Effects of sucrose, kinetin, benzoaminopurine, and photoperiod on the weight and number of microtubers in aseptic in vitro culture of Nevsky potato (Solanum tuberosum L.) variety

2021 ◽  
Vol 35 (12) ◽  
pp. 44-49
Author(s):  
A. T. Gizatullina ◽  
Z. Stashevski
Keyword(s):  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Solanum Tuberosum L

scholarly journals 114 In Vitro Multiplication of Potato (Solanum tuberosum L.) as Affected by the Microcutting Characteristics

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 461B-461
Author(s):  
Gerson R. de L. Fortes ◽  
Rosilene França ◽  
Adriana C. M. Dantas
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Light Intensity ◽  
In Vitro Culture ◽  
Solanum Tuberosum L ◽  
Temperate Climate ◽  
Multiplication Rate ◽  
In Vitro Multiplication ◽  
Growth Room

This work was carried out in the Tissue Culture Laboratory of Embrapa Temperate Climate aiming to maximize the protocol for in vitro culture of potato cv. Baronesa. The treatments consisted of multiplication of microcuttings with one, two, or three buds with/without leaves and originated from different regions of the shoot: apical, middle, or basal. Each treatment was repeated five times with each replication composed of five explants that were inoculated in 250-ml flasks with 40 ml of the medium containing MS salts and vitamins added to: sucrose (30 g·L-1), myo-inositol (100 mg·L-1), agar (6 g·L-1). The pH was adjusted to 5.6 before autoclaving. After inoculation, the flasks remained in a growth room at 25 ± 2 °C, 16-h photoperiod, and 19 μmol·m-2·s-1 light intensity provided by cool-white fluorescents lamps. Observations were done every 5 days. Final evaluation was performed after 30 days. It was observed that basal microcuttings provided longer shoots and that microcuttings with leaves bore the best ones. This kind of explant also favored a higher number of shoots, axilary buds, and better multiplication rate. The presence of leaves in the microcutting is important when basal explants are used once it can improve the number of axillary buds and the rate of multiplication. The higher the number of buds in the microcutting the lower the rate of multiplication. The in vitro multiplication of potato could be improved by using one-leaf bud basal microcutting.

scholarly journals Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Miguel Contreras Ramírez ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Contreras ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

scholarly journals Researches Concerning Improving Methods of In Vitro Microtubers Production

2014 ◽  
Vol 71 (1) ◽  
pp. 118-124
Author(s):  
Andreea NISTOR ◽  
Nicoleta CHIRU ◽  
Mihaela CIOLOCA ◽  
Monica POPA
Keyword(s):  
Salicylic Acid ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Solanum Tuberosum L ◽  
Culture Conditions ◽  
Average Weight

Five genotypes of potato (Solanum tuberosum L.) were induced to form microtubers under two in vitro culture conditions (continuos darkness and light). Cultures maintained under continuos darkness (with a temperatue of 170C) had a higher yield with a greater number of microtuber (1.394/plantlet) than those maintained under long days (16 h of light/day) combined with temperature of 200C. In the last case the microtubers were higher, with with the highest average weight of 0.602 g. Three phytohormones it was used: coumarine, benzylaminopurine and salicylic acid. Regarding the average number of microtubers obtained when it was used coumarine and salicylic acid, the highest average number of microtubers was recorded (1,135 microtubers/plantlet). The study had been performed also on two fractions of the of sugar quantity (80 and 140 g/l), but they had less influence compared to the varieties.

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