scholarly journals Influence of Drought and Wounding Stress on Soluble Phenols and Proteins in Potato Tubers

2014 ◽  
Vol 3 (3) ◽  
pp. 1 ◽  
Author(s):  
Christina B. Wegener ◽  
Gisela Jansen ◽  
Hans-Ulrich Jurgens
Keyword(s):  
Amino Acids ◽  
Drought Stress ◽  
Water Supply ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers ◽  
Fresh Tissue ◽  
Plant Phenols ◽  
Wounding Stress ◽  
Soluble Phenols

<p>Potato is a valuable source for phytochemicals like vitamins, minerals, plant phenols, amino acids and proteins. However, environmental stress may affect the level of all these components. In this study, two purple breeding clones and one yellow fleshed cultivar (cv.) were assessed in their response to drought and wounding stress in two consecutive years. The plants were grown in the greenhouse under control (sufficient water supply) and with drought stress conditions. After harvest the tubers were analyzed for the content of soluble phenols and proteins in fresh tissue and after wounding. In addition, free amino acids (AAS) and activity of lipid acyl hydrolases (LAH) were assayed in control and drought stressed tubers. The results revealed significant differences in soluble phenols and proteins between genotypes, and that drought stress significantly increased the level of soluble proteins (P &lt; 0.0001) and LAH activity (P &lt; 0.001), but had no significant effect on the concentration of phenols. Moreover, total amounts of free AAS were higher in the drought stress variant. Wounding stress caused a significant increase of soluble phenols in cv. Agave. But, this was less prominent in purple clones which in general had higher contents of phenols. Proteins were also enhanced as a result of wounding, although, the effect of wounding stress on their level was smaller than that of drought stress.<strong></strong></p>

scholarly journals Effect of drought and waterlogging on saccharides and amino acids content in potato tubers  

2021 ◽  
Author(s):  
Matyáš Orsák ◽  
Zora Kotíková ◽  
František Hnilička ◽  
Jaromír Lachman
Keyword(s):  
Amino Acids ◽  
Drought Stress ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers ◽  
High Effect ◽  
Total Free Amino Acids ◽  
Effects Of Drought ◽  
Tyrosine Content ◽  
Total Amino Acids

The study was focused on the effect of drought and waterlogging stresses in two-year pot experiments in the peat substrate on the content of glucose, fructose and sucrose and free amino acids in potato tubers of four cultivars (yellow-fleshed Laura, Marabel, Milva and blue-fleshed Valfi) after 71 days of exposure to stresses conditions (BBCH 909). Drought and waterlogging increased levels of fructose, glucose, and sucrose in three potato cultivars except for cv. Laura. Drought stress increased l-proline (+248.4%), l-hydroxyproline (+135.3%), l-arginine (+29.97%), l-glutamic acid (+29.09%) and l-leucine (+22.58%) contents in all analysed cultivars. Moreover, the high effect of drought stress on an increase of l-phenylalanine, l-histidine, l-threonine, and total free amino acids content of the cvs. Laura, Valfi and Marabel has been observed. A comparison of the effects of drought and waterlogging stresses on the content of total amino acids showed an increase under drought and a decrease under waterlogging conditions. On average, of all cultivars, waterlogging stress caused an increase of l-tyrosine content, whereas drought stress decrease. In addition, drought stress caused a significant increase of l-proline in all cultivars while waterlogging its decrease. Obtained results confirmed different responses of susceptible or resistant cultivars to abiotic stresses.  

scholarly journals Using two plant growth promoting bacteria to sustainably reduce the drought-induced loss in Triticum aestivum yield

2020 ◽  
Vol 12 (2) ◽  
pp. 433-446
Author(s):  
El-Anwar OSMAN ◽  
Wedad A. KASIM ◽  
Nabil A. OMAR ◽  
Samar E. SALAMA
Keyword(s):  
Amino Acids ◽  
Drought Stress ◽  
Plant Growth ◽  
Free Amino Acids ◽  
Free Amino ◽  
Azospirillum Brasilense ◽  
Stenotrophomonas Maltophilia ◽  
Plant Growth Promoting ◽  
Total Free Amino Acids ◽  
Growth Promoting

In a greenhouse experiment, the inoculated and uninoculated grains with Azospirillum brasilense NO40 or Stenotrophomonas maltophilia were sown in unsterilized sandy soil and watered normally till the 8th day. Thereafter, the drought stress was initiated by watering pots once every 10 days while the unstressed pots were irrigated normally once every 5 days. Samples of spikes and dry grains were collected after 120 days from sowing. The results indicated that the inoculated-drought-stressed plants maintained significantly higher values of all of the measured yield parameters, where the yielded grains had higher amounts of the direct reducing sugars, sucrose, starch; lower contents of total soluble proteins and the total free amino acids, and altered protein patterns compared to those of the uninoculated-drought-stressed plants. SDS-PAGE of the yielded grains showed that drought led to the appearance of some newly synthesized stress protein bands and disappearance of others. Inoculation with PGPB resulted in the re-appearance of some of the disappearing bands and the synthesis of new others. Meanwhile, wheat inoculation under normal conditions resulted in significantly promoted grain yields with higher contents of carbohydrates, total soluble proteins and total free amino acids than that of the uninoculated control. It has been proved that treating wheat plants with the PEG-tolerant Azospirillum brasilense NO40 or Stenotrophomonas maltophilia that were compatible with the systems into which they were introduced and possess multiple plant growth promoting traits, can be an efficient strategy to enhance wheat growth and productivity, not only under normal conditions, but also under drought stress.

Free Amino Acids in Potato Tubers Altered by 2,4-D Treatment of Plants

Science ◽  
1951 ◽  
Vol 114 (2956) ◽  
pp. 204-205
Author(s):  
Merle G. Payne ◽  
Jess L. Fults ◽  
Ruth J. Hay
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers

scholarly journals Effect of daily light integral treatments on free amino acids and sugars contributing flavor and acrylamide formation in potato tubers of Solanum tuberosum L.

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
Minna Rotola-Pukkila ◽  
Anna-Liisa Välimaa ◽  
Jukka-Pekka Suomela ◽  
Baoru Yang ◽  
Saila Karhu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Solanum Tuberosum L ◽  
Free Sugar ◽  
Potato Tubers ◽  
Glucose Content ◽  
Daily Light Integral ◽  
Acrylamide Formation ◽  
Light Integral

To study the effect of photoperiodic conditions on the chemical composition of potato tubers, seven cultivars, grown under controlled conditions, were evaluated for the content of free amino acids (FAA) and sugars. The differences in these compounds may have an effect on the susceptibility of acrylamide formation during potato processing as well as on the flavor profile of potato products. Tubers were produced in growth chambers under two artificially induced photoperiods; 8 h light and 15 h light per day, resulting in conditions with two different daily light integral (DLI) levels. The photoperiodic treatments influenced the total FAA and free sugar contents and composition. Of the analyzed 19 FAAs, the concentrations of 14 FAAs were significantly lower in tubers exposed to the 15 h light period compared to 8 h light, whereas the glucose content was significantly higher. The total FAA concentrations were 15–46% lower and the glucose concentrations 6–64% higher in the seven cultivars exposed to the 15 h light conditions than in those grown in 8 h light.

scholarly journals Change in Content of Sugars and Free Amino Acids in Potato Tubers under Short-Term Storage at Low Temperature and the Effect on Acrylamide Level after Frying

2005 ◽  
Vol 69 (7) ◽  
pp. 1232-1238 ◽  
Author(s):  
Akiko OHARA-TAKADA ◽  
Chie MATSUURA-ENDO ◽  
Yoshihiro CHUDA ◽  
Hiroshi ONO ◽  
Hiroshi YADA ◽  
...  
Keyword(s):  
Amino Acids ◽  
Low Temperature ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage

Free Amino Acids in Potato Tubers Altered by 2,4-D Treatment of Plants

Science ◽  
1951 ◽  
Vol 114 (2956) ◽  
pp. 204-205
Author(s):  
Merle G. Payne ◽  
Jess L. Fults ◽  
Ruth J. Hay
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers

Free Amino Acids in Potato Tubers Altered by 2,4-D Treatment of Plants

Science ◽  
1951 ◽  
Vol 114 (2956) ◽  
pp. 204-205 ◽  
Author(s):  
M. G. Payne ◽  
J. L. Fults ◽  
R. J. Hay
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Potato Tubers

Metabolite Profiling of Barley Grain Subjected to Induced Drought Stress: Responses of Free Amino Acids in Differently Adapted Cultivars

2015 ◽  
Vol 63 (16) ◽  
pp. 4252-4261 ◽  
Author(s):  
Alexandra Lanzinger ◽  
Thomas Frank ◽  
Gabriela Reichenberger ◽  
Markus Herz ◽  
Karl-Heinz Engel
Keyword(s):  
Amino Acids ◽  
Drought Stress ◽  
Stress Responses ◽  
Free Amino Acids ◽  
Metabolite Profiling ◽  
Free Amino ◽  
Barley Grain

scholarly journals Bioactive compounds in potatoes: Accumulation under drought stress conditions

2015 ◽  
Vol 5 (3) ◽  
pp. 108 ◽  
Author(s):  
Christina B. Wegener ◽  
Gisela Jansen ◽  
Hans-Ulrich Jürgens
Keyword(s):  
Amino Acids ◽  
Drought Stress ◽  
Water Supply ◽  
Bioactive Compounds ◽  
Crop Production ◽  
Stress Conditions ◽  
Tuber Quality ◽  
Crude Fibre ◽  
Trolox Equivalent ◽  
Soluble Phenols

Background: Potato (Solanum tuberosum) is a valuable source of bioactive compounds. Besides starch, crude fibre, amino acids (AAS), vitamins and minerals, the tubers contain diverse phenolic compounds. These phenolics and AAS confer anti-oxidant protection against reactive oxygen species, tissue damage, and diseases like atherosclerosis, renal failure, diabetes mellitus, and cancer. Climate change and drought stress may become a major risk for crop production worldwide, resulting in reduced access for those who depend on the nutritional value of this staple crop.Objective: The aim of this study is to determine the effect of drought stress on water, lipid soluble antioxidants, anthocyanins (Ac), soluble phenols, proteins, free AAS, peroxidase (POD) and lipid acyl hydrolase activity (LAH) in tuber tissue.Methods: The study was carried out on three potato genotypes comprising one yellow-fleshed cultivar and two purple breeding clones. The plants were grown in pots (from April to September) in a glasshouse with sufficient water supply and under drought stress conditions. After harvest, the tubers of both variants were analysed for antioxidants measured as ascorbic acid (ACE) and Trolox equivalent (TXE) using a photo-chemiluminescent method. Amounts of anthocyanins (Ac), soluble phenols, proteins, as well as POD and LAH activities were analysed using a UV photometer. Finally, free AAS were measured by HPLC.Results: The results revealed that drought stress significantly reduces tuber yield, but has no significant effect on antioxidants, Ac, soluble phenols and POD. Drought stress significantly increased the levels of soluble protein (P < 0.0001) and LAH (P < 0.001). Also, total amounts of Functional Foods in Health and Disease 2015; 5(3): 108-116 Page 109 of 116 free AAS were higher in the drought stressed tubers (+34.2%, on average) than in the tubers grown with a sufficient water supply. Above all, proline was elevated due to drought stress.Key words: anthocyanins, antioxidants, free amino acids, phenols, proteins, tuber quality

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