PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION

2017 ◽  
Vol 19 (3) ◽  
pp. 41-48
Author(s):  
Ay N.V. ◽  
Duy M.V. ◽  
Baatartsogt O. ◽  
Altantsetseg Kh. ◽  
Enkhchimeg V.
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Stage Iv ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Development Stage ◽  
High Survival ◽  
Ms Medium

In vitro seedling offspring of Plantago camtschatica Link was investigated regarding induction of somatic embryogenesis in petiole/leaf explants from shoot tissue and shoot proliferation. The aim of study was to investigate the medium supplemented with suitable concentration of plant growth regulators in order to induce somatic embryogenesis, plant regeneration and shoot multiplication. The results showed that: (i) Petiole/young leaf of immature stem induced the highest ratio of calli induction and compact calli formation on MS medium supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA; (ii) From created calli, somatic embryogenesis could be induced on MS medium supplemented with 1 mgL-1 TDZ or 1 mgL-1 TDZ and 0.5 mgL-1 NAA; (iii) MS medium supplemented with 5-7 mgL-1 BA shown the most effective on shoot development stage; (iv) Rooting of shoot was the best on 1/2 solid MS medium with activated charcoal (2 gL-1), and 0.5-4 mgL-1 NAA; and (v) acclimatization of micropropagated plants could be planted in plastic pots containing a mixture of decayed straw : rice husk ashes, (1:1, v/v), sand : soil (1:1, v/v) or soil, showed a high survival rate and most seedlings grew normally.

scholarly journals Effect of Plant Growth Regulators on Somatic Embryogenesis and Plantlet Development of Turkey Berry (Solanum torvum SW)

2021 ◽  
pp. 1-8
Author(s):  
Ghan Singh Maloth ◽  
Rajinikanth Marka ◽  
Rama Swamy Nanna
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Solanum Torvum ◽  
Plantlet Formation ◽  
Regenerated Plants

In the present study it was reported on direct somatic embryogenesis and plant regeneration from cotyledon and leaf explants of Turkey berry/pea egg plant (Solanum torvum SW), a medicinally important plant. Somatic embryogenesis has several advantages over other routes of in vitro plant regeneration. Somatic embryogenesis was induced directly from cotyledon and leaf explants on MS medium fortified with BAP (0.5 mg/L)+NAA (0.5-6.0 mg/L). High percentage of somatic embryogenesis (90%), maximum number of somatic embryos formation (62±0.18)  along with high percentage (76%) conversion of somatic embryos into bipolar embryos was observed on cotyledon explants in 0.5 mg/L BAP+2.5 mg/L NAA. At the same concentration of BAP (0.5 mg/L)+NAA (2.5 mg/L) also resulted  on the maximum percentage of somatic embryogenesis (92%), the highest number of somatic embryos formation (88±0.15) and the highest percentage (76%) of somatic embryos conversion into bipolar embryos in leaf explants. A mixture of globular, heart and torpedo-shaped embryos were germinated on MS medium supplemented with 0.5 mg/L IAA+1.0-4.0 mg/L BAP. Maximum germination frequency (75±0.14) of somatic embryos and plantlet formation was found in 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The survival rate of regenerated plants after field transfer was recorded to be 75%. These regenerated plants were found morphologically similar to donor plants. The present protocol can be used for conservation of the species and also for genetic transformation experiments in S. torvum.

scholarly journals (291) Plant Regeneration through Direct Somatic Embryogenesis in Homalomena `Emerald Gem'

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1078A-1078
Author(s):  
Qian Zhang ◽  
Jianjun Chen ◽  
Richard J. Henny
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Direct Somatic Embryogenesis ◽  
Induction Medium ◽  
Ms Medium

Homalomena `Emerald Gem' is an important ornamental foliage plant and widely used for interior plantscaping. Current propagation of this cultivar has been primarily carried out through in vitro culture by organogenesis; regeneration through somatic embryogenesis has not been documented. This report describes successful plant regeneration via direct somatic embryogenesis from explants of different organs. Somatic embryos formed at and around the cut surface of petiole, spathe, and peduncle explants. Embryos also appeared at the base between expanded ovaries of the spadix segment, and around midrib of leaf explants. The optimal treatments for somatic embryo occurrence from petiole, spathe, and peduncle explants were MS medium containing 0.2 mg/L NAA or 0.5 mg/L 2, 4-D with 2.0 mg/L CPPU, and for spadix explants were MS medium with 0.5 mg/L PAA and 2.5 mg/L TDZ. Somatic embryos appeared 6 to 8 weeks after culture and formed large embryo clumps in 3 to 4 months. Somatic embryos produced more secondary embryos and geminated on induction medium. Multiple shoot development and plant regeneration occurred from somatic embryo clusters on MS medium without hormone or with 2 mg/L BA and 0.2 mg/L NAA. The regenerated plants grew vigorously after transplanting to a soilless container substrate in a shaded greenhouse.

scholarly journals Somatic Embryogenesis and Plant Regeneration from in-vitro-grown Leaf Explants of Rose

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1378-1380 ◽  
Author(s):  
C.K. Kim ◽  
J.Y. Oh ◽  
J.D. Chung ◽  
A.M. Burrell ◽  
D.H. Byrne
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Formation ◽  
Ms Medium ◽  
Regeneration Frequency

Somatic embryogenesis was initiated from in vitro-grown leaf explants of rose using an induction period of 4 weeks on MS basal medium supplemented with auxin followed by several subcultures on MS basal medium with cytokinin. `4th of July' showed the highest regeneration frequency (24.4%) on 5.3 μm NAA followed by culture on medium containing 18.2 μm zeatin. `Tournament of Roses' produced somatic embryos when cultured for 4 weeks on medium containing dicamba, 2.3 μm followed by three subcultures on medium containing 18.2 μm zeatin. Embryogenic callus matured on MS media containing 0.5 μm NAA, 6.8 μm zeatin, and 2.9 μm GA3. Long-term cultures were established for both cultivars. Somatic embryos germinated on MS medium containing IBA and BA. Silver nitrate (58.8 μm) enhanced shoot formation and germination of somatic embryos. Plants derived from somatic embryos were acclimatized and successfully established in the greenhouse.

scholarly journals Micropropagation of Clerodendrum phlomidis L.F.

2016 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
Neha Srivastava ◽  
Murugan Nataraj ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Axillary Shoot Proliferation ◽  
Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

scholarly journals Transformation of Nicotiana alata Link and Otto. with an Autoregulatory Senescence-inhibitor Gene Construct

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 617c-617
Author(s):  
Kenneth R. Schroeder ◽  
Dennis P. Stimart
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Nicotiana Alata ◽  
Ms Medium ◽  
Gene Encoding ◽  
Half Strength ◽  
Cytokinin Synthesis ◽  
Senesced Leaves

Leaf explants of Nicotiana alata Link and Otto. were surface disinfested and cultured on Murashige and Skoog (MS) medium containing 2.66 μm N6-benzyladenine (BA) to promote shoot proliferation. After 5 weeks, proliferated shoots were removed and remaining callus saved. Callus was inoculated with Agrobacterium tumefaciens encoding a senescence-specific promoter SAG12 cloned from Arabidopsis thaliana fused to a Agrobacterium tumefaciens gene encoding isopentenyl transferase which catalyzes cytokinin synthesis. Following inoculation, the callus was cocultivated for 6 days on BA medium. Selection for transgenics was done on BA medium plus 100 mg Kanamycin and 400 mg Ticarcillin (antibiotics) per liter. Proliferating shoots were rooted on MS medium containing antibiotics. Rooted cuttings were transplanted to soil, acclimated and flowered in the greenhouse. Transgenics were outcrossed to a commercial N. alata hybrid. Seed was germinated in vitro on half-strength MS medium plus antibiotics. Segregation of transgenics to nontransgenics was 1:1. Evaluation of leaf senescence on 5-month-old plants showed 2 to 14 times fewer senesced leaves on the transgenic than the nontransgenic plants.

In Vitro Shoot Regeneration of NAA-Pulse Treated Plumular Leaf Explants of Cowpea

2010 ◽  
Vol 2 (2) ◽  
pp. 60-63 ◽  
Author(s):  
Muhammad AASIM
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Regeneration ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Grain Legume ◽  
Ms Medium ◽  
Pulse Treatment ◽  
Mature Embryos ◽  
Legume Crop

Cowpea (Vigna unguiculata L.) is an economically important grain legume crop and is an important source of dietary protein in many of the developing countries. The present study reports the effect of pulse treatment duration, concentration of NAA and presence of NAA in the culture medium on shoot regeneration from plumular leaf explant of Turkish cowpea cv. ‘Akkiz’ and ‘Karagoz’. Pulse treatment of mature embryos with 20 mg l-1 NAA for 1 and 3 weeks followed by culturing of plumular leaf explant on MS medium containing 0.25, 0.50 and 1.0 BAP with 1.0, 2.0 and 4.0 mg l-1 NAA promoted somatic embryogenesis in both cultivars. Longer duration of pulse treatment was deleterious resulting in browning and consequently death of the embryos on explants. Pulse treatment with 20 mg l-1 NAA for one week was less deleterious and developed two plantlets after the explants were transferred to MS0 medium after 6 weeks through somatic embryogenesis in cv. ‘Akkiz’. Pulse treatment with 10 mg l-1 NAA for 1 week showed 33.33-50.00% and 25.00-50.00% shoot regeneration frequency in cv. ‘Akkiz’ and ‘Karagoz’ respectively on MS medium containing 0.25-1.00 mg l-1 BAP. Maximum number of 2.50 shoots each per explant were recorded in cv. ‘Akkiz’ and ‘Karagoz’ on MS medium containing 1.00 and 0.50 mg l-1 BAP respectively. Contrarily, maximum shoot length of 8.98 cm of cv. ‘Akkiz’ and 9.42 cm of cv. ‘Karagoz’ was recorded on MS medium containing 0.50 mg l-1 BAP and 1.00 mg l-1 BAP respectively. Regenerated shoots were rooted on MS medium containing 0.5 mg l-1 IBA and and acclimatized in growth room at room temperature where they produced viable seeds.

scholarly journals Micropropagation and Assessment of Genetic Stability of Acclimatized Streptocarpus x hybridus Voss Plantlets Using RAPD Markers

2018 ◽  
Vol 75 (2) ◽  
Author(s):  
Monica HÂRŢA ◽  
Doina CLAPA ◽  
Orsolya BORSAI ◽  
Mihai Călin RUSU ◽  
Cristina KELEMEN ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
High Rate ◽  
Root Induction ◽  
Shoot Number ◽  
Indole 3 Acetic Acid ◽  
Very High

A micropropagation protocol via direct shoot organogenesis from Streptocarpus x hybridus Voss. leaf explants was established in this study. The shoot induction of three Streptocarpus cultivars (‘Snow White’, ‘Black Panther’ and ‘Slumber Song’) was successfully achieved on Murashige and Skoog (MS) medium supplemented with 0.2 mg L-1 -indole-3-acetic acid (IAA) and 0.2 mg L-1 thidiazuron (TDZ). In proliferation stage, the effects of two combinations of plant growth regulators -PGR- (V1-0.2 mg/L-1 IAA + 0.5 mg/L-1 BAP and V2-1.0 mg L-1 NAA + 0.2 mg L-1 TDZ) on shoot number and length were examined. The results suggest that PGRs combinations significantly influenced shoot proliferation and root induction in all Streptocarpus cultivars. Among the treatments, 0.2 mg L-1 (IAA) in combination with 0.5 mg L-1 6-benzylaminopurine (BAP) were the most effective for in vitro shoot multiplication and rooting. The in vitro rooting percentage was also determined before subjecting the plantlets to the acclimatization process. Due to acclimatization, Streptocarpus plantlets showed a very high rate of survival (90%). The generated PCR-RAPD profiles for the selected in vitro-raised plants and donor plants were similar which indicates the clonal or true-to-type nature of the progenies.

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

scholarly journals (281) Micropropagation of Trifoliate Orange Rootstock (Poncirus trifoliate Raf.)

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1052C-1052 ◽  
Author(s):  
Abdelrahman Al-Wasel
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Trifoliate Orange ◽  
Mature Trees ◽  
Poncirus Trifoliate

In vitro propagation of trifoliate orange rootstock (Poncirus trifoliate Raf.) was achieved using axillary buds taken from new flushes of mature trees and then cultured on Murashige and Skoog medium (MS). The addition of growth regulators [0.5 mg·L-1 gibberellic acid (GA3) or 0.1 mg·L-1 6-benzyladenine (BA) and 0.1 mg·L-1 indol-3-butyric acid (IBA)] were necessary to promote bud breakage and shoot elongation. Shoot proliferation was induced on MS medium supplemented with various levels of BA (0.0, 0.5, 1.0, 1.5, and 2.0 mg·L-1) and α-naphthalene acetic acid (NAA) (0.0, 0.1, and 0.5 mg·L-1). Maximal shoot multiplication (9.3 shoots/explant) and elongation (2.3 cm) occurred on media containing either 1.0 mg·L-1 BA alone or with 0.1 mg·L-1 NAA. Shoots rooted better and gave high root number (7.6 roots/shoot) and long roots (5.4 cm) when cultured on a liquid MS medium provided by 0.1 mg·L-1 NAA. Rooted shoots were successfully established in soil (≥90%).

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