Study on amino acid composition of HSP70 and the level of plasma free amino acids of workers with long-term exposure to harmful factors

1998 ◽  
Vol 18 (4) ◽  
pp. 204-207 ◽  
Author(s):  
Wu Tangchun ◽  
Wu Yang ◽  
Yuan Ye ◽  
He Hanzhen ◽  
Zhang Guogao
Author(s):  
Yu. E. Razvodovsky ◽  
V. Yu. Smirnov ◽  
I. N. Semenenya

The effects of complex compositions, containing branched-chain amino acids (BCAA), taurine and tryptophan, on the pool of free amino acids in the liver of rats were studied under the conditions of subchronic alcohol intoxication (SHAI). It was established that SHAI led to the decreased levels of treonine, lysine, oxyproline, arginine, b-alanine, as well as the depletion of the pool of irreplaceable amino acids in the liver of rats. Administration of the composition of BCAA and taurine was found to normalize the ratio of replaceable irreplaceable amino acids, the ratio of glycogenic and ketogenic amino acids, to activate the reaction of nitrogen utilization, and to increase Fisher’s index. The effects of the composition, containing BCAA, taurine and tryptophan, were similar to those of amino acid composition that did not contain tryptophan.


1959 ◽  
Vol 12 (2) ◽  
pp. 160 ◽  
Author(s):  
JR Freney ◽  
CC Delwiche ◽  
CM Johnson

Data are presented on the amino acid composition of two varieties of Brassica oleracea as influenced by supply of chloride ion to the plant.


1969 ◽  
Vol 101 (11) ◽  
pp. 1207-1213 ◽  
Author(s):  
K. Vick ◽  
W. A. Drew ◽  
J. Young ◽  
E. J. Eisenbraun

AbstractExtracts of free amino acids from six species of ants (Pogonomyrmex barbatus (F. Smith), P. occidentalis (Cresson), Tapinoma sessile (Say), Camponotus pennsylvanicus (DeGeer), Conomyrma pyramica (Roger), and Novomessor cockerelli (E. André) were analyzed by thin layer, one and two dimensional paper chromatography, and an automatic amino acid analyzer to determine the potential use of qualitative and (or) quantitative data of the free amino acid composition of ants in taxonomic studies. The results demonstrate that no significant qualitative differences exist, but that the automatic amino acid analyzer may be used to show significant quantitative differences in the free amino acid composition of the ants studied.


2020 ◽  
Vol 14 (4) ◽  
pp. 80-84
Author(s):  
Sufiya Murtazina ◽  
Liliya Gaffarova ◽  
Malik Murtazin ◽  
Anastasiya Ahrarova

The article presents the results of studying the amino acid composition in the prevailing soils of the region (RT): sod-podzolic, gray forest, brown-gray, leached chernozem. Free and related amino acids are identified, their quantitative indicators are given. A study of the qualitative composition of free amino acids in the soils of the region showed that they are close in the qualitative composition of amino acids, but differ in their quantitative content. The total content of free amino acids is 4-20 mg/kg and increases in the series of soils: sod-medium podzolic, brown-gray, gray forest and leached chernozem. Moreover, there are significant differences in the content of individual groups of free amino acids: in all soils, most of the free amino acids are represented by neutral and basic amino acids (65-80%), much smaller by dicarboxylic (11-29%) and cyclic (5-11%). Easily and hardly hydrolyzable fractions of organic nitrogen are the closest reserves of nitrogen available to plants. Identification of the amino acid composition of these nitrogen fractions showed that the qualitative composition of bound amino acids is almost the same as free. In the studied soils, 19-31% of nitrogen of easily hydrolyzable and 33-60% of nitrogen of hard hydrolyzable fractions are represented by amino acid nitrogen. At the same time, 40-54% of the total amount of nitrogen of amino acids falls on the share of neutral, and 14-28% - on the share of basic amino acids. Their content naturally increases from sod-podzolic to gray soil and to chernozem, both in the composition of easily hydrolyzed and difficultly hydrolyzed fractions, which indicates an improvement in the quality of nitrogen in this direction.


2013 ◽  
Vol 41 (1) ◽  
pp. 69-75
Author(s):  
Ryszard Kosson

The contribution of cotyledons, embryo and testa to the whole seed, was analyzed in the bean cultivar, Wiejska. The total nitrogen content and amino acid composition of morphological parts of the seed were determined. The average amino acid composition of globulins and albumins and the content of free amino acids in seeds of six Polish cultivars were estimated as well. It was found that the embryo contained the highest quantity of total nitrogen and the lowest of protein nitrogen. The exogenous amino acid content in the embryo was higher than in cotyledons and testa. Both albumins and globulins were shown to contain 42% exogenous amino acids. The content of methionine - the first limiting amino acid of bean proteins - did not exceed 0.30% of the total amino acid content in albumins and globulins. Free glutamic and aspartic acids made up more than 60% of the total free amino acids.


2017 ◽  
pp. 67-71
Author(s):  
V. M. Sheybak ◽  
A. Y. Pavliukovets ◽  
V. Yu. Smirnov

Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.


1967 ◽  
Vol 45 (2) ◽  
pp. 225-234 ◽  
Author(s):  
Tsang-Cheng Shao ◽  
D. C. Hill

As previously reported, an increased concentration of plasma free amino acids, particularly lysine and threonine, was found when chicks were fasted for 24 hours. This increase was suppressed where a nonprotein diet was fed. Dextrose as the chief energy source in the nonprotein diet was more effective than shortening or lard in this respect. However, the degree to which the two energy sources differed was less where birds were adapted to the utilization of fat by feeding a diet rich in fat for a considerable time before making the comparison. Insulin administration to fasted chicks was found to lower plasma amino acid levels. The possible influence of interrelationships among dextrose, fat, and insulin on plasma amino acid levels is discussed.


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