In vitro Efficacy of Verticillium lecanii and Beauveria bassiana of Commercial Source Against Cattle Tick, Rhipicephalus (Boophilus) annulatus

Background and Aim: Ivermectin (IVM) has been used in veterinary practice to control different parasitic infestations over the past two decades. This study aimed to re-assess the acaricidal effects of IVM, as well as to evaluate its efficacy against Rhipicephalus (Boophilus) annulatus by determining the mortality rate, γ-aminobutyric acid (GABA) level, and oxidative/ antioxidative homeostasis (malondialdehyde [MDA] levels and glutathione S-transferase [GST] activities). Materials and Methods: Adult females of Rhipicephalus (Boophilus) annulatus were picked from cattle farms in El-Beheira Governorate, Egypt. Ticks were equally allocated to seven experimental groups to assess the acaricidal potential of IVM chemotherapeutics in controlling R. (B.) annulatus. IVM was prepared at three concentrations (11.43, 17.14, and 34.28 μM of IVM). Results: Mortality rate was calculated among the treated ticks. In addition, GABA, GST, and MDA biomarker levels were monitored. The data revealed a noticeable change in GST activity, a detoxification enzyme found in R. (B.) annulatus, through a critical elevation in mortality percentage. Conclusion: IVM-induced potent acaricidal effects against R. (B.) annulatus by repressing GST activity for the initial 24 h after treatment. Collectively, this paper reports the efficacy of IVM in a field population of R. (B.) annulatus in Egypt. Keywords: acaricidal, glutathione S-transferase, ivermectin, malondialdehyde, Rhipicephalus (Boophilus) annulatus, γ-aminobutyric acid.

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In vitro acaricidal effects of ethanolic extract and its fractions of Ageratum conyzoides L. against common cattle tick, Rhipicephalus (Boophilus) annulatus

Annals of Phytomedicine An International Journal ◽

10.21276/ap.2017.6.2.17 ◽

2017 ◽

Vol 6 (2) ◽

Author(s):

Suresh Narayanan Nair ◽

Sanis Juliet ◽

Krishnan Kavillimakkil Amithamol ◽

Athalathil Ramankutty Sunil ◽

Adarsh Krishna Thumadath Palayullaparambil ◽

...

Keyword(s):

Ethanolic Extract ◽

Cattle Tick ◽

Ageratum Conyzoides ◽

Boophilus Annulatus

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The influence of chitin synthesis inhibitors on growth of entomopathogenic fungi in vitro

Acta Mycologica ◽

10.5586/am.1992.016 ◽

2014 ◽

Vol 27 (2) ◽

pp. 189-195 ◽

Cited By ~ 1

Author(s):

Anna Sapieha ◽

Ryszard Miętkiewski

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Verticillium Lecanii ◽

Chitin Synthesis ◽

Chitin Synthesis Inhibitors ◽

Paecilomyces Farinosus

Beauveria bassiana, Paecilomyces farinosus, Verticillium lecanii, Conidiobolus thnmboides, Erynia pieris, E. radicans were examined on medium to which were added three chitin synthesis inhibitors.

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Biocontrol Potential of Some Entomopathogenic Fungal Strains Against Bean Aphid Megoura japonica (Matsumura)

Agriculture ◽

10.3390/agriculture10040114 ◽

2020 ◽

Vol 10 (4) ◽

pp. 114

Author(s):

Duy Nam Trinh ◽

Thi Kim Lien Ha ◽

Dewen Qiu

Keyword(s):

Beauveria Bassiana ◽

Culture Filtrate ◽

Post Treatment ◽

Verticillium Lecanii ◽

Fungal Strains ◽

Virulence Potential ◽

Conidial Concentration ◽

Biocontrol Potential ◽

Binary Combination

This research reported the in vitro pathogenicity of Verticillium lecanii strains, L2 and L5, and Beauveria bassiana strains, B76 and B252, against Megoura japonica using leaf-dip method. The virulence potential of these four entomopathogenic fungal strains of V. lecanii and of B. bassiana were compared between fungi conidia (concentrations 1 × 106, 1 × 107, and 1 × 108 conidia mL−1) and culture filtrate. Moreover, binary combination of four different fungal strains (L2 + B76, B76 + L5, L2 + B252, and L2 + B76 + B252 + L5) were evaluated against M. japonica under control condition. Aphid mortality was recorded after two, four, six, and eight days of post-treatment. In the conidial concentration bioassay, strain B76 showed maximal mortality (85.3%) against bean aphid, and strain L5 showed the lowest effect (60.0%) at the highest concentration (1 × 108 conidia mL−1) at eight days post-treatment. Whereas, binary combinations of B76 and L2 strains showed the highest effect against M. japonica (90.5%) than other combinations. Moreover, in comparison with the effect of filtrate and conidia bioassay, 91.4% and 84.1% were achieved in strain B76, and the lowest effect (63.8% and 55.1%) was recorded in strain L5.

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Virulence of Aerial Conidia of Beauveria bassiana Produced under LED Light to Ctenocephalides felis (Cat Flea)

Journal of Pathogens ◽

10.1155/2018/1806830 ◽

2018 ◽

Vol 2018 ◽

pp. 1-4

Author(s):

Sarayut Pittarate ◽

Malee Thungrabeab ◽

Supamit Mekchay ◽

Patcharin Krutmuang

Keyword(s):

Beauveria Bassiana ◽

Adult Mortality ◽

Ctenocephalides Felis ◽

Fluorescent Light ◽

Malt Extract ◽

Led Light ◽

Red Led ◽

Aerial Conidia ◽

Different Color

Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis. The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups. All of the treatments with B. bassiana conidia caused adult mortality after an exposure of 12 h. Among the conditions used in this study, B. bassiana cultured under red LED and fluorescent light were the most effective in causing mortality (100 %) in adult fleas after 36 h. The experimental results indicate that these aerial conidia of B. bassiana have promising potential for use in control of C. felis adult stages.

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In vitro transcriptomes analysis identifies some special genes involved in pathogenicity difference of the Beauveria bassiana against different insect hosts

Microbial Pathogenesis ◽

10.1016/j.micpath.2021.104824 ◽

2021 ◽

Vol 154 ◽

pp. 104824

Author(s):

Jing Liu ◽

Ziqi Ling ◽

Jingjie Wang ◽

Tingting Xiang ◽

Liang Xu ◽

...

Keyword(s):

Beauveria Bassiana

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In vitro activity of the hydroalcoholic extract of Chenopodium ambrosioides against engorged females of Rhipicephalus (Boophilus) microplus

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000222016 ◽

2018 ◽

Vol 84 (0) ◽

Author(s):

Eduardo Oliveira ◽

Manoela da Silva ◽

Lew Sprenger ◽

Daniela Pedrassani

Keyword(s):

High Efficiency ◽

Boophilus Microplus ◽

Vitro Activity ◽

Economic Losses ◽

Cattle Tick ◽

Distilled Water ◽

In Vitro Activity ◽

Chenopodium Ambrosioides ◽

Cattle Ticks

ABSTRACT: Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.

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Controle associado de Alphitobius diaperinuse efeito de microrganismos eficazes no desenvolvimento de Beauveria bassiana

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008000100001 ◽

2008 ◽

Vol 43 (1) ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Patricia Helena Santoro ◽

Pedro Manuel Oliveira Janeiro Neves ◽

Silvia Akimi Cavaguchi ◽

Kelly Constanski ◽

Junio Tavares Amaro ◽

...

Keyword(s):

Beauveria Bassiana ◽

Alphitobius Diaperinus

O objetivo deste trabalho foi avaliar a eficiência de Beauveria bassiana (Bb), terra diatomácea (TD) e microrganismos eficazes (EM-4), associados ou não, no controle de Alphitobius diaperinus, e o efeito de EM-4 no desenvolvimento de B. bassiana. Os agentes de controle (Bb, TD e EM-4), em diferentes concentrações e combinações, foram aplicados em uma mistura de cama-de-frango e ração, em que os insetos foram acondicionados por dez dias. Avaliaram-se, in vitro: a germinação, as unidades formadoras de colônia (UFC), o crescimento vegetativo e a produtividade de conídios de Bb em contato com suspensão aquosa de EM-4 (1%) não filtrada e filtrada. Os maiores índices de mortalidade foram observados nos tratamentos TD + Bb + EM-4 e TD + Bb, nas maiores concentrações. Verificou-se efeito não-aditivo sinérgico para TD + Bb nas três concentrações. Nos testes com EM-4 filtrado, não houve diferença em relação à testemunha quanto à germinação e às UFC, entretanto, o crescimento vegetativo e a produtividade de conídios foram negativamente afetados. O uso conjunto de B. bassiana e terra diatomácea, para o manejo de populações de A. diaperinus, pode reduzir o uso de produtos químicos.

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Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132001000400013 ◽

2001 ◽

Vol 44 (4) ◽

pp. 419-423 ◽

Cited By ~ 29

Author(s):

Edson Hirose ◽

Pedro M. O. J. Neves ◽

João A. C. Zequi ◽

Luís H. Martins ◽

Cristiane H. Peralta ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Azadirachta Indica ◽

Organic Agriculture ◽

Metarhizium Anisopliae ◽

Neem Oil ◽

Colony Diameter ◽

Negative Effect ◽

Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion Ô and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas MultibionÔ caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

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