scholarly journals GzRUM1, Encoding an Ortholog of Human Retinoblastoma Binding Protein 2, is Required for Ascospore Development in Gibberella zeae

2011 ◽  
Vol 27 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Hee-Kyoung Kim ◽  
Yin-Won Lee ◽  
Sung-Hwan Yun
Keyword(s):  
Binding Protein ◽  
Gibberella Zeae ◽  
Ascospore Development ◽  
Human Retinoblastoma

scholarly journals The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum)

2007 ◽  
Vol 7 (2) ◽  
pp. 415-424 ◽  
Author(s):  
Heather E. Hallen ◽  
Frances Trail
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Mycelial Growth ◽  
Transcript Abundance ◽  
Gene Replacement ◽  
Gibberella Zeae ◽  
Transcriptional Analysis ◽  
Saprobic Fungi ◽  
Ascospore Discharge ◽  
Ascospore Development

ABSTRACT Cch1, a putative voltage-gated calcium ion channel, was investigated for its role in ascus development in Gibberella zeae. Gene replacement mutants of CCH1 were generated and found to have asci which did not forcibly discharge spores, although morphologically ascus and ascospore development in the majority of asci appeared normal. Additionally, mycelial growth was significantly slower, and sexual development was slightly delayed in the mutant; mutant mycelia showed a distinctive fluffy morphology, and no cirrhi were produced. Wheat infected with Δcch1 mutants developed symptoms comparable to wheat infected with the wild type; however, the mutants showed a reduced ability to protect the infected stalk from colonization by saprobic fungi. Transcriptional analysis of gene expression in mutants using the Affymetrix Fusarium microarray showed 2,449 genes with significant, twofold or greater, changes in transcript abundance across a developmental series. This work extends the role of CCH1 to forcible spore discharge in G. zeae and suggests that this channel has subtle effects on growth and development.

scholarly journals Human Retinoblastoma Binding Protein 9, a Serine Hydrolase Implicated in Pancreatic Cancers

2012 ◽  
Vol 19 (2) ◽  
pp. 194-197 ◽  
Author(s):  
Sergey M. Vorobiev ◽  
Yuanpeng Janet Huang ◽  
Jayaraman Seetharaman ◽  
Rong Xiao ◽  
Thomas B. Acton ◽  
...  
Keyword(s):  
Binding Protein ◽  
Serine Hydrolase ◽  
Pancreatic Cancers ◽  
Human Retinoblastoma

scholarly journals Isolation and chromosomal localization of a new human retinoblastoma binding protein 2 homologue 1a (RBBP2H1A)

2000 ◽  
Vol 8 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Vladimir Kashuba ◽  
Alexei Protopopov ◽  
Raf Podowski ◽  
Rinat Gizatullin ◽  
Jingfeng Li ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Binding Protein ◽  
Human Retinoblastoma

scholarly journals Crystal structure of human retinoblastoma binding protein 9

2009 ◽  
Vol 74 (2) ◽  
pp. 526-529 ◽  
Author(s):  
Sergey M. Vorobiev ◽  
Min Su ◽  
Jayaraman Seetharaman ◽  
Yuanpeng Janet Huang ◽  
Chen X. Chen ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Binding Protein ◽  
Human Retinoblastoma

scholarly journals Mid1, a Mechanosensitive Calcium Ion Channel, Affects Growth, Development, and Ascospore Discharge in the Filamentous Fungus Gibberella zeae

2011 ◽  
Vol 10 (6) ◽  
pp. 832-841 ◽  
Author(s):  
Brad Cavinder ◽  
Ahmed Hamam ◽  
Roger R. Lew ◽  
Frances Trail
Keyword(s):  
Ion Channel ◽  
Calcium Ion ◽  
Vegetative Growth ◽  
Gibberella Zeae ◽  
Calcium Flux ◽  
Wild Type ◽  
Content Type ◽  
Ascospore Discharge ◽  
Ascospore Development

ABSTRACT The role of Mid1, a stretch-activated ion channel capable of being permeated by calcium, in ascospore development and forcible discharge from asci was examined in the pathogenic fungus Gibberella zeae (anamorph Fusarium graminearum ). The Δ mid1 mutants exhibited a >12-fold reduction in ascospore discharge activity and produced predominately abnormal two-celled ascospores with constricted and fragile septae. The vegetative growth rate of the mutants was ∼50% of the wild-type rate, and production of macroconidia was >10-fold lower than in the wild type. To better understand the role of calcium flux, Δ mid1 Δ cch1 double mutants were also examined, as Cch1, an L-type calcium ion channel, is associated with Mid1 in Saccharomyces cerevisiae . The phenotype of the Δ mid1 Δ cch1 double mutants was similar to but more severe than the phenotype of the Δ mid1 mutants for all categories. Potential and current-voltage measurements were taken in the vegetative hyphae of the Δ mid1 and Δ cch1 mutants and the wild type, and the measurements for all three strains were remarkably similar, indicating that neither protein contributes significantly to the overall electrical properties of the plasma membrane. Pathogenicity of the Δ mid1 and Δ mid1 Δ cch1 mutants on the host (wheat) was not affected by the mutations. Exogenous calcium supplementation partially restored the ascospore discharge and vegetative growth defects for all mutants, but abnormal ascospores were still produced. These results extend the known roles of Mid1 to ascospore development and forcible discharge. However, Neurospora crassa Δ mid1 mutants were also examined and did not exhibit defects in ascospore development or in ascospore discharge. In comparison to ion channels in other ascomycetes, Mid1 shows remarkable adaptability of roles, particularly with regard to niche-specific adaptation.

scholarly journals Intracellular Siderophores Are Essential for Ascomycete Sexual Development in Heterothallic Cochliobolus heterostrophus and Homothallic Gibberella zeae

2007 ◽  
Vol 6 (8) ◽  
pp. 1339-1353 ◽  
Author(s):  
Shinichi Oide ◽  
Stuart B. Krasnoff ◽  
Donna M. Gibson ◽  
B. Gillian Turgeon
Keyword(s):  
Sexual Development ◽  
Gibberella Zeae ◽  
Cochliobolus Heterostrophus ◽  
Fungal Development ◽  
Exogenous Application ◽  
Functional Conservation ◽  
Peptide Synthetase ◽  
Ascospore Development ◽  
Mutant Strains ◽  
Biochemical Analyses

ABSTRACT Connections between fungal development and secondary metabolism have been reported previously, but as yet, no comprehensive analysis of a family of secondary metabolites and their possible role in fungal development has been reported. In the present study, mutant strains of the heterothallic ascomycete Cochliobolus heterostrophus, each lacking one of 12 genes (NPS1 to NPS12) encoding a nonribosomal peptide synthetase (NRPS), were examined for a role in sexual development. One type of strain (Δnps2) was defective in ascus/ascospore development in homozygous Δnps2 crosses. Homozygous crosses of the remaining 11 Δnps strains showed wild-type (WT) fertility. Phylogenetic, expression, and biochemical analyses demonstrated that the NRPS encoded by NPS2 is responsible for the biosynthesis of ferricrocin, the intracellular siderophore of C. heterostrophus. Functional conservation of NPS2 in both heterothallic C. heterostrophus and the unrelated homothallic ascomycete Gibberella zeae was demonstrated. G. zeae Δnps2 strains are concomitantly defective in intracellular siderophore (ferricrocin) biosynthesis and sexual development. Exogenous application of iron partially restored fertility to C. heterostrophus and G. zeae Δnps2 strains, demonstrating that abnormal sexual development of Δnps2 strains is at least partly due to their iron deficiency. Exogenous application of the natural siderophore ferricrocin to C. heterostrophus and G. zeae Δnps2 strains restored WT fertility. NPS1, a G. zeae NPS gene that groups phylogenetically with NPS2, does not play a role in sexual development. Overall, these data demonstrate that iron and intracellular siderophores are essential for successful sexual development of the heterothallic ascomycete C. heterostrophus and the homothallic ascomycete G. zeae.

A Screen for New Trithorax Group Genes Identified little imaginal discs, the Drosophila melanogaster Homologue of Human Retinoblastoma Binding Protein 2

Genetics ◽  
2000 ◽  
Vol 156 (2) ◽  
pp. 645-663 ◽  
Author(s):  
John J Gildea ◽  
Rocio Lopez ◽  
Allen Shearn
Keyword(s):  
Expression Pattern ◽  
Binding Protein ◽  
Imaginal Discs ◽  
Polycomb Group ◽  
Homeotic Genes ◽  
New Members ◽  
Drosophila Development ◽  
Trithorax Group ◽  
Human Retinoblastoma ◽  
Conserved Genes

Abstract The proteins encoded by two groups of conserved genes, the Polycomb and trithorax groups, have been proposed to maintain, at the level of chromatin structure, the expression pattern of homeotic genes during Drosophila development. To identify new members of the trithorax group, we screened a collection of deficiencies for intergenic noncomplementation with a mutation in ash1, a trithorax group gene. Five of the noncomplementing deletions uncover genes previously classified as members of the Polycomb group. This evidence suggests that there are actually three groups of genes that maintain the expression pattern of homeotic genes during Drosophila development. The products of the third group appear to be required to maintain chromatin in both transcriptionally inactive and active states. Six of the noncomplementing deficiencies uncover previously unidentified trithorax group genes. One of these deficiencies removes 25D2-3 to 26B2-5. Within this region, there are two, allelic, lethal P-insertion mutations that identify one of these new trithorax group genes. The gene has been called little imaginal discs based on the phenotype of mutant larvae. The protein encoded by the little imaginal discs gene is the Drosophila homologue of human retinoblastoma binding protein 2.

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