scholarly journals Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Agus Nuryanto ◽  
Nael Huda Qonita ◽  
Hendro Pramono ◽  
Kusbiyanto Kusbiyanto ◽  
Petrus Hary Tjahja Soedibja
Keyword(s):  
Genetic Variation ◽  
Body Size ◽  
Cytochrome B ◽  
Restriction Enzymes ◽  
Cytochrome B Gene ◽  
Rflp Marker ◽  
Rflp Markers ◽  
Molecular Technique ◽  
Pcr Rflp ◽  
Body Sizes

<p>Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. Ther result showed that PCR-RFLP markers of Cytochrome b-<em>Hinf</em>I of 315bp, and 210bp, and also Cytochrome b-<em>Alu</em>I of 334bp and 189bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-<em> Hinf</em>I 366bp, and 159bp and Cytochrome b-<em>Alu</em>I 525bp fragments. It is observed that genetic variation of Cytochrome b-<em>Hinf</em>I and -<em>Alu</em>I markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-<em>Hinf</em>I and -<em>Alu</em>I gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. </p>

scholarly journals Molecular taxonomy of Plagioscion Heckel (Perciformes, Sciaenidae) and evidence from mtDNA RFLP markers for an invasive species in the Paraná river, Southern Brazil

2006 ◽  
Vol 23 (4) ◽  
pp. 1235-1242 ◽  
Author(s):  
Rodrigo A. Torres
Keyword(s):  
Cytochrome B ◽  
Molecular Taxonomy ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Paraná River ◽  
Cytochrome B Gene ◽  
Rflp Markers ◽  
Natural Environments ◽  
Southern Brazil ◽  
Parana River

Mitochondrial RFLP markers were developed to examine whether Plagioscion squamosissimus (Heckel, 1840) is invasive in natural environments of the congener P. ternetzi in the Paraná river, in southern Brazil. Specimens of P. squamosissimus and of the putative P. ternetzi (Boulenger, 1895) were obtained from the Negro river (Manaus, Amazonas, Brazil) and from Paraná river, respectively. Fragments of the cytochrome b gene (900bp) were amplified by PCR and four restriction enzymes (Eco RI, Mbo I, Bam HI and Alu I) yielded the mitochondrial markers. An additional RFLP analysis with a cytochrome b gene sequence of Plagioncion sp. from GeneBank was carried out to validate the prior analysis. No genetic differentiation was found among either sample. While molecular variation in the cytochrome b analysis was no substantial among individuals, the combined analysis was important for demonstrating that there is no evidence for differentiation of the putative sample P. ternetzi from that of P. squamosissimus. The ecological implications of the introduced occurrence of P. squamosissimus, as well as the role of molecular taxonomic approaches for biodiversity studies are discussed.

scholarly journals Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

2002 ◽  
Vol 27 (6) ◽  
pp. 639-643 ◽  
Author(s):  
RITA C. B. WEIKERT-OLIVEIRA ◽  
M. APARECIDA DE RESENDE ◽  
HENRIQUE M. VALÉRIO ◽  
RACHEL B. CALIGIORNE ◽  
EDILSON PAIVA
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variation ◽  
Climatic Factors ◽  
Rapd Analysis ◽  
Its Region ◽  
Restriction Enzymes ◽  
Fungal Species ◽  
High Similarity ◽  
Pcr Rflp ◽  
Upgma Phenogram

Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism among isolates of the same species. A UPGMA phenogram grouped the isolates according to the species and their host plant. RAPD profiles did not reveal polymorphism that directly correlated climatic factors with geographic source of the isolates of B. sorokiniana, and B. oryzae. Teleomorphic species revealed high similarity with their correspondent anamorphs.

A simple method for domestic animal identification in Argentina using PCR-RFLP analysis of cytochrome b gene

2004 ◽  
Vol 6 (4) ◽  
pp. 246-251 ◽  
Author(s):  
Claudio M. Bravi ◽  
Juan P. Lirón ◽  
Patricia M. Mirol ◽  
María V. Ripoli ◽  
Pilar Peral-García ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Rflp Analysis ◽  
Domestic Animal ◽  
Cytochrome B Gene ◽  
Simple Method ◽  
Pcr Rflp ◽  
Animal Identification

Molecular identification of cephalopod species by FINS and PCR-RFLP of a cytochrome b gene fragment

2003 ◽  
Vol 217 (6) ◽  
pp. 524-529 ◽  
Author(s):  
Mar�a Jos� Chapela ◽  
Carmen G. Sotelo ◽  
Ricardo I. P�rez-Mart�n
Keyword(s):  
Cytochrome B ◽  
Molecular Identification ◽  
Cytochrome B Gene ◽  
Gene Fragment ◽  
Cephalopod Species ◽  
Pcr Rflp

Genetic Variation Analysis between Wild and Cultured Pangasianodon hypopthalmus Using COI and Cytochrome b Among Asian Countries

2022 ◽  
Vol 16 (1) ◽  
pp. 84
Author(s):  
Nik Azwarina R Azmi ◽  
Lyena Watty Zuraine Ahmad ◽  
Roziah Kambol ◽  
Sharifah Aminah Syed Mohamad ◽  
Farizan Aris ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Cytochrome B ◽  
Phylogenetic Trees ◽  
Cytochrome B Gene ◽  
Asian Countries ◽  
Base Pairs ◽  
Partial Region ◽  
Pangasianodon Hypophthalmus ◽  
Wide Range ◽  
Striped Catfish

One of the top species in the aquaculture sector, known as striped catfish or Pangasianodon hypophthalmus, is an important and valuable freshwater fish in many countries. Due to the high demand for this species, their number has declined to "threatened" levels.  Hence, the purpose of this study is to analyse the genetic variation of wild and cultured striped catfish collected from five producers in Asian countries; Thailand, Vietnam, Indonesia, India, and Philippines, by using mitochondrial DNA partial region data sequence; CO1 and cytochrome b gene. Population analyses using 395 base pairs length for CO1 and 275 base pairs length of cytochrome b partial region nucleotide sequence have shown no significance difference between wild and cultured striped catfish. Vietnam species had shown a wide range of genetic distance of the intrapopulation compared with other countries in the range of 0.000-0.040 for CO1 gene and 0.003-0.008 for cytochrome b gene. The Neighbour-joining method has also been used to construct phylogenetic trees using CO1 gene; the tree formed few subclades with mixed populations, and the tree using cytochrome b showed only Vietnam species divided into a few sub-populations. For the other four countries, Thailand, Indonesia, India, and Philippines were in the same group. Hence, this study's findings may provide a reference for inter and intra-relationships of P. hypophthalmus that may help in the aquaculture activity of this striped catfish.

Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

2017 ◽  
Author(s):  
Junaid Naseer ◽  
Khalid Mahmood Anjum ◽  
Waseem Ahmad Khan ◽  
Muhammad Imran ◽  
Muhammad Ishaque ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Private Sector ◽  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Universal Primers ◽  
Sample Collection ◽  
Government Sector ◽  
Pavo Cristatus ◽  
Cyt B Gene ◽  
Breeding Farms

This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from Government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity ³95% rather than bird of private sector with ³90% homology with reference Accession No. More genetic variation, which is the guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.

PCR-RFLP analysis of the cytochrome b gene in horse mitochondrial DNA

2009 ◽  
Vol 27 (5) ◽  
pp. 359-363 ◽  
Author(s):  
N Ishida ◽  
T Hasegawa ◽  
H Mukoyama ◽  
T Oyunsuren
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Rflp Analysis ◽  
Cytochrome B Gene ◽  
Pcr Rflp

scholarly journals Application of PCR – RFLP markers for identification of genetically delimited groups of the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae)

2015 ◽  
Vol 38 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Katarzyna Buczkowska
Keyword(s):  
North America ◽  
Scar Marker ◽  
Complex Structure ◽  
Restriction Enzymes ◽  
European Part ◽  
Rflp Markers ◽  
Genetic Studies ◽  
Pcr Product ◽  
Pcr Rflp ◽  
Restriction Sites

AbstractCurrently, two subspecies are formally recognized within Calypogeia fissa: C. fissa subsp. fissa occurring in Europe and C. fissa subsp. neogea known from North America. Genetic studies have revealed a complex structure of this species. Within the European part of distribution, three genetically distinct groups PS, PBand G are distinguished. The combination of the SCAR marker Cal04 and PCR-RFLP markers with three restriction enzymes (SmaI, TaqI and TspGWI) allowed the recognition of all groups within the C. fissa complex. The TaqI enzyme recognizing the restriction sites in the PCR product of SCAR marker Ca104 turned out to be the best marker

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