scholarly journals DNA synthesis in the nuclei of Pinus silvestris embryos during

2015 ◽  
Vol 43 (4) ◽  
pp. 459-464
Author(s):  
P. Brodzki

DNA synthesis starts earliest in the apical meristem of the shoot, and latest in the cotyledons. Mitoses appear simultaneously in the apical meristem and in the hypocotyl cortex. Synthesis continues in the mother cells of vascular elements and cotyledon parenchyma when mitosis ceases. In the cotyledons DNA synthesis is rather synchronous and leads to the elimination of 2 C nuclei.

1982 ◽  
Vol 60 (11) ◽  
pp. 2197-2214 ◽  
Author(s):  
Hardev Singh ◽  
John N. Owens

Phenology and anatomy of the postdormancy reproductive phase of Abies grandis Lindl, were studied. The dormant microsporangia contained compactly arranged pollen mother cells (PMC). The pollen cones broke dormancy in the 3rd week of February and soon afterwards the PMC entered meiosis. Microspore tetrads formed by the 2nd week of March. Pollen grains were shed at the five-celled stage in the 3rd week of April. The pollen grains were bisaccate and showed a triradiate mark on the proximal pole. The dormant ovulate-cone buds bore rudimentary ovuliferous scales, each with two ovular areas. Ovulate cones broke dormancy at the end of January. Megaspore mother cells differentiated by the end of February and the integument was initiated soon afterwards. A megaspore triad formed in the 2nd week of April. By the 3rd week of April, at the time of pollination, the ovule contained a free-nuclear gametophyte, and the integument had developed a stigmatic micropylar funnel. Numerous microdroplets were observed on the surface of the funnel to which pollen adhered. After pollination the funnel became infolded, enclosing the pollen grains. Pollen germination, pollen tube growth through the nucellus, and syngamy took only 3–4 days and occurred in the 3rd week of June. The female gametophyte was long and bore two or three archegonia. The proembryo consisted of four tiers of four cells each. The suspensors developed from the subterminal tier of cells. The four terminal cells formed the embryonal mass, whose proximal cells elongated and developed into a secondary suspensor. Differentiation of the root apical meristem and the cotyledons in the young embryo occurred in the 1st week of July and the embryo matured in the 3rd week of August.


2000 ◽  
Vol 27 (12) ◽  
pp. 1109
Author(s):  
Marie-Noëlle Jordy ◽  
Susanna Danti ◽  
Jean-Michel Favre ◽  
Milvia Luisa Raccchi

The spatio-temporal evolution of catalase (CAT) activity and triacylglycerol distribution was investigated in seeds and seedlings from Pinus pinaster Ait., P. pinea L. and P. radiata D. Don during germination and post-germination. The high amount of triacylglycerols contained in the whole dehydrated embryo from the three species was progressively depleted, first, in the radicle and then in hypocotyl and cotyledons during post-germinative growth. In parallel, histological localisation of CAT activity and the quantitative analysis confirmed the involvement of this enzyme in cell detoxification from peroxide released during the intense lipid breakdown. Two isozymes, CAT-1 and CAT-2, were identified during post-germinative growth. Both were particularly active in the hypocotyl and radicle, while CAT-2 was specifically active in the photosynthetic tissues. These results emphasise that CAT activity is also independent from lipid metabolism in certain tissues. The role of each isoenzyme is discussed in connection with the metabolic changes occurring during seed germination and seedling growth. Special attention is given to the role of the shoot apex in triacylglycerol storage and breakdown. Central mother cells have been shown as a specific lipid storage area of the shoot apical meristem, in contrast with the peripheral zone in which lipid reserves were always reduced.


Botany ◽  
2015 ◽  
Vol 93 (7) ◽  
pp. 445-452
Author(s):  
Subramanian Paulraj ◽  
Arturo Lopez-Villalobos ◽  
Edward C. Yeung

The formation of meristemoids and the ontogeny of the shoot apical meristem (SAM) were studied using cultured zygotic embryos of Arabidopsis thaliana (L.) Heynh. LER ecotype. In the callus induction treatment, the procambial cells within the cotyledons of the embryo explants proliferated and gave rise to callus tissues. At the end of the treatment, a band of small cytoplasmic-rich cells derived from the procambium was produced and located at the outer surface of the callus. Upon transfer to the shoot induction medium (SIM) in the absence of abscisic acid (ABA), the cytoplasmic cells differentiated mainly into vascular elements and vacuolated parenchyma cells. This pattern of development negatively affected the explants’ ability to produce meristemoids and SAMs. Contrary to the control, the inclusion of ABA in the SIM resulted first in starch synthesis and accumulation in the surface cytoplasmic cells. This was followed by the formation of cytoplasmic cells among the starch-rich cells; further proliferation of the cytoplasmic cells resulted in the formation of meristemoids. The formation of tracheary elements was suppressed in the ABA-containing SIM. Upon transferring to the shoot development medium, which lacked plant growth regulators, some meristemoids differentiated into apical meristem cells. These cells had distinct nuclei and nucleoli, with little starch present. Additional cell divisions increased the size of the future SAM. Shoot buds with distinct SAMs were clearly delineated with the appearance of leaf primordia.


1982 ◽  
Vol 56 (1) ◽  
pp. 293-302
Author(s):  
D. R. SMYTH

About three-quarters of the DNA synthesis occurring in pachytene pollen mother cells of Lilium henryi takes place in proplastids. Only around 15% can be attributed to mitochondrial labelling and 10% to nuclear DNA synthesis. Label was identified in the proplastid genome by its location in electron microscopic autoradiographs, by its buoyant density (1.698 g/ml), and by its specific hybridization to chloroplast DNA sequences from spinach. Proplastids, while apparently not dividing at pachytene, may be replicating their DNA in readiness for subsequent proliferation in developing microspores. The annealing properties of plastid DNA closely parallel those of labelled pachytene DNA sequences implicated in meiotic exchange events.


1997 ◽  
Vol 110 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Ritsuko Koitabashi ◽  
Takeshi Suzuki ◽  
Tamotsu Kawazu ◽  
Atsushi Sakai ◽  
Haruko Kuroiwa ◽  
...  

RADIOISOTOPES ◽  
1991 ◽  
Vol 40 (10) ◽  
pp. 418-420
Author(s):  
Atsushi TATARA ◽  
Abdul Aziz BARADJINEGARA ◽  
Hikoyuki YAMAGUCHI

1987 ◽  
Vol 65 (2) ◽  
pp. 280-285 ◽  
Author(s):  
J. M. Harris ◽  
A. P. Singh

Short shoots and the needles that they bear usually persist for about 3 years on stems and branches of Pinus radiata D. Don. As the main axis thickens with secondary growth, short shoots may elongate by four times their original length, during which process xylem elements become embedded in the stem as "needle trances," although the external shoots appear to remain unchanged. This report describes how elongation in both xylem and phloem results from the insertion of new vascular elements derived from marginal cells of radial parenchyma bands. There was no evidence that the apical meristem contributes to this growth. Possible mechanisms for elongation of pith and cortex are also described.


1995 ◽  
Vol 46 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Takeshi Suzuki ◽  
Narie Sasaki ◽  
Atsushi Sakai ◽  
Shigeyuki Kawano ◽  
Tsuneyoshi Kuroiwa

2015 ◽  
Vol 48 (3) ◽  
pp. 391-421 ◽  
Author(s):  
Teresa Tykarska

It was found in the continued studies on rape embryogenesis, started by the description of the proembryo (Tykarska, 1976) that the development of embryo is extremely regular and based on differentiating divisions. It appeared that the transverse segmentation boundary and cell walls separating the mother cells of the histogens in the proembryo can be distinguished in all the later stages of the embryo. The border between the cytoledons and epicotyl part of the embryonal axis, and the hypocotyl corresponds to the segmentation boundary between layer l and layer l' at the octant stage. As border between the hypocotyl and radicle was assumed the upper boundary of the root cap reaching usually to the level of the boundary between segments II and III of dermatogen and periblem. The apical meristem of the shoot forms from dermatogen and the periaxial cells of the globular embryo subepidermis. The promeristem of the radicle constists of 3 layers of initial cells surrounding on all sides the inactive layer of central binding cells.


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