scholarly journals 114 In Vitro Multiplication of Potato (Solanum tuberosum L.) as Affected by the Microcutting Characteristics

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 461B-461
Author(s):  
Gerson R. de L. Fortes ◽  
Rosilene França ◽  
Adriana C. M. Dantas
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Light Intensity ◽  
In Vitro Culture ◽  
Solanum Tuberosum L ◽  
Temperate Climate ◽  
Multiplication Rate ◽  
In Vitro Multiplication ◽  
Growth Room

This work was carried out in the Tissue Culture Laboratory of Embrapa Temperate Climate aiming to maximize the protocol for in vitro culture of potato cv. Baronesa. The treatments consisted of multiplication of microcuttings with one, two, or three buds with/without leaves and originated from different regions of the shoot: apical, middle, or basal. Each treatment was repeated five times with each replication composed of five explants that were inoculated in 250-ml flasks with 40 ml of the medium containing MS salts and vitamins added to: sucrose (30 g·L-1), myo-inositol (100 mg·L-1), agar (6 g·L-1). The pH was adjusted to 5.6 before autoclaving. After inoculation, the flasks remained in a growth room at 25 ± 2 °C, 16-h photoperiod, and 19 μmol·m-2·s-1 light intensity provided by cool-white fluorescents lamps. Observations were done every 5 days. Final evaluation was performed after 30 days. It was observed that basal microcuttings provided longer shoots and that microcuttings with leaves bore the best ones. This kind of explant also favored a higher number of shoots, axilary buds, and better multiplication rate. The presence of leaves in the microcutting is important when basal explants are used once it can improve the number of axillary buds and the rate of multiplication. The higher the number of buds in the microcutting the lower the rate of multiplication. The in vitro multiplication of potato could be improved by using one-leaf bud basal microcutting.

scholarly journals 317 Establishment and Multiplication of in Vitro Pyrus spp. Cultivars

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 446E-447
Author(s):  
Adriana Cibele de Mesquita Dantas ◽  
Adriano Nunes Nesi ◽  
Lilia Bender Machado ◽  
Janny Haerter ◽  
Gerson Renan de Luces Fortes
Keyword(s):  
Tissue Culture ◽  
Shoot Tips ◽  
Axillary Buds ◽  
Temperate Climate ◽  
In Vitro Multiplication ◽  
Growth Room ◽  
Sodium Hypochloride

The culture of meristems, shoot tips, and axillary buds leads to the method of in vitro multiplication that is easily used and safe to obtain uniform copies with no undesirable variations. This work aimed to propagate five in vitro pear cultivars: Housui, Carrick, Nijisseiki, Packham's Triumph, and Red Bartlett. The work was carried out in the Tissue Culture Laboratory at Embrapa Temperate Climate. The plants were sprayed with benomyl (1.0 mg./L) and agrimicine (2.4 mg/L) in the fields, 2 weeks before the shoots were collected. The shoots were then cut with two buds with no leaves and desinfested with alcohol 70% for 10 s and 1% sodium hypochloride for 20 min, 50 explants, 25 buds, and 25 meristems, were then transferred to test tubes containing MS salts and vitamins, myo-inositol (100.0 mg/L), sucrose (30.0 g/L), agar (6.0 g/L), added to in mg/L: BAP (1.0), GA3 (0.1), and NAA (0.01). Three pear cultivars were used for in vitro multiplication (`Nijisseiki', `Red Bartlett', and `Housui') by using the same basal salt with N reduced to strength, added to (in mg/L): BAP (1.6), NAA (0.16). The material was kept in growth room under 16-h photoperiod, 25 ± 2 °C and 19 μMol·m-2·s-1 of flux radiation. The in vitro contaminations were mainly due to bacteria derived from the bud material (71.5%). Higher oxidation for meristem material was observed for `Carrick' and `Packham`s Triumph'. `Red Bartlett' showed the best results for all the variable studied, although all cultivars in general presented low response.

scholarly journals In Vitro Multiplication of Potato (Solanum tuberosum L.) cv. Cristal II—Microcutting Origin

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 471F-472
Author(s):  
Gerson R. de L. Fortes ◽  
Luciana B. Andrade ◽  
Janine T.C. Faria ◽  
Marisa de F. Oliveira ◽  
Nilvane T.G. Müller
Keyword(s):  
Solanum Tuberosum ◽  
Dry Matter ◽  
Culture Media ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Meristem Culture ◽  
Multiplication Rate ◽  
High Dry Matter ◽  
Growth Room

The potato cultivar Cristal recently released by the CPACT/EMBRAPA Breeding Program has high dry matter and low reduce sugars. These are desirable characteristics as industry processing is concerned. Nevertheless, this is a recalcitrant cultivar. The meristem culture is difficult to establish along with a very low multiplication rate. The aim of this work was to improve the multiplication rate for this cultivar. Two-bud microcuttings derived from apical, mid, and basal regions were inoculated in test tubes with 10 ml MS culture media and vitamins as follows; myo-inositol (100 mg·L–1); sucrose (10 g·L–1). No growth regulator was added. All treatments were placed in a growth room in a 16-hour photoperiod; 25 ± 2°C and 2000 lux. One month later, although it was observed that the final growth was more pronounced for basal microcuttings, no difference could be detected for number of shoots and multiplication rate. It was concluded that it makes no difference whatsoever kind of microcutting is used to start the micropropagation process.

scholarly journals Multiplicação de bastão-do-imperador em resposta a concentrações de BAP e número de subcultivos

2016 ◽  
Vol 22 (1) ◽  
pp. 88
Author(s):  
Eder De Oliveira Santos ◽  
Antonio Anderson De Jesus Rodrigues ◽  
Esdras Rocha da Silva ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Experimental Design ◽  
Light Intensity ◽  
Analysis Of Variance ◽  
Culture Medium ◽  
Multiplication Rate ◽  
Commercial Cultivation ◽  
Growth Room ◽  
Tropical Flowers ◽  
Etlingera Elatior

The large ornamental potential of tropical flowers has stimulated the commercial cultivation of various species. Micropropagation is a viable alternate method of propagation, since it enables obtaining a higher number of seedlings with uniformity and pathogens free. The objective was to evaluate the in vitro multiplication rate of Etlingera elatior cv. Porcelana, using explants obtained from in vitro established seedling shoots, obtained from the 2nd subcultive. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 6.66; 8.88; and 11,10 μM), and the cultures maintained in a growth room with temperature 25.0 ± 2.0 °C under a photoperiod of 12 hours of light and light intensity of 30 μmol.m-2 s-1. The multiplication rate was monthly, according to the four subcultives, totaling 120 days. The experimental design was completely randomized, with four replications, analyzed in a factorial 4 x 6. The data were submitted to analysis of variance and regression. There were significant differences in subcultives and made for BAP concentrations used. For the first subcultive, the concentration of 2.22 μM of BAP afforded a rate of 4.06 sprouts per explant, already in the second and fourth subcultives, with the addition of cytokinin concentration was increased amount of sprouts reaching at a rate of 4.05 and 4.96 shoots/explant in the highest concentration of BAP. The results of the treatments evaluated indicate that the presence of BAP favored sprout emission. The concentrations of 2.22, 8.88 and 11.10 μM this cytokinin promoted the highest multiplication rates in the first, second and fourth subcultives, respectively.

scholarly journals In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress

2017 ◽  
Vol 3 (5) ◽  
pp. 188
Author(s):  
Bilter Anton Sirait ◽  
Rosa Charloq
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Drought Stress ◽  
Banding Pattern ◽  
Solanum Tuberosum L ◽  
In Vitro Study ◽  
Dry Weight ◽  
Leaf Chlorophyll ◽  
Potato Plantlets

<p class="Els-Abstract-text"><em>In vitro</em> preliminary studies is candidate tolerant of potato (<em>Solanum tuberosum</em> L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to <em>in vitro</em> drought stress conditions using Polyethylene Glycol PEG.  This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg · L<sup>–</sup><sup>1</sup>, P2 = 6 000 mg · L<sup>–</sup><sup>1</sup>.  The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but  the increase in  the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09  in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.</p>

scholarly journals Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Miguel Contreras Ramírez ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

Hidrogeles derivados de acrilamida y ácido itacónico como soporte en medios de cultivo de plántulas de papa

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Jesús Contreras ◽  
Jessica Juárez ◽  
Juan Jaimez
Keyword(s):  
Aqueous Solution ◽  
Solanum Tuberosum ◽  
In Vitro Culture ◽  
Nutrient Solution ◽  
Itaconic Acid ◽  
Phosphate Buffer ◽  
Solanum Tuberosum L ◽  
Murashige Skoog ◽  
Poly Acrylamide

Hidrogeles de poliacrilamida (PAAm) y de poli(acrilamida-co-ácido itacónico) (P(AAm-co-AI)) sintetizados en agua y en una solución acuosa de buffer de fosfato al 0,2 M, fueron usados como soporte en los medios de cultivo de tejido vegetal sistema autotrófico hidropónico (SAH) y cultivo in-vitro con solución nutritiva Murashige-Skoog (MS) para la producción de plántulas de papa (Solanum Tuberosum, L). En los bioensayos realizados fue evaluado el comportamiento de los hidrogeles mediante el análisis de las características morfológicas de las plántulas: longitud de la parte aérea, longitud de la raíz, número de entrenudos y biomasa. Estos parámetros fueron analizados aplicando el análisis de varianza ANOVA de dos factores con múltiples muestras, en donde los factores evaluados fueron el tipo de hidrogel y el solvente usado para su síntesis. Los resultados obtenidos muestran que según el medio de cultivo estas variables se ven favorecidas por el uso de un determinado tipo de hidrogel, es decir, en el SAH los hidrogeles de PAAm son los que presentaron mejores resultados desde el punto de vista morfológico y estadístico. Para el cultivo in-vitro con solución nutritiva MS, las propiedades morfológicas de las plántulas, se vieron beneficiadas por el hidrogel de P(AAm-co-AI) sintetizado en la solución acuosa de buffer de fosfato. Palabras clave: Hidrogeles, medios de cultivo, Poliacrilamida, Poli (acrilamida-co-ácido itacónico). ABSTRACT Hydrogels from poly (acrylamide) (PAAm) and of poly (acrylamide-co-itaconic acid) (P (AAm-co-AI)) synthesized in water and in an aqueous solution of phosphate buffer 0.2 M, were used as a support in the vegetable tissue culture media hydroponic autotrophic system (HAS) and in-vitro culture with Murashige-Skoog (MS) nutrient solution for the production of potato seedlings (Solanum Tuberosum, L). In the bioassays carried out, the behavior of the hydrogels was evaluated by analyzing the morphological characteristics of the seedlings: length of the aerial part, length of the root, number of internodes and biomass. These parameters were analyzed by applying the ANOVA variance analysis of two factors with multiple samples, in which the factors evaluated were the type of hydrogel and the solvent used for its synthesis. The results obtained show that, according to the culture medium, these variables are favored by the use of a certain type of hydrogel, which, in the SAH the PAAm hydrogels are the ones that presented the best results from the morphological and statistical point of view. For the in-vitro culture with MS nutrient solution, the morphological properties of the seedlings were advantageous due to the P(AAm-co-AI) hydrogel synthesized in the aqueous solution of phosphate buffer.  Key words: Hydrogels, medium culture, Polyacrylamide, Poly(acrylamide-co-itaconic acid).

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