scholarly journals The influence of chitin synthesis inhibitors on growth of entomopathogenic fungi in vitro

2014 ◽  
Vol 27 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Anna Sapieha ◽  
Ryszard Miętkiewski

<i>Beauveria bassiana, Paecilomyces farinosus, Verticillium lecanii, Conidiobolus thnmboides, Erynia pieris, E. radicans</i> were examined on medium to which were added three chitin synthesis inhibitors.

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).


1999 ◽  
Vol 34 (8) ◽  
pp. 1497-1503 ◽  
Author(s):  
Marcos Rodrigues de Faria ◽  
Irene Martins ◽  
Raquel Mello ◽  
Myrian Silvana Tigano

Twenty four strains of the entomopathogenic fungi (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus and P. lilacinus, maintained in the culture collection of Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) and preserved by lyophilization and in liquid nitrogen, had their conidial viability assessed. Germination rates of 16- to 84-month-old cultures stored in liquid nitrogen decreased, on average, less than 13.3%. For 29- to 49-month-old cultures preserved by lyophilization, the viability loss ranged, on average, from 28.6 to 94.5%. The results demonstrated the efficiency of the tested methods, especially liquid nitrogen, in preserving the viability of entomopathogenic fungi.


2014 ◽  
Vol 25 (2) ◽  
pp. 35-50 ◽  
Author(s):  
Ryszard Miętkiewski ◽  
Anna Sapiecha ◽  
Zofia Miętkiewska

<i>Beauceria bassiana</i> (Bals.) Vuill., <i>Paecilomyces farinosus</i> (Dicks: Fr.) brown et Smith, <i>Verticillium lecanii</i> (Zimm.) Viegas, <i>Conidiobolus thromboides</i> Drechsler, <i>Erynia pieris</i> Li et Humber, <i>E. radicans</i> (Brcf.) Humber were examined on medium to which were added ten herbicides, one fungicide and one insecticide.


2019 ◽  
Vol 11 (17) ◽  
pp. 167
Author(s):  
Vanessa P. Gonçalves ◽  
Cândida R. J. de Farias ◽  
Victoria Moreira-Nunêz ◽  
Renata Moccellin ◽  
Viviana Gaviria-Hernández ◽  
...  

The objective of this work was to evaluate the fungitoxic effect of the agrochemicals used in the cultivation of soybean and irrigated rice on entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae by means of the mycelial growth, sporulation and spore germination. The isolates were inoculated in potato-dextrose-agar (PDA) medium containing the pesticides and exposed to spraying with the products. It was observed that chlorantraniliprole produced the best results regarding and was compatible with the two methods of contact of the product with the fungi. Flubendiamide it&rsquo;s very toxic to fungi, producing 100% inhibition when incorporated into the medium, when by spraying, the fungus M. anisopliae got mycelial growth. Etofenprox and thiamethoxam changed their toxity classification according to the method of contact with product, was moderately compatible to B. bassiana and compatible to M. anisopliae and spinosad showed more compatibility with M. anisopliae than B. bassiana and was classified as compatible. Among fungicides the tricyclazole was the only compatible with the fungus by the spray method. The results showed that in vitro agrochemicals such as tricyclazole, thiamethoxam, flubendiamide and etofenprox are harmful to fungi. It was found that environmental interference could minimize the effects on organisms, especially when the chemicals are applied by spraying.


Author(s):  
Shawky Mohamed Aboelhadid ◽  
Samar Mahmoud Ibrahium ◽  
Walid Mahmoud Arafa ◽  
Lilian Nagy Maahrous ◽  
Abdel-Azeem Shaban Abdel-Baki ◽  
...  

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.


2018 ◽  
Vol 615 ◽  
pp. 1126-1132 ◽  
Author(s):  
Xia Ning ◽  
Tingting Ku ◽  
Rui Gao ◽  
Xiaotong Ji ◽  
Guangke Li ◽  
...  

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