scholarly journals Incidence of Listeria spp. in Dairy Cows Feed and Raw Milk in Latvia

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
I. H. Konosonoka ◽  
A. Jemeljanovs ◽  
B. Osmane ◽  
D. Ikauniece ◽  
G. Gulbe
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Farm ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Fresh Milk ◽  
Bulk Milk ◽  
Listeria Ivanovii ◽  
Organic Dairy ◽  
Listeria Seeligeri ◽  
Feed Samples

Feed is a risk factor for poisoning the farm environment thus also fresh milk with pathogenic microorganisms of Listeria genus species. Listeria ivanovii, Listeria innocua, and Listeria seeligeri were isolated from 9.2%, but Listeria monocytogenes from 20.0% of feed samples. Most often different fodders (9.3%) and silage (4.7%) were contaminated with Listeria monocytogenes. Listeria genus species were isolated more often from feed prepared and used in organic dairy farm than from that used in conventional dairy farm, correspondingly 44.4% and 18.3%. No Listeria monocytogenes was found in bulk milk samples of organic dairy farm.

Isolation de micro-organismes du genre Listeria à partir de lait cru destiné à la consommation humaine

1985 ◽  
Vol 31 (10) ◽  
pp. 938-941 ◽  
Author(s):  
Lucas Domínguez Rodriguez ◽  
Jose F. Fernández Garayzabal ◽  
Jose A. Vazquez Boland ◽  
Elias Rodriguez Ferri ◽  
Guillermo Suarez Fernández
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Central Spain ◽  
Listeria Ivanovii ◽  
Lait Cru ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Refrigerated mixtures of raw milk provided by a dairy which was supplied by farms from west and central Spain were tested for the presence of Listeria microorganisms. A total of 95 samples were taken at regular intervals over a 16-month period. Listeria grayi was isolated from 89.5% of the samples, Listeria monocytogenes s. str. from 45.3%, Listeria innocua from 15.8%, Listeria welshimeri from 3.1%, and Listeria seeligeri from 1.05%. Listeria ivanovii, Listeria murrayi, and Listeria denitrificans were not isolated.[Translated by the journal]

Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods

1987 ◽  
Vol 50 (6) ◽  
pp. 479-484 ◽  
Author(s):  
JEFFREY M. FARBER ◽  
JOAN I. SPEIRS
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Enzyme Immunoassay ◽  
Listeria Innocua ◽  
Milk Samples ◽  
Listeria Ivanovii ◽  
Flagellar Antigen ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Monoclonal antibodies directed against antigens of Listeria spp. were produced. Three main classes of immunoglobulins were found that reacted with Listeria strains containing either the A, B, or C flagellar antigen. These antibodies reacted with Listeria monocytogenes, Listeria welshimeri, Listeria seeligeri, Listeria ivanovii and Listeria innocua, but not Listeria grayi, Listeria murrayi or Listeria denitrificans. The monoclones tested did not cross-react with any of the 30 non-Listeria cultures examined, including Staphylococcus aureus and Streptococcus faecalis. Cheese and milk samples naturally-contaminated with L. monocytogenes were found to be positive for Listeria within two working days after initiation by using the monoclonal antibodies in an enzyme immunoassay.

Listeria Species in Raw and Ready-to-Eat Foods from Restaurants

2001 ◽  
Vol 64 (4) ◽  
pp. 551-553 ◽  
Author(s):  
J. M. SORIANO ◽  
H. RICO ◽  
J. C. MOLTÓ ◽  
J. MAÑES
Keyword(s):  
Listeria Monocytogenes ◽  
Listeria Innocua ◽  
Listeria Species ◽  
Listeria Ivanovii ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

From September 1999 to March 2000, meat (pork, beef, and chicken), fish (salmon, hake, and sole), vegetable (lettuce and spinach), and Spanish potato omelette samples obtained at restaurants were collected and tested for the occurrence of Listeria spp. Listeria monocytogenes was isolated from 3 (2.9%) out of 103 studied samples. Other species isolated were Listeria grayi (13.6%), Listeria innocua (1.9%), Listeria ivanovii (5.8%), Listeria seeligeri (3.9%), and Listeria welshimeri (1.9%). Listeria was neither isolated from beef nor any type of fish.

Incidence of Listeria monocytogenes and other Listeria spp. in Ewes' Raw Milk

1994 ◽  
Vol 57 (7) ◽  
pp. 571-575 ◽  
Author(s):  
JOSE L. RODRIGUEZ ◽  
PILAR GAVA ◽  
MARGARITA MEDINA ◽  
MANUEL NUÑEZ
Keyword(s):  
Listeria Monocytogenes ◽  
Sampling Period ◽  
Raw Milk ◽  
Seasonal Influence ◽  
Listeria Ivanovii ◽  
Milk Contamination ◽  
One Year ◽  
The One ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Ewes' milk samples from 287 farm bulk tanks and 17 transport tankers were analyzed for Listeria over a one-year period. Listeria monocytogenes and Listeria innocua were detected in 2.19% and 2.00% of 1052 farm samples, and in 18.38% and 11.76% of 136 tanker samples, respectively. Incidence of Listeria grayi, Listeria ivanovii, Listeria seeligeri and Listeria welshimeri was under 0.4% in farm samples and under 1% in tanker samples. Most farms (93.38%) produced milk free from L. monocytogenes throughout the one-year sampling period. No seasonal influence on milk contamination by Listeria was found. However, ewes' milk contamination by L. monocytogenes and other Listeria spp. was significantly higher in farms where cows were also reared than in farms where only ewes were present.

Prevalence and Contamination Levels of Listeria monocytogenes in Smoked Fish and Pâté Sold in Spain

2001 ◽  
Vol 64 (12) ◽  
pp. 2075-2077 ◽  
Author(s):  
C. DOMINGUEZ ◽  
I. GOMEZ ◽  
J. ZUMALACARREGUI
Keyword(s):  
Listeria Monocytogenes ◽  
Listeria Innocua ◽  
Smoked Fish ◽  
Listeria Ivanovii ◽  
Retail Outlets ◽  
Contamination Levels ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

From March to November 2000, 170 samples of smoked fish and 182 samples of pâté for sale in retail outlets and supermarkets in the nine provinces of Castilla and León (Spain) were analyzed for the prevalence of Listeria monocytogenes and other Listeria spp. L. monocytogenes was isolated from 38 (22.3%) of the 170 samples of smoked fish analyzed. Twenty of these positive samples contained L. monocytogenes at >100 CFU/g. Other Listeria spp., such as Listeria innocua (26 isolates), Listeria grayi (9), Listeria welshimeri (3), Listeria seeligeri (3), and Listeria ivanovii (2), were also detected. L. monocytogenes was isolated from 5.4% of the 182 samples of pâté. Only 1 of the 10 positive samples harbored >100 L. monocytogenes CFU/g. Two other species of Listeria were observed in pâté: L. innocua (12 isolates) and L. grayi (2).

scholarly journals Surveillance for Contamination of Salad with Listeria Monocytogenes in Some of Baghdad Restaurants

2020 ◽  
Vol 15 (2) ◽  
pp. 4-8
Author(s):  
Ilham Abid Fathi
Keyword(s):  
Listeria Monocytogenes ◽  
International Standards ◽  
Listeria Innocua ◽  
Health Conditions ◽  
Routine Examination ◽  
Listeria Ivanovii ◽  
International Standards Organization ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri ◽  
Different Levels

Background: Listeria monocytogenes, a member of the genus Listeria, is widely distributed in agricultural environments, such as soil, manure and water. The genus of Listeria bacteria is about 15-17 species. It is a pathogenic bacterium that can cause a rare but dangerous infection called listeriosis. Objectives: Studying the rate of salads contaminated with Listeria bacteria. and Listeria monocytogenes according to International, Arabic and Iraqi specifications and finding the correlation between commitments of restaurants to standard health conditions with contamination with these bacteria Methods: The study included 152 samples of salads taken from 39 restaurants chosen randomly and of different levels and places in Baghdad from the period between 1/9/2014 to 20/1/2015. The laboratory tests were carried out on samples based on internationally approved methods in addition to methods of the International Standards Organization. Results: The study revealed that 23 samples (15.13%) from the 152 samples taken from the restaurants were contaminated with Listeria species. of these, 3 (2%) were contaminated with Listeria monocytogenes and 20 (13.2%) were contaminated with other types of different and non-pathogenic Listeria as follows; (Listeria welshimeri, Listeria seeligeri, Listeria ivanovii, Listeria grayi, Listeria innocua) with the following prevalence (7(4.6%), 6(3.9%), 3(2%), 3(2%), 1 (0.7%) respectively). Conclusions: Contamination of salads taken from restaurants with Listeria bacteria is not uncommon.  This indicates that routine examination is necessary and should be added to the Iraqi standard for salads.  

Listeria monocytogenes in Raw Milk: Detection, Incidence, and Pathogenicity

1987 ◽  
Vol 50 (3) ◽  
pp. 188-192 ◽  
Author(s):  
J. LOVETT ◽  
D. W. FRANCIS ◽  
J. M. HUNT
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Sampling Period ◽  
Raw Milk ◽  
The United States ◽  
Isolation Method ◽  
Listeria Species ◽  
Adult Mice ◽  
Listeria Ivanovii

To determine the incidence of Listeria monocytogenes in raw milk, an isolation method was evaluated and used to analyze milk from three areas of the United States. The incidence varied by area from 0% in California to 7% in Massachusetts, with an overall incidence of 4.2%. The highest incidence found in any area during a single sampling period was 12% in Massachusetts in March 1985. During that same sampling, the incidence for all Listeria species was 26%. Of the 27 L. monocytogenes strains isolated during the survey, 25 were pathogenic in adult mice. One of three Listeria ivanovii isolated was pathogenic. No other isolates demonstrated pathogenicity.

Occurrence of Listeria monocytogenes in Milk and Dairy Products Produced or Imported Into Morocco

1993 ◽  
Vol 56 (3) ◽  
pp. 256-259 ◽  
Author(s):  
A. EL MARRAKCHI ◽  
A. HAMAMA ◽  
F. EL OTHMANI
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Listeria Innocua ◽  
Pasteurized Milk ◽  
Listeria Species ◽  
Fresh Cheese ◽  
Milk And Dairy Products

Examination of 227 samples of milk and dairy products for Listeria monocytogenes showed that raw milk and some Moroccan traditionally made dairy products such as Iben and raib (fermented milks) and jben (fresh cheese) were contaminated with this pathogen. L. monocytogenes was the only Listeria species isolated except in one case in which it was associated with Listeria innocua. Pasteurized milk, fresh cream, and fresh and ripened cheeses (industrially made) were free from L. monocytogenes.

Listeria monocytogenes in the Irish Dairy Farm Environment

2009 ◽  
Vol 72 (7) ◽  
pp. 1450-1456 ◽  
Author(s):  
EDWARD FOX ◽  
TOM O'MAHONY ◽  
MARIE CLANCY ◽  
RITA DEMPSEY ◽  
MARTINA O'BRIEN ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Chain ◽  
Foodborne Pathogen ◽  
Dairy Farm ◽  
Raw Milk ◽  
Hygiene Practices ◽  
Unpasteurized Milk ◽  
Cheese Industry ◽  
Chromogenic Agar ◽  
Pcr Targeting

Listeria monocytogenes is a potentially lethal foodborne pathogen commonly found in the environment. European Union hygiene legislation places responsibility for safety on primary production facilities, including farms, as part of a policy to introduce traceability throughout the food chain. This study aimed to determine the occurrence of L. monocytogenes in the Irish dairy farm environment and in particular the milking facility. Two hundred ninety-eight environmental samples were collected from 16 farms in the southern region of Ireland. A number of farms within the group supply raw milk to the unpasteurized milk cheese industry. The samples taken included cow feces, milk, silage, soil, water, etc. Samples were enriched in Listeria enrichment broth and incubated for 48 h, followed by plating on chromogenic agar Listeria Ottavani & Agosti and further incubation of the plates for 24 to 48 h. Presumptive L. monocytogenes isolates were purified and confirmed by PCR targeting the hly gene. Overall, 19% of the samples (57 of 298) were positive for L. monocytogenes. These were serotyped using conventional and PCR methods; serotypes 1/2a, 1/2b, and 4b made up 78% of the typeable isolates. A correlation was found between the level of hygiene standards on the farm and the occurrence of L. monocytogenes. There was little difference in the occurrence of L. monocytogenes between farms supplying milk to the unpasteurized milk cheese industry and those supplying milk for processing. This study demonstrates the prevalence of L. monocytogenes in the dairy farm environment and the need for good hygiene practices to prevent its entry into the food chain.

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