The long-term effects of high nitrogen supply on the growth and partitioning of biomass in a common sedge species, Carex rostrata Stokes, were studied in a greenhouse experiment. Special attention was paid to free amino acids and soluble sugars, representing biochemically available fractions of nitrogen and carbon, respectively, in the tissues of Carex. Plants were grown in peat in buckets, and nitrogen was added as ammonium nitrate (2, 5, and 10 g N m-2 year-1) five times during two growing seasons. Changes in biomass allocation patterns became evident towards the end of the second growing season. The biomass of shoots was highest in the high-N treatment, resulting in a high ratio of aboveground to belowground biomass. The high biomass of shoots was due to both the high density of current-year shoots and later senescence in the high-N treatment. No differences were observed in the belowground biomasses. Changes in allocation patterns were accompanied by changes in the soluble fractions of carbon and nitrogen. The concentration of free amino acids (FAA) was significantly higher (both shoots and roots) and the concentration of total nonstructural carbohydrates (TNC) lower (roots only) in the high-N treatment. The concentration of total nitrogen also increased with increasing supply of nitrogen. The results indicate that a high long-term supply of nitrogen may shift the internal carbon to nitrogen balance of Carex towards higher availability of nitrogen. Compared with the carbon to nitrogen ratio, the TNC:FAA ratio seems to be a better indicator of the internal carbon to nitrogen balance. A low TNC:FAA ratio may enhance the allocation of biomass to shoots and also increase the density of shoots.Key words: amino acids, biomass partitioning, carbon, Carex, nitrogen, sugars.