Assessment of protective effect of ascorbic acid in cisplatin ototoxicity on guinea pigs with electrophysiological tests and ultrastructural study: A Preliminary Study

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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Guinea pigs possess a highly mutated gene for L-gulono-gamma-lactone oxidase, the key enzyme for L-ascorbic acid biosynthesis missing in this species.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)36707-9 ◽

1992 ◽

Vol 267 (30) ◽

pp. 21967-21972 ◽

Cited By ~ 2

Author(s):

M Nishikimi ◽

T Kawai ◽

K Yagi

Keyword(s):

Ascorbic Acid ◽

Guinea Pigs ◽

Acid Biosynthesis ◽

Key Enzyme ◽

Ascorbic Acid Biosynthesis

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PARALLEL STUDIES OF COMPLEMENT AND BLOOD COAGULATION IX. THE EFFECT OF METHIONINE, CHOLINE, AND CYSTINE ON THE CHANGES INDUCED BY ETHIONINE

Canadian Journal of Medical Sciences ◽

10.1139/cjms53-036 ◽

1953 ◽

Vol 31 (4) ◽

pp. 343-357

Author(s):

Christine E. Rice ◽

Paul Boulanger ◽

P. J. G. Plummer ◽

E. Annau

Keyword(s):

Fatty Liver ◽

Protective Effect ◽

Blood Coagulation ◽

Guinea Pigs ◽

Acinar Cells ◽

Complement Components ◽

Fatty Livers

Fatty livers were produced in guinea pigs by the repeated feeding or injection of ethionine; the acinar cells of the pancreas were also affected in some of the animals. Marked changes in plasma coagulability always occurred as well as a definite reduction in complement titer in which two or more of the major complement components were involved. Methionine displayed some protective effect against the fatty liver induced by the ethionine and was partially effective in controlling the coagulative changes and the decline in complement titer. In some animals, choline likewise ameliorated these conditions, whereas cystine tended to aggravate them. A combination of cystine and choline was more effective than choline alone.

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