Factors Influencing the Sperm Motility and Viability Parameters of Post-Thawed Semen of Tunisian Arab Stallions

This study aimed to evaluate whether sperm motility (MOT) in dog semen is influenced by dog age, breed, or number of sperm collections. The study group consisted of four Shih Tzus and five Border Collies, aged 1–8 years. Sperm was collected from each dog once every seven days, for a total of six weeks. Sperm motility did not differ (P > 0.05) based on age and breed. Mean MOT at the first collection (67.4% ± 6.9%) was lower (P < 0.05) than that at the third, fifth, and sixth collections (86.2%; 88.9%; 89.1% ± 6.9%, respectively). Increased MOT was observed after the third collection, demonstrating a positive correlation with the number of sperm collections and sperm motility (P< 0.05). Accordingly, our results indicate that sperm processing efficiency is increased after the collection of three ejaculates.

Download Full-text

Slow cooling prevents cold-induced damage to sperm motility and acrosomal integrity in the black-footed ferret (Mustela nigripes)

Reproduction Fertility and Development ◽

10.1071/rd06096 ◽

2007 ◽

Vol 19 (5) ◽

pp. 652 ◽

Cited By ~ 18

Author(s):

R. M. Santymire ◽

P. E. Marinari ◽

J. S. Kreeger ◽

D. E. Wildt ◽

J. G. Howard

Keyword(s):

Sperm Motility ◽

Seminal Plasma ◽

Artificial Insemination ◽

Sperm Cryopreservation ◽

Slow Cooling ◽

Rapid Cooling ◽

Mustela Nigripes ◽

Factors Influencing ◽

Rate Of Cooling ◽

Acrosomal Integrity

The endangered black-footed ferret (Mustela nigripes) has benefited from artificial insemination; however, improved sperm cryopreservation protocols are still needed. The present study focused on identifying factors influencing gamete survival during processing before cryopreservation, including: (1) the presence or absence of seminal plasma; (2) temperature (25°C v. 37°C); (3) type of medium (Ham’s F10 medium v. TEST yolk buffer [TYB]); (4) cooling rate (slow, rapid and ultra-rapid); and (5) the presence or absence of glycerol. Seminal plasma did not compromise (P > 0.05) sperm motility or acrosomal integrity. Sperm motility traits were maintained longer (P < 0.05) at 25°C than at 37°C in Ham’s or TYB, but temperature did not affect (P > 0.05) acrosomal integrity. Overall, TYB maintained optimal (P < 0.05) sperm motility compared with Ham’s medium, but Ham’s medium maintained more (P < 0.05) intact acrosomes than TYB. Slow cooling (0.2°C min–1) was optimal (P < 0.05) compared to rapid cooling (1°C min–1), and ultra-rapid cooling (9°C min–1) was found to be highly detrimental (P < 0.05). Results obtained in TYB with 0% or 4% glycerol were comparable (P > 0.05), indicating that 4% glycerol was non-toxic to ferret sperm; however, glycerol failed to ameliorate the detrimental effects of either rapid or ultra-rapid cooling. The results of the present study demonstrate that the damage observed to black-footed ferret spermatozoa is derived largely from the rate of cooling.

Download Full-text

Absence of antisperm antibodies and factors influencing sperm motility in the cul-de-sac fluid of women with endometriosis

Fertility and Sterility ◽

10.1016/s0015-0282(16)53294-4 ◽

1990 ◽

Vol 53 (2) ◽

pp. 351-353 ◽

Cited By ~ 7

Author(s):

Richard E. Leach ◽

Brian W. Arneson ◽

G. David Ball ◽

Steven J. Ory

Keyword(s):

Sperm Motility ◽

Antisperm Antibodies ◽

Factors Influencing

Download Full-text

Factors influencing maintenance of sperm motility during in vitro processing

Fertility and Sterility ◽

10.1016/s0015-0282(16)59537-5 ◽

1987 ◽

Vol 48 (5) ◽

pp. 816-820 ◽

Cited By ~ 15

Author(s):

Dominique de Ziegler ◽

Marcelle I. Cedars ◽

Fredesminda Hamilton ◽

Tracy Moreno ◽

David R. Meldrum

Keyword(s):

Sperm Motility ◽

Factors Influencing ◽

In Vitro Processing

Download Full-text

Factors influencing maintenance of sperm motility during in vitro processing

International Journal of Gynecology & Obstetrics ◽

10.1016/0020-7292(88)90048-3 ◽

1988 ◽

Vol 27 (2) ◽

pp. 312-313

Author(s):

D De Ziegler ◽

MI Cedars ◽

F Hamilton

Keyword(s):

Sperm Motility ◽

Factors Influencing ◽

In Vitro Processing

Download Full-text

Arrangement of Mitochondria in Spermatozoa of the Mexican Axolotl, Ambystoma Mexicanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007357x ◽

1973 ◽

Vol 31 ◽

pp. 626-627

Author(s):

Ezzatollah Keyhani ◽

Larry F. Lemanski ◽

Sharon L. Lemanski

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Substrate Oxidation ◽

Ambystoma Mexicanum ◽

Axial Filament ◽

Mexican Axolotl ◽

Middle Piece

Energy for sperm motility is provided by both glycolytic and respiratory pathways. Mitochondria are involved in the latter pathway and conserve energy of substrate oxidation by coupling to phosphorylation. During spermatogenesis, the mitochondria undergo extensive transformation which in many species leads to the formation of a nebemkem. The nebemkem subsequently forms into a helix around the axial filament complex in the middle piece of spermatozoa.Immature spermatozoa of axolotls contain numerous small spherical mitochondria which are randomly distributed throughout the cytoplasm (Fig. 1). As maturation progresses, the mitochondria appear to migrate to the middle piece region where they become tightly packed to form a crystalline-like sheath. The cytoplasm in this region is no longer abundant (Fig. 2) and the plasma membrane is now closely apposed to the outside of the mitochondrial layer.

Download Full-text

Factors influencing adhesive bonding at the bone/implant interface

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130201 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1114-1115

Author(s):

Julie A. Martini ◽

Robert H. Doremus

Keyword(s):

Electron Microscopy ◽

Adhesive Bonding ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Bone Implant ◽

Factors Influencing ◽

Lr White ◽

Transmission Electron ◽

New Zealand White Rabbits ◽

Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

Download Full-text

Factors Influencing Electrophysiologic Responsivity in Normal Adults

Journal of Speech and Hearing Research ◽

10.1044/jshr.0804.323 ◽

1965 ◽

Vol 8 (4) ◽

pp. 323-347

Author(s):

Robert Goldstein ◽

Benjamin RosenblÜt

Keyword(s):

Conditioned Stimulus ◽

White Light ◽

Pure Tone ◽

Alpha Rhythm ◽

White Women ◽

White Men ◽

Inverse Relation ◽

Factors Influencing ◽

Electrodermal Responses ◽

Normal Adults

Electrodermal and electroencephalic responsivity to sound and to light was studied in 96 normal-hearing adults in three separate sessions. The subjects were subdivided into equal groups of white men, white women, colored men, and colored women. A 1 000 cps pure tone was the conditioned stimulus in two sessions and white light was used in a third session. Heat was the unconditioned stimulus in all sessions. Previously, an inverse relation had been found in white men between the prominence of alpha rhythm in the EEG and the ease with which electrodermal responses could be elicited. This relation did not hold true for white women. The main purpose of the present study was to answer the following questions: (1) are the previous findings on white subjects applicable to colored subjects? (2) are subjects who are most (or least) responsive electrophysiologically on one day equally responsive (or unresponsive) on another day? and (3) are subjects who are most (or least) responsive to sound equally responsive (or unresponsive) to light? In general, each question was answered affirmatively. Other factors influencing responsivity were also studied.

Download Full-text