scholarly journals Genetic Variation of Eight Indonesian Swamp-Buffalo Populations Based on Cytochrome b Gene Marker

2020 ◽  
Vol 43 (1) ◽  
pp. 1-10
Author(s):  
M. Rusdin ◽  
D. D. Solihin ◽  
A. Gunawan ◽  
C. Talib ◽  
C. Sumantri
Keyword(s):  
Genetic Variation ◽  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Gene Marker ◽  
Swamp Buffalo

Genetic Variation Analysis between Wild and Cultured Pangasianodon hypopthalmus Using COI and Cytochrome b Among Asian Countries

2022 ◽  
Vol 16 (1) ◽  
pp. 84
Author(s):  
Nik Azwarina R Azmi ◽  
Lyena Watty Zuraine Ahmad ◽  
Roziah Kambol ◽  
Sharifah Aminah Syed Mohamad ◽  
Farizan Aris ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Cytochrome B ◽  
Phylogenetic Trees ◽  
Cytochrome B Gene ◽  
Asian Countries ◽  
Base Pairs ◽  
Partial Region ◽  
Pangasianodon Hypophthalmus ◽  
Wide Range ◽  
Striped Catfish

One of the top species in the aquaculture sector, known as striped catfish or Pangasianodon hypophthalmus, is an important and valuable freshwater fish in many countries. Due to the high demand for this species, their number has declined to "threatened" levels.  Hence, the purpose of this study is to analyse the genetic variation of wild and cultured striped catfish collected from five producers in Asian countries; Thailand, Vietnam, Indonesia, India, and Philippines, by using mitochondrial DNA partial region data sequence; CO1 and cytochrome b gene. Population analyses using 395 base pairs length for CO1 and 275 base pairs length of cytochrome b partial region nucleotide sequence have shown no significance difference between wild and cultured striped catfish. Vietnam species had shown a wide range of genetic distance of the intrapopulation compared with other countries in the range of 0.000-0.040 for CO1 gene and 0.003-0.008 for cytochrome b gene. The Neighbour-joining method has also been used to construct phylogenetic trees using CO1 gene; the tree formed few subclades with mixed populations, and the tree using cytochrome b showed only Vietnam species divided into a few sub-populations. For the other four countries, Thailand, Indonesia, India, and Philippines were in the same group. Hence, this study's findings may provide a reference for inter and intra-relationships of P. hypophthalmus that may help in the aquaculture activity of this striped catfish.

Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

2017 ◽  
Author(s):  
Junaid Naseer ◽  
Khalid Mahmood Anjum ◽  
Waseem Ahmad Khan ◽  
Muhammad Imran ◽  
Muhammad Ishaque ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Private Sector ◽  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Universal Primers ◽  
Sample Collection ◽  
Government Sector ◽  
Pavo Cristatus ◽  
Cyt B Gene ◽  
Breeding Farms

This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from Government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity ³95% rather than bird of private sector with ³90% homology with reference Accession No. More genetic variation, which is the guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.

Genetic diversity of mitochondrial cytochrome b gene in swamp buffalo

2020 ◽  
Vol 51 (6) ◽  
pp. 977-981
Author(s):  
T. Sun ◽  
S. Wang ◽  
Q. Hanif ◽  
N. Chen ◽  
H. Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
Swamp Buffalo

scholarly journals Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Agus Nuryanto ◽  
Nael Huda Qonita ◽  
Hendro Pramono ◽  
Kusbiyanto Kusbiyanto ◽  
Petrus Hary Tjahja Soedibja
Keyword(s):  
Genetic Variation ◽  
Body Size ◽  
Cytochrome B ◽  
Restriction Enzymes ◽  
Cytochrome B Gene ◽  
Rflp Marker ◽  
Rflp Markers ◽  
Molecular Technique ◽  
Pcr Rflp ◽  
Body Sizes

<p>Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. Ther result showed that PCR-RFLP markers of Cytochrome b-<em>Hinf</em>I of 315bp, and 210bp, and also Cytochrome b-<em>Alu</em>I of 334bp and 189bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-<em> Hinf</em>I 366bp, and 159bp and Cytochrome b-<em>Alu</em>I 525bp fragments. It is observed that genetic variation of Cytochrome b-<em>Hinf</em>I and -<em>Alu</em>I markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-<em>Hinf</em>I and -<em>Alu</em>I gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. </p>

Genetic variation of the cytochrome b gene in the rosy bitterling, Rhodeus ocellatus (Cyprinidae) in Japan

2001 ◽  
Vol 48 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Kenji Miyake ◽  
Hidenori Tachida ◽  
Yuji Oshima ◽  
Ryoichi Arai ◽  
Seir&#x000F4; Kimura ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Rhodeus Ocellatus
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