scholarly journals Induction of Indirect Somatic Embryogenesis on Embryonic Axis of TRI2025 Tea Clone

2018 ◽  
Vol 10 (10) ◽  
pp. 224
Author(s):  
Ratna D. Eskundari ◽  
Taryono Taryono ◽  
Didik Indradewa ◽  
Yekti A. Purwestri
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Light Condition ◽  
Culture Method ◽  
Main Method ◽  
Central Java ◽  
Indirect Somatic Embryogenesis ◽  
Tissue Culture Method ◽  
Vegetative Multiplication

Tea (Camellia sinensis L.) is classified as cross-pollinated crop and vegetative multiplication becomes commercially the main method of propagation with some limitations such as high heterogeneity and poor in survival rate and also in rooting. A proven tissue culture method, somatic embryogenesis, is the only challenging way to meet the needs of tea seedlings in large quantities. The study was conducted with TRI2025 tea clone selected from Polyclonal garden of PT. Pagilaran (Batang, Central Java). The explants were cultured on MS media supplemented with four concentrations of 2,4-D (0, 1, 2, and 5 mg L-1) in two incubation conditions; dark and light. The results showed the only concentration of 2,4-D that can induce somatic embryo was 2 mg L-1 2,4-D in light condition and its percentage was about 5%. Other concentrations of 2,4-D that given for treatments both in two conditions will not induce somatic embryo. This study needs more improvements for getting powerful and efficient of method to get somatic embryo-derived plant and also for futher successful genetic engineering of tea biotechnology.

scholarly journals Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

2016 ◽  
Vol 3 (2) ◽  
pp. 71
Author(s):  
Nur Ajijah ◽  
RR. Sri Hartati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Theobroma Cacao ◽  
Somatic Embryos ◽  
Interaction Effects ◽  
Embryo Formation ◽  
Primary Somatic Embryogenesis ◽  
Primary Somatic Embryos

<p><em>Information on the effect of cytokinins on cacao (</em>Theobroma cacao<em> L.) primary somatic embryogenesis and its interaction with explant types and genotypes is not yet known. This study aimed to evaluate the effect of cytokinins and its interaction with explant types and genotypes on cacao somatic embryogenesis. The study was conducted at tissue culture laboratory of IAARD, Bogor from April until December 2012 and October 2014 until February 2016. Three types of cytokinins i.e. kinetin (0.58, 1.16, and 2.32 </em><em>μ</em><em>M), thidiazuron (0.01, 0.02, and 0.04 </em><em>μ</em><em>M) and benzylaminopurine (0.55, 1.11, and 2.22 </em><em>μ</em><em>M) in combination with 9 </em><em>μ</em><em>M 2,4-D were tested for their effectiveness in inducing somatic embryogenesis from petals and staminoid explants of Cimanggu 1 genotype. Furthermore, three levels of kinetin (0.58, 1.16, and 2.32 </em><em>μ</em><em>M</em><em>) also in combination with 9 </em><em>μ</em><em>M 2,4-D were evaluated for their influences on the somatic embryogenesis from petals and staminoid explants of three cacao genotypes i.e. Sulawesi 02, ICCRI 04 and Cimanggu 3. The result demonstrated that 2.32 </em><em>μ</em><em>M kinetin and staminoids explant were more effective to induce cacao somatic embryogenesis of Cimanggu 1 genotype (7%, 0.23 embryos/explant). Additionally, there were interaction effects between the level of kinetin with explant types and genotype on the percentage of explants forming embryo at 12 weeks after culture. The highest percentage of somatic embryo formation was shown by ICCRI 04 genotype with the use of petals explant and a kinetin level of 1.16 </em><em>μ</em><em>M (31.85%), but not significantly different from the level of kinetin 2.23 </em><em>μ</em><em>M (25.55%). The formation of primary somatic embryos of cacao is largely determined by the type and level of cytokinins, type of explant, and genotype.</em></p>

scholarly journals Rapid Tissue Culture Method for Detection of Mycoplasma hyorhinis

Microbiology ◽  
1982 ◽  
Vol 128 (11) ◽  
pp. 2817-2820
Author(s):  
J. A. Levy ◽  
P. E. Sumner ◽  
L. E. Hooser
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Mycoplasma Hyorhinis ◽  
Tissue Culture Method

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

scholarly journals Estimation of Biocompatibility of High Strength β-Tricalcium Phosphate Ceramics by a Tissue Culture Method

1990 ◽  
Vol 98 (1136) ◽  
pp. 404-407 ◽  
Author(s):  
Motohiro TORIYAMA ◽  
Sukezo KAWAMURA ◽  
Yukari KAWAMOTO ◽  
Takahiro SUZUKI ◽  
Yoshiyuki YOKOGAWA ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Tricalcium Phosphate ◽  
High Strength ◽  
Culture Method ◽  
Tissue Culture Method

scholarly journals Vast propagation of submerged tool species Hydrilla verticillata with tissue culture method

2008 ◽  
Vol 20 (2) ◽  
pp. 215-220
Author(s):  
JIANG Jinhui ◽  
◽  
ZHOU Changfang ◽  
AN Shuqing ◽  
GUAN Baohua ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Hydrilla Verticillata ◽  
Culture Method ◽  
Tissue Culture Method

scholarly journals Plant Regeneration via Somatic Embryogenesis in Larix principis-rupprechtii Mayr

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1335
Author(s):  
Shuaifei Jiang ◽  
Xiaoyi Chen ◽  
Ying Gao ◽  
Ying Cui ◽  
Lisheng Kong ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
High Efficiency ◽  
Germination Rate ◽  
Culture Method ◽  
Culture Conditions ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Embryo Maturation ◽  
Somatic Embryo Maturation

Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) is a native conifer in North China with great economic and ecological values. Somatic embryogenesis (SE) is a powerful tool for the mass clonal propagation in plants. In this study, we described a high-efficiency SE system via indirect pathways and investigated the effect of genotype, culture conditions and phytohormones on SE. Immature zygotic embryos (IZEs) of L. principis-rupprechtii Mayr were used as explant materials. In the induction stage, embryogenic tissues (ETs) were induced on mLV medium supplemented with 2.0 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg L−1 6-benzylaminopurine (6-BA). The initiation frequencies showed significant differences (p < 0.05) among 20 genotypes of open-pollinated mother trees with the highest induction frequency reaching 30%. For tissue proliferation, proliferation in liquid medium was more efficient compared with proliferation in semi-solid medium, providing a multiplication rate of 3.12 in an 8-day subculture period. As a necessary exogenous plant growth regulator (PGR) for somatic embryo maturation in conifers, abscisic acid (ABA) was optimized at 16 mg L−1 in this system. Next, an orthogonal test on osmotic pressure factors showed 50 g L−1 sucrose, 7 g L−1 phytagel and 75 g L−1 polyethylene glycol (PEG) was the optimal combination for somatic embryo maturation in L. principis-rupprechtii Mayr. Moreover, the dispersion culture method provided a more efficient somatic embryo maturation, up to 545 per gram of fresh weight (FW). Finally, 2 g L−1 of active charcoal (AC) was found to increase the somatic embryo germination rate to 63.46%. The improved protocol of SE will serve as a foundation for establishing mass propagation and genetic transformation of L. principis-rupprechtii Mayr.

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