Genetic Variation Analysis between Wild and Cultured Pangasianodon hypopthalmus Using COI and Cytochrome b  Among Asian Countries

One of the top species in the aquaculture sector, known as striped catfish or Pangasianodon hypophthalmus, is an important and valuable freshwater fish in many countries. Due to the high demand for this species, their number has declined to "threatened" levels. Hence, the purpose of this study is to analyse the genetic variation of wild and cultured striped catfish collected from five producers in Asian countries; Thailand, Vietnam, Indonesia, India, and Philippines, by using mitochondrial DNA partial region data sequence; CO1 and cytochrome b gene. Population analyses using 395 base pairs length for CO1 and 275 base pairs length of cytochrome b partial region nucleotide sequence have shown no significance difference between wild and cultured striped catfish. Vietnam species had shown a wide range of genetic distance of the intrapopulation compared with other countries in the range of 0.000-0.040 for CO1 gene and 0.003-0.008 for cytochrome b gene. The Neighbour-joining method has also been used to construct phylogenetic trees using CO1 gene; the tree formed few subclades with mixed populations, and the tree using cytochrome b showed only Vietnam species divided into a few sub-populations. For the other four countries, Thailand, Indonesia, India, and Philippines were in the same group. Hence, this study's findings may provide a reference for inter and intra-relationships of P. hypophthalmus that may help in the aquaculture activity of this striped catfish.

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Genetic Variation Study within (Ompok bimaculatus and Clupisoma sinensis) Populations in Samarra Dam Middle of Iraq and Shatt Al-Arab Southern of Iraq belonging to Tigris River Using Mitochondrial Cytochrome b Gene

Genetics of Aquatic Organisms ◽

10.4194/2459-1831-v3_2_03 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Rabeeha Mankhi Jebur

Keyword(s):

Genetic Variation ◽

Cytochrome B ◽

Cytochrome B Gene ◽

Mitochondrial Cytochrome ◽

Tigris River ◽

Mitochondrial Cytochrome B ◽

Mitochondrial Cytochrome B Gene ◽

Ompok Bimaculatus

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Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.9150 ◽

2017 ◽

Author(s):

Junaid Naseer ◽

Khalid Mahmood Anjum ◽

Waseem Ahmad Khan ◽

Muhammad Imran ◽

Muhammad Ishaque ◽

...

Keyword(s):

Genetic Variation ◽

Private Sector ◽

Cytochrome B ◽

Cytochrome B Gene ◽

Universal Primers ◽

Sample Collection ◽

Government Sector ◽

Pavo Cristatus ◽

Cyt B Gene ◽

Breeding Farms

This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from Government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity ³95% rather than bird of private sector with ³90% homology with reference Accession No. More genetic variation, which is the guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.

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Phylogenetic relationships among megachiropteran species from the two major islands of the Philippines, deduced from DNA sequences of the cytochrome b gene

Canadian Journal of Zoology ◽

10.1139/z01-125 ◽

2001 ◽

Vol 79 (9) ◽

pp. 1671-1677 ◽

Cited By ~ 3

Author(s):

Severo T Bastian, Jr. ◽

Kazuaki Tanaka ◽

Rea Victoria P Anunciado ◽

Nelson G Natural ◽

Augusto C Sumalde ◽

...

Keyword(s):

Cytochrome B ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

The Philippines ◽

Cytochrome B Gene ◽

Likelihood Methods ◽

Base Pairs ◽

Maximum Likelihood Methods ◽

Pteropus Vampyrus ◽

Cynopterus Brachyotis

Complete cytochrome b gene sequences (1140 base pairs) in species of Megachiroptera were ascertained in order to deduce their phylogenetic relationships, using samples of Cynopterus brachyotis, Eonycteris spelaea, Ptenochirus jagori, Pteropus vampyrus, and Rousettus amplexicaudatus collected from the islands of Luzon and Mindanao in the Philippines. Genetic divergence between samples of R. amplexicaudatus, E. spelaea, and C. brachyotis was very small. On the other hand, a large genetic distance was detected between species of Megachiroptera. The phylogenetic tree using neighbor-joining, parsimony, and maximum-likelihood methods generated similar topologies, reflecting the evolutionary associations among megachiropteran species. We estimated that Megachiroptera separated from Microchiroptera 50.2 million years ago (MYA), and split further approximately 32.4 MYA, forming three lineages: E. spelaea, R. amplexicaudatus, and P. vampyrus and the P. jagori C. brachyotis cluster. The third lineage, composed of P. vampyrus and the P. jagori C. brachyotis cluster, branched out 31.9 MYA. We hypothesize that R. amplexi caudatus diverged from the three members of the subfamily Pteropodinae examined, and its phylogenetic relationship with E. spelaea remains unclear.

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Tawny Owl (Strix aluco) and Hume's Tawny Owl (Strix butleri) Are Distinct Species: Evidence from Nucleotide Sequences of the Cytochrome b Gene

Zeitschrift für Naturforschung C ◽

10.1515/znc-1994-3-411 ◽

1994 ◽

Vol 49 (3-4) ◽

pp. 230-234 ◽

Cited By ~ 12

Author(s):

Petra Heidrich ◽

Michael Wink

Keyword(s):

Cytochrome B ◽

Distinct Species ◽

Cytochrome B Gene ◽

Tyto Alba ◽

Nucleotide Substitutions ◽

Base Pairs ◽

Tawny Owl ◽

Strix Aluco ◽

Parsimony Principle ◽

Athene Noctua

The cytochrome b gene of the Tawny Owl (Strix aluco), Hume’s Tawny Owl (Strix butleri) and the African wood owl (Strix woodfordii) was amplified by polymerase chain reaction (PCR) and partially sequenced (300 base pairs). Sequences differ substantially (9 to 12% nucleotide substitutions) between these taxa indicating that they represent distinct species, which is also implicated from morphological and biogeographic differences. Using cytochrome b sequences of S. aluco, S. butleri, S. woodfordii, Athene noctua and Tyto alba phylogenetic relationship were reconstructed using the “maximum parsimony” principle (PAUP 3.1.1) and the neighbour-joining method (MEGA)

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GENETIC DIVERSITY ANALYSIS OF the first and second generations OF FAST-GROWING STRIPED CATFISH (Pangasianodon hypophthalmus Sauvage,1878) USING MICROSATELLITE ANALYSIS

Indonesian Aquaculture Journal ◽

10.15578/iaj.13.1.2018.1-6 ◽

2018 ◽

Vol 13 (1) ◽

pp. 1

Author(s):

Huria Marnis ◽

Evi Tahapari ◽

Jadmiko Darmawan

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Selective Breeding ◽

Microsatellite Analysis ◽

Breeding Program ◽

Diversity Analysis ◽

Pangasianodon Hypophthalmus ◽

Fast Growing ◽

Striped Catfish ◽

Inbreeding Level

Genetic diversity is an important aspect of a selective breeding program to produce fish broodstock carrying superior traits such as fast-growing, disease resistant, and other traits. We have carried out a breeding program to produce a fast-growing striped catfish (Pangasianodon hypophthalmus) since 2010. The aim of this study was to evaluate the genetic variation of the first (G-1) and second (G-2) generations of fast-growing striped catfish using microsatellite analysis. The G-1 and G-2 populations were selected individually from populations. DNA samples were collected from 40 ind. fish of each population and analyzed using five microsatellite loci (Pg1, Pg2, Pg3, Pg13, and Pg14). The results showed that the number of alleles per loci in the G-1 and G2 populations ranged from 4 to 7 alleles, with an average of five for each generation. The average of observed heterozygosity of the G-1 population (0.420) was lower than the G-2 population (0.495). Inbreeding level showed that the G-1 population was more inbred than the G-2 population. The study also found that both striped catfish populations had relatively low genetic variation. This result suggests that monitoring of genetic variation and better scheme of good spawning were needed on the next selection program to produce the intended fast-growing striped catfish.

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Genetic Variation of Eight Indonesian Swamp-Buffalo Populations Based on Cytochrome b Gene Marker

Tropical Animal Science Journal ◽

10.5398/tasj.2020.43.1.1 ◽

2020 ◽

Vol 43 (1) ◽

pp. 1-10

Author(s):

M. Rusdin ◽

D. D. Solihin ◽

A. Gunawan ◽

C. Talib ◽

C. Sumantri

Keyword(s):

Genetic Variation ◽

Cytochrome B ◽

Cytochrome B Gene ◽

Gene Marker ◽

Swamp Buffalo

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Study of Morphology, Prevalence and Mean Intensity of Monogeneans Infecting Pond Reared Pangasianodon hypophthalmus (Striped Catfish) in Besut, Terengganu

Universiti Malaysia Terengganu Journal of Undergraduate Research ◽

10.46754/umtjur.v1i1.49 ◽

2019 ◽

Vol 1 (1) ◽

pp. 33-40

Author(s):

Sarah Salehah ◽

Faizah Sharom

Keyword(s):

Southeast Asia ◽

Morphological Study ◽

Asian Countries ◽

Monogenean Parasite ◽

Geographic Locations ◽

Mean Intensity ◽

Pangasianodon Hypophthalmus ◽

Gill Filaments ◽

Striped Catfish

This study was conducted to determine the morphology, prevalence and mean intensity of monogeneans on gill filaments of striped catfish Pangasianodon hypophthalmus (Pangasiidae). P. hypophthalmus are important economic fish in Southeast Asia region and predominantly produced in Asian countries such as Malaysia, Bangladesh, Indonesia, Vietnam, Laos, China and Cambodia through aquaculture. Thirty fish were sampled from an earthen pond located in Kuala Besut, Terengganu, in the period from January to February, 2018. The monogenean parasites were removed from the gill filaments and counted. For the morphological study, the extracted monogeneans were mounted on a slide by using drop of ammonium picrate-glycerin (APG) and then were observed under the Compound Advanced Research Microscope. The drawing was done with the aid of lucida camera attached to compound microscope. The monogenean parasite was identified as Thaparocleidus sp based on the characteristics of the morphology and morphometrics of the parasite, which did not differ significantly from the previous descriptions of the same species discovered in other geographic locations. The prevalence and mean intensity levels were 100% and 106.07 parasites per fish, respectively. The monogenean parasites discovered throughout this examination were recorded and the data obtained was summarized.

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Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy Lacepede, 1801) from Single Spawning

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i2.9301 ◽

2017 ◽

Vol 9 (2) ◽

Author(s):

Agus Nuryanto ◽

Nael Huda Qonita ◽

Hendro Pramono ◽

Kusbiyanto Kusbiyanto ◽

Petrus Hary Tjahja Soedibja

Keyword(s):

Genetic Variation ◽

Body Size ◽

Cytochrome B ◽

Restriction Enzymes ◽

Cytochrome B Gene ◽

Rflp Marker ◽

Rflp Markers ◽

Molecular Technique ◽

Pcr Rflp ◽

Body Sizes

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. Ther result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315bp, and 210bp, and also Cytochrome b-AluI of 334bp and 189bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366bp, and 159bp and Cytochrome b-AluI 525bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.

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