Imunohistochemical and pathomorphological studies of chronic granulomatous enteritis (Johne's disease) in bovine

ABSTRACT Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.

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Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds

Veterinary Medicine International ◽

10.1155/2012/145318 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Bárbara Fernández ◽

Liliana Rosa Gilardoni ◽

Ana Jolly ◽

Silvia Beatriz Colavecchia ◽

Fernando Alberto Paolicchi ◽

...

Keyword(s):

Operating Characteristic ◽

High Sensitivity ◽

Roc Curves ◽

Disease Diagnosis ◽

Johne's Disease ◽

Johne’S Disease ◽

Infected Cattle ◽

Serological Tests ◽

Igg Isotypes ◽

Granulomatous Enteritis

Johne's Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected withMapin different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n=30), subclinically infected (n=26), clinically infected (n=14), and healthy controls (n=38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account allMap-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification ofMap-infected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne's Disease diagnosis should be further evaluated.

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Apoptosis levels in bovine Johne’s disease ileal lesions and association with bacterial numbers

Veterinary Pathology ◽

10.1177/03009858211025790 ◽

2021 ◽

pp. 030098582110257

Author(s):

Amalia Naranjo- Lucena ◽

Laura Garza-Cuartero ◽

Conor McAloon ◽

Grace Mulcahy ◽

Annetta Zintl ◽

...

Keyword(s):

Cell Types ◽

Treg Cells ◽

Johne's Disease ◽

Johne’S Disease ◽

Phagocytic Cells ◽

Tissue Sections ◽

Lesion Type ◽

Mycobacterium Avium Subsp Paratuberculosis ◽

Ileal Tissue ◽

Granulomatous Enteritis

Johne’s disease (JD) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP). While it is generally accepted that MAP employs immune subversion mechanisms, aspects of the host-pathogen relationship are not fully understood. We sampled 3 ileal tissue sections from 17 naturally infected cattle ( n = 51 sections) to analyze differences in cell types, apoptosis, and phagocytic cells. Diffuse multibacillary (DM) was the most common lesion type ( n = 17) followed by diffuse intermediate (DI; n = 15). DM lesions had significantly greater proportion of Treg cells (CD3+ FoxP3+) relative to all CD3+ T cells as compared to DI forms ( P < .05). CD68+ individual cell size was significantly smaller in DM than in diffuse lymphocytic (DL) forms ( P < .05). Area of caspase-3 positivity (apoptosis) was greater in DM lesions than DL ( P < .05) and DI ( P < .0001), and was linked to higher numbers of MAP within the macrophage.

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