An in vitro study of pcDNA 3.0-hVEGF165 gene transfection in endothelial progenitor cells derived from peripheral blood of rabbits

2012 ◽  
Vol 6 (5) ◽  
pp. 270-275 ◽  
Author(s):  
Xiulian Gu
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Gene Transfection ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor

scholarly journals Effect and Mechanism of Thrombospondin-1 on the Angiogenesis Potential in Human Endothelial Progenitor Cells: An In Vitro Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88213 ◽  
Author(s):  
Qing Qin ◽  
Juying Qian ◽  
Lei Ge ◽  
Li Shen ◽  
Jianguo Jia ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor ◽  
Thrombospondin 1

Labeling and Qualification of Endothelial Progenitor Cells for Tracking in Tissue Engineering: An in Vitro Study

2015 ◽  
Vol 38 (4) ◽  
pp. 224-232 ◽  
Author(s):  
Noélie B. Thébaud ◽  
Audrey Aussel ◽  
Robin Siadous ◽  
Jérome Toutain ◽  
Reine Bareille ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor

scholarly journals EFFECT OF FLAVONOIDS ON OXIDATIVE STRESS, APOPTOSIS, AND CELL MARKERS OF PERIPHERAL BLOOD-DERIVED ENDOTHELIAL PROGENITOR CELLS: AN IN VITRO STUDY

2021 ◽  
pp. 39-42
Author(s):  
WAHYU WIDOWATI ◽  
RIMONTA F. GUNANEGARA ◽  
TERESA LILIANA WARGASETIA ◽  
HANNA SARI WIDYA KUSUMA ◽  
SEILA ARUMWARDANA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
In Vitro Study ◽  
Endothelial Progenitor ◽  
Peripheral Blood Mononuclear ◽  
Cell Markers

Objective: Circulating EPCs (endothelial progenitor cells) play a role in neovascularization and vascular repair. Oxidative stress impairs endothelial progenitor. Flavonoid is a phytochemical compound for antioxidant activity. Flavonoid effects toward oxidative stress, apoptosis, and expression of the cell markers on EPCs are not fully understood. This study was aimed to elucidate the effects of quercetin, kaempferol, and myricetin toward oxidative stress, apoptosis, and cell markers of peripheral blood-derived-EPCs. Methods: EPCs (endothelial progenitor cells) were isolated from peripheral blood mononuclear cells (PBMNCs) using cultivation under EPCs spesific media. Oxidative stress in EPCs was induced by H2O2 and then treated by quercetin, kaempferol, and myricetin. Cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, while intracellular reactive oxygen species (ROS), apoptosis and characterization of cells, which expressed CD133 and KDR, was measured using flow cytometry. Results: Quercetin, kaempferol, and myricetin at concentration 12.50 µmol/l were not toxic on EPCs as the cells viability were 96.11±4.03%, 95.42±7.75%, and 94.22±9.49%, respectively. Flavonoids decreased intracellular ROS level in EPCs (quercetin: 14.38±1.47%, kaempferol: 20.21±6.25%, and myricetin: 13.88±4.02%) compared to EPCs treated with H2O2 (30.70%±1.04). Percetage of EPCs apoptosis was not significantly different among each treatment. Immunophenotyping showed the increasing of CD133 and KDR expression in EPCs treated with flavonoids. Conclusion: Quercetin, kaempferol, and myricetin were safe for EPCs, decreased ROS levels, and increased CD133 and KDR expression. However, the flavonoids did not significantly affect EPCs apoptosis.

Influence of porcine-derived collagen matrix on endothelial progenitor cells: an in vitro study

Odontology ◽  
2014 ◽  
Vol 104 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Andreas Max Pabst ◽  
Karl-Martin Lehmann ◽  
Christian Walter ◽  
Maximilian Krüger ◽  
Stefan-Ioan Stratul ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Collagen Matrix ◽  
Vitro Study ◽  
Endothelial Progenitor

scholarly journals In vitro study of human endothelial progenitor cells behaviour on nitrided Ni-free Ti–27Nb alloy

2019 ◽  
Vol 29 (4) ◽  
pp. 466-471 ◽  
Author(s):  
Raluca Ion ◽  
Yvan Bédouin ◽  
Thierry Gloriant ◽  
Gladiola Andruseac ◽  
Doina-Margareta Gordin ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor

scholarly journals Effects of xuezhikang on proliferation and adhesion capacity of cultured endothelial progenitor cells: An in vitro study

2008 ◽  
Vol 69 (3) ◽  
pp. 252-259 ◽  
Author(s):  
Xiang-Quan Kong ◽  
Meng-Zan Wang ◽  
Le-Xin Wang ◽  
Jing-Bo Kong ◽  
Xue-Wen Qi ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor

scholarly journals Adult endothelial progenitor cells from human peripheral blood maintain monocyte/macrophage function throughout in vitro culture

Cell Research ◽  
2006 ◽  
Vol 16 (6) ◽  
pp. 577-584 ◽  
Author(s):  
Shi Ju Zhang ◽  
Hao Zhang ◽  
Ying Jie Wei ◽  
Wen Jun Su ◽  
Zhong Kai Liao ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Endothelial Progenitor ◽  
Macrophage Function

Development of a multi-arm polyrotaxanes modified mesoporous silica-coated gold nanoplatform for protecting endothelial progenitor cells against high glucose environment

2021 ◽  
pp. 088532822110412
Author(s):  
Duanwen Cao ◽  
Huihua Zuo ◽  
Mingjin Jiang ◽  
Yiteng Huang ◽  
Liang Li ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Laser Irradiation ◽  
Endothelial Progenitor Cells ◽  
High Glucose ◽  
Gene Transfection ◽  
In Vitro Studies ◽  
Endothelial Progenitor ◽  
Cell Internalization ◽  
Diabetic Macroangiopathy

Recent study reported that endothelial progenitor cells (EPCs) have potential to treat diabetic macroangiopathy. High glucose environment of diabetes can affect the adhesion of EPCs by decreasing the expression of CXC chemokine receptor 4 (CXCR4) and affect the proliferation of EPCs by decreasing the expression of miR-126. The results showed that the cytotoxicity of GNR@MSNs@PEI to EPCs was significantly lower than PEI; the temperature of GNR@MSNs@PEI solution can be controlled between 38–40°C under 808 nm laser irradiation. 25.67 µg of pcDNA3.1-GFP-CXCR4 and 5.36 µg of FITC-miR-126 could be loaded in 1 mg of GNR@MSNs@PEI; GNR@MSNs@PEI has gene transfection almost the same as Lipofectamine 3000. Subsequent in vitro studies showed that pcDNA3.1-GFP-CXCR4 and miR-126 loaded GNR@MSNs@PEI can significantly increase the adhesion and proliferation and decrease the apoptosis of EPCs treated with high glucose under 808 nm laser irradiation. In conclusion, nano-carriers (GNR@MSNs@PEI) with high pcDNA3.1-CXCR4 and miR-126 loading capacity, high biocompatibility, well cell internalization, and controllable release ability were constructed to transfer CXCR4 expression plasmid (pcDNA3.1-CXCR4) and miR-126 into EPCs efficiently. Further in vitro studies indicated that pcDNA3.1-CXCR4 and miR-126-loaded GNR@MSNs@PEI could protect EPCs against high glucose-induced injury.

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