Preliminary Study On Latent Membrane Protein-1 (LMP-1) And Bcl-2 Associated X (BAX) In Exosomes Of Patients With Nasopharingeal Cancer In NTB Provincial Hospital

Nasopharyngeal cancer is one of the most common types of cancer in Indonesia, especially head and neck cancer. One of the etiology of this cancer is infection with Epstein-Barr virus. Early diagnosis is difficult to establish because the early signs and symptoms of nasopharyngeal carcinoma are not specific. Examination of latent membrane protein-1 (LMP1) oncogene expression and BCL2-associated X (BAX) gene expression proved to be useful in the identification of nasopharyngeal cancer. LMP1 oncogene is an oncogene that functions as a tumor necrosis factor receptor (TNFR), so that apoptosis does not occur and promotes invasion and metastasis. The BAX gene is a pro-apoptotic gene that inhibits cancer development, but its regulation will be downregulated by LMP1 because LMP can protect B-cells from apoptosis.

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The Epstein-Barr virus oncogene product latent membrane protein 1 engages the tumor necrosis factor receptor-associated death domain protein to mediate B lymphocyte growth transformation and activate NF- B

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.94.23.12592 ◽

1997 ◽

Vol 94 (23) ◽

pp. 12592-12597 ◽

Cited By ~ 276

Author(s):

K. M. Izumi ◽

E. D. Kieff

Keyword(s):

Tumor Necrosis ◽

Epstein Barr Virus ◽

B Lymphocyte ◽

Tumor Necrosis Factor Receptor ◽

Latent Membrane Protein ◽

Latent Membrane Protein 1 ◽

Death Domain ◽

Barr Virus ◽

Epstein Barr ◽

Necrosis Factor

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Upregulation of special AT-rich-binding protein 1 by Epstein–Barr virus latent membrane protein 1 in human nasopharyngeal cells and nasopharyngeal cancer

Journal of General Virology ◽

10.1099/vir.0.046243-0 ◽

2013 ◽

Vol 94 (3) ◽

pp. 507-513 ◽

Cited By ~ 15

Author(s):

Kazuhira Endo ◽

Julia Shackelford ◽

Mitsuharu Aga ◽

Tomokazu Yoshizaki ◽

Joseph S. Pagano

Keyword(s):

Membrane Protein ◽

Epstein Barr Virus ◽

Binding Protein ◽

Nasopharyngeal Cancer ◽

Latent Membrane Protein ◽

Latent Membrane Protein 1 ◽

Tissue Samples ◽

Multifunctional Protein ◽

Barr Virus ◽

Epstein Barr

A global regulator of chromatin remodelling and gene expression, special AT-rich-binding protein 1 (SATB1) has been implicated in promotion of growth and metastasis of a number of cancers. Here, we demonstrate that the principal oncogene of Epstein–Barr virus (EBV), latent membrane protein 1 (LMP1) upregulates SATB1 RNA and protein expression in human nasopharyngeal cell lines. Silencing of endogenously expressed SATB1 with specific short hairpin RNA decreases cell proliferation and resistance to apoptosis induced by growth factor withdrawal. Additionally, we provide evidence that LMP1-mediated expression of Survivin, a multifunctional protein involved in promoting cell growth and survival, is mediated at least in part by SATB1 in human nasopharyngeal cells. Finally, we show that SATB1 protein levels are elevated in tissue samples from patients with nasopharyngeal carcinoma (NPC), and are directly correlated with the expression of LMP1. Taken together, our results suggest that SATB1 functions as a pro-metastatic effector of LMP1 signalling in EBV-positive NPC.

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Expression of tumor necrosis factor receptor-associated factor 1 in nasopharyngeal carcinoma: Possible upregulation by Epstein-Barr virus latent membrane protein 1

International Journal of Cancer ◽

10.1002/ijc.20367 ◽

2004 ◽

Vol 112 (2) ◽

pp. 265-272 ◽

Cited By ~ 8

Author(s):

Gabriele Siegler ◽

Birgit Meyer ◽

Chris Dawson ◽

Elena Brachtel ◽

Jochen Lennerz ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Tumor Necrosis ◽

Epstein Barr Virus ◽

Tumor Necrosis Factor Receptor ◽

Latent Membrane Protein ◽

Latent Membrane Protein 1 ◽

Barr Virus ◽

Epstein Barr ◽

Virus Latent Membrane Protein ◽

Necrosis Factor

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Sequence Variations of Epstein-Barr Virus-Encoded Small Noncoding RNA and Latent Membrane Protein 1 in Hematologic Tumors in Northern China

Intervirology ◽

10.1159/000510398 ◽

2021 ◽

Vol 64 (2) ◽

pp. 69-80

Author(s):

Hai-Yu Wang ◽

Lingling Sun ◽

Ping Li ◽

Wen Liu ◽

Zhong-Guang Zhang ◽

...

Keyword(s):

Membrane Protein ◽

Nested Pcr ◽

Noncoding Rna ◽

Epstein Barr Virus ◽

Northern China ◽

Latent Membrane Protein ◽

Latent Membrane Protein 1 ◽

Small Noncoding Rna ◽

Barr Virus ◽

Epstein Barr

Objective: To investigate the relationship between hematologic tumors and Epstein-Barr virus (EBV)-encoded small noncoding RNA (EBER) variations as well as latent membrane protein 1 (LMP1) variations. Methods: Patients with leukemia and myelodysplastic syndrome (MDS) were selected as subjects. Genotypes 1/2 and genotypes F/f were analyzed using the nested PCR technology, while EBER and LMP1 subtypes were analyzed by the nested PCR and DNA sequencing. Results: Type 1 was more dominant than type 2, found in 59 out of 82 (72%) leukemia and in 31 out of 35 (88.6%) MDS, while type F was more prevalent than type f in leukemia (83/85, 97.6%) and MDS (29/31, 93.5%) samples. The distribution of EBV genotypes 1/2 was not significantly different among leukemia, MDS, and healthy donor groups, neither was that of EBV genotypes F/f. EB-6m prototype was the dominant subtype of EBER in leukemia and MDS (73.2% [30/41] and 83.3% [10/12], respectively). The frequency of EB-6m was lower than that of healthy people (96.7%, 89/92), and the difference was significant (p < 0.05). China 1 subtype was the dominant subtype of LMP1 in leukemia and MDS (70% [28/40] and 90% [9/10], respectively), and there was no significant difference in the distribution of LMP1 subtypes among the 3 groups (p > 0.05). Conclusion: The distribution of EBV 1/2, F/f, EBER, and LMP1 subtypes in leukemia and MDS was similar to that in the background population in Northern China, which means that these subtypes may be rather region-restricted but not associated with leukemia and MDS pathogenesis.

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