scholarly journals Promoter Cloning of Human SETDB1 Gene Utilizing Bioinformatic Programs

2014 ◽  
Vol 24 (1) ◽  
pp. 1-7
Author(s):  
Hee-Jung Noh ◽  
Keun-Cheol Kim
Keyword(s):  
Promoter Cloning

A single-copy promoter-cloning vector for use in Escherichia coli

Gene ◽  
1991 ◽  
Vol 108 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Barbara J. Froehlich ◽  
June R. Scott
Keyword(s):  
Escherichia Coli ◽  
Single Copy ◽  
Cloning Vector ◽  
Promoter Cloning

scholarly journals Promoter Cloning in the Radioresistant BacteriumDeinococcus radiodurans

2001 ◽  
Vol 183 (10) ◽  
pp. 3169-3175 ◽  
Author(s):  
Rob Meima ◽  
Heather M. Rothfuss ◽  
Lindy Gewin ◽  
Mary E. Lidstrom
Keyword(s):  
Deinococcus Radiodurans ◽  
Consensus Sequence ◽  
Initial Study ◽  
Northern Hybridization ◽  
E Coli ◽  
Homologous Sequences ◽  
Promoter Cloning ◽  
Radiation Resistant ◽  
Integrative Vectors ◽  
Bacterial Domain

ABSTRACT Deinococcus radiodurans is a highly radiation-resistant bacterium that is classed in a major subbranch of the bacterial domain. Since very little is known about gene expression in this bacterium, an initial study of promoters was undertaken. In order to isolate promoters and study promoter function, a series of integrative vectors for stable chromosomal insertion in D. radiodurans were developed. These vectors are based onEscherichia coli replicons that are unable to replicate autonomously in D. radiodurans and carry homologous sequences for replacement recombination in the D. radiodurans chromosome. The resulting integration vectors were used to study expression of reporter genes fused to a number of putative promoters that were amplified from the D. radiodurans R1 genome. Further analysis of these and other putative promoters was performed by Northern hybridization and primer extension experiments. In contrast to previous reports, the −10 and −35 regions of these promoters resembled the ς70consensus sequence of E. coli.

scholarly journals Mining Tissue-specific Contigs from Peanut (Arachis hypogaea L.) for Promoter Cloning by Deep Transcriptome Sequencing

2014 ◽  
Vol 55 (10) ◽  
pp. 1793-1801 ◽  
Author(s):  
Lili Geng ◽  
Xiaohong Duan ◽  
Chun Liang ◽  
Changlong Shu ◽  
Fuping Song ◽  
...  
Keyword(s):  
Arachis Hypogaea ◽  
Transcriptome Sequencing ◽  
Tissue Specific ◽  
Promoter Cloning ◽  
Arachis Hypogaea L

Expression of Bacterial β-Galactosidase in Animal Cells

1982 ◽  
Vol 2 (12) ◽  
pp. 1628-1632
Author(s):  
Gynheung An ◽  
Katsuhiko Hidaka ◽  
Louis Siminovitch
Keyword(s):  
Recombinant Plasmid ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Animal Cells ◽  
Early Promoter ◽  
Promoter Cloning

A recombinant plasmid containing the gene for bacterial β-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

scholarly journals Construction of a promoter-cloning vector in Zymomonas mobilis.

1986 ◽  
Vol 50 (11) ◽  
pp. 2959-2961 ◽  
Author(s):  
Hideshi YANASE ◽  
Jun KURII ◽  
Kenzo TONOMURA
Keyword(s):  
Zymomonas Mobilis ◽  
Cloning Vector ◽  
Promoter Cloning

scholarly journals Expression of Bacterial β-Galactosidase in Animal Cells

1982 ◽  
Vol 2 (12) ◽  
pp. 1628-1632 ◽  
Author(s):  
Gynheung An ◽  
Katsuhiko Hidaka ◽  
Louis Siminovitch
Keyword(s):  
Recombinant Plasmid ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Animal Cells ◽  
Early Promoter ◽  
Promoter Cloning

A recombinant plasmid containing the gene for bacterial β-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

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