteus</i> differed according to the site of origin and did not reveal symptoms of bioconcentration, although such phenomena were found when mycelium isolated from the sporocarps was submited to 100 mg/L Al in liquid culture. A clear relationship between Al accumulation <i>in vitro</i> and the site of origin of the isolate was not observed, although the highest amount of Al was found in the mycelium derived from the polluted soil. In a second experiment all isolates were grown in agar media containing 10, 100, 500 and 1000 mg/L<sup>-1</sup> Al and the colony diameter during culture and the final colony dry weight determined.<i> S. luteus</i> appeared to be very tolerant to the presence of Al in the medium. Each of the parameters used to measure the metal tolerance of the fungus ranked the isolates in a slightly different order, but those originating from the polluted area showed some superiority over the others. In polluted soils this species seems to have been submitted to a selection for higher aluminium tolerance. The results are discussed with reference to the possibilities of transformating in vitro studies to situation in the forest ecosystem.

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