FORMULASI SEDIAAN MASKER GEL PEEL-OFF EKSTRAK DAUN CABAI RAWIT DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI Staphylococcus Aureus

Munifatul Lailiyah; Sony Andika Saputra; Jeki Kurniawan

doi:10.55093/herbapharma.v3i2.262

FORMULASI SEDIAAN MASKER GEL PEEL-OFF EKSTRAK DAUN CABAI RAWIT DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI Staphylococcus Aureus

HERBAPHARMA : Journal of Herb Farmacological ◽

10.55093/herbapharma.v3i2.262 ◽

2021 ◽

Vol 3 (2) ◽

pp. 56-69

Author(s):

Munifatul Lailiyah ◽

Sony Andika Saputra ◽

Jeki Kurniawan

Keyword(s):

Staphylococcus Aureus

Daun cabe rawit (Capsicum frustenses L.) dapat digunakan sebagai pengobatan jerawat. Daun cabe rawit telah diketahui mengandung senyawa aktif flavonoid yang memiliki efektivitas sebagai antibakteri. Pada penelitian ini daun cabe rawit dikembangkan menjadi sediaan masker gel peel-off. Penelitian ini bertujuan untuk mengetahui perbedaan karakteristik atau mutu fisik formulasi sediaan masker gel peel-off dari ekstrak etanol daun cabe rawit (Capsicum frustenses L.) dan mengetahui aktivitas antibakteri dari formulasi sediaan masker gel peel-off ekstrak etanol daun cabe rawit (Capsicum frustenses L.) tehadap bakteri Staphylococcus Aureus. Daun cabe rawit di ekstraksi menggunakan pelarut etanol 70 % dengan metode ekstraksi maserasi. Ekstrak yang didapat dibuat sediaan masker gel peel-off dengan variasi konsentrasi yang berbeda yaitu 5 %, 10 %, 15 %. Sediaan masker gel peel-off yang sudah dibuat dilakukan pengujian karakteristik meliputi organoleptis, homogenitas, pH, daya sebar, daya lekat, waktu mengering, iritasi dan uji aktivitas antibakteri. Pengujian aktivitas antibakteri dilakukan dengan menggunakan metode difusi cakram. Hasil penelitian menunjukkan bahwa masker gel peel-off ekstrak etanol daun cabai rawit (Capsicum frustenses L.) memenuhi parameter uji mutu fisik yaitu organoleptik, homogenitas, daya sebar, daya lekat, waktu mengering dan uji aktifitas antibakteri pada konsentrasi I sebesar 4,11 mm, formulasi II dan III masing-masing 5,48 mm dan 7,11 mm.

Download Full-text

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094905 ◽

1972 ◽

Vol 30 ◽

pp. 328-329

Author(s):

Masaatsu Koike ◽

Koichi Nakashima ◽

Kyoko Iida

Keyword(s):

Staphylococcus Aureus ◽

Periodate Oxidation ◽

Early Time ◽

The Other ◽

Penicillin G ◽

Cell Wall Biosynthesis ◽

Septal Wall ◽

Peptidoglycan Biosynthesis ◽

Gram Positive Bacteria ◽

Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Download Full-text

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160443 ◽

1990 ◽

Vol 48 (3) ◽

pp. 578-579

Author(s):

Margaret Hukee

Keyword(s):

Staphylococcus Aureus ◽

Teichoic Acid ◽

Protein Labeling ◽

Minimal Amount ◽

Section Thickness ◽

Double Labeling ◽

Parallel Surfaces ◽

Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

Download Full-text