scholarly journals Somatic embryogenesis in in vitro culture of Plantago asiatica L.

2014 ◽  
Vol 69 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Joanna Makowczyńska ◽  
Emilia Andrzejewska-Golec
Keyword(s):  
Somatic Embryogenesis ◽  
Light Microscopy ◽  
In Vitro Culture ◽  
Direct Somatic Embryogenesis ◽  
Early Stages ◽  
Plantago Asiatica ◽  
Somatic Organogenesis

Early stages of direct somatic embryogenesis in <em>Plantago asiatica</em> were observed by light microscopy. In calli of <em>Plantago asiatica</em> two phenomena occurred simultaneously: somatic organogenesis and somatic embryogenesis.

In vitro culture and somatic embryogenesis of four Trifolium species

Plant Science ◽  
1986 ◽  
Vol 45 (2) ◽  
pp. 101-104 ◽  
Author(s):  
G.A. Pederson
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture

Direct somatic embryogenesis and encapsulation of somatic embryos for in vitro conservation of Bacopa monnieri (L.) Wettst

2016 ◽  
Vol 127 (2) ◽  
pp. 433-442 ◽  
Author(s):  
Barkha Khilwani ◽  
Amanpreet Kaur ◽  
Ritika Ranjan ◽  
Anil Kumar
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Bacopa Monnieri ◽  
In Vitro Conservation ◽  
Direct Somatic Embryogenesis

Somatic embryogenesis in in vitro culture of three larch species

2012 ◽  
Vol 43 (6) ◽  
pp. 353-361 ◽  
Author(s):  
I. N. Tret’yakova ◽  
A. V. Barsukova
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Larch Species

scholarly journals High expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE coincides with initiation of various developmental pathways in in vitro culture of Trifolium nigrescens

PROTOPLASMA ◽  
2015 ◽  
Vol 253 (2) ◽  
pp. 345-355 ◽  
Author(s):  
Maria Pilarska ◽  
Przemysław Malec ◽  
Jan Salaj ◽  
Filip Bartnicki ◽  
Robert Konieczny
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
High Expression ◽  
Developmental Pathways ◽  
Receptor Like Kinase ◽  
Trifolium Nigrescens

scholarly journals Somatic Embryogenesis and Organogenesis in Magnolia dealbata Zucc. (Magnoliaceae), an Endangered, Endemic Mexican Species

HortScience ◽  
2006 ◽  
Vol 41 (5) ◽  
pp. 1325-1329 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Ángel Jiménez-Rodríguez ◽  
Victor M. Chávez-Avila
Keyword(s):  
Somatic Embryogenesis ◽  
Basal Medium ◽  
Direct Somatic Embryogenesis ◽  
Direct Organogenesis ◽  
Limiting Factor ◽  
Zygotic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Magnolia Dealbata

Plants of Magnolia dealbata were regenerated from zygotic embryos through somatic embryogenesis and direct organogenesis. Medium and incubation conditions were determinating factors for the development of morphogenetic responses. Photoperiodic exposure was a limiting factor in the general development of the explants, and incubation in darkness allowed their development. The highest formation of shoots per responding explant were obtained on woody plant (WP) medium supplemented with 13.3 μM or 22.2 μM 6-benzylaminopurine (BA) in combination with 2.26 μM or in absence of 2,4-dichlorophenoxyacetic acid (2,4-D) from which 2.5 shoots per explant were induced. Subcultures on WP medium, supplemented with polyvinylpyrrolidone (PUP) 40,000 1 g·L–1) avoided necrosis of explants. Somatic embryos were formed in 85% of explants cultivated on WP medium with 2,4-D (2.3 μM or 4.5 μM); 20% induced indirect embryogenesis and 65% formed direct somatic embryogenesis. The plants were transferred to soil to acclimatize under greenhouse conditions, achieving 90% survival. Somatic embryo conversion to plantlets was obtained with subculture on WP basal medium without growth regulators. In vitro culture can play a key role in the propagation and conservation of this endangered species.

scholarly journals Cytohistological analysis of somatic embryogenesis in cucumber (Cucumis sativus L). II. Natural fluorescence and direct somatic embryogenesis from protoplasts

2014 ◽  
Vol 63 (3-4) ◽  
pp. 265-268 ◽  
Author(s):  
W. Burza ◽  
B. Woźniak ◽  
J. A. Tarkowska ◽  
S. Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
Protoplast Culture ◽  
Direct Somatic Embryogenesis ◽  
Histological Structure ◽  
Cucumis Sativus L ◽  
Natural Fluorescence ◽  
Vacuolated Cells ◽  
Selection Of

The development of protoplast derived from somatic embryos and some of their characteristics were compared with embryos from suspension and in vivo in the same B line. Embryos formed in a protoplast culture differed from others that their younger stages contained vacuolated cells, and older ones had altered morphological and histological structure. Somatic embryogenesis is more regular from suspension then from protoplasts. No distinct differences were observed in the rate of embryo development in vivo and in vitro, and in vitro embryos show a larger variation in size at the same stage. Embryos in vitro with fluorescence are generally larger than zygotic ones at each stage. The use of fluorescence is suggested for the selection of heterokariocytes after protoplast fusion.

In Vitro Culture of Wheat and Rice for Understanding the Molecular Basis of Somatic Embryogenesis and for Transformation

1990 ◽  
pp. 191-213 ◽  
Author(s):  
N. Maheshwari ◽  
K. Rajyalakshmi ◽  
C. N. Chowdhry ◽  
A. Grover ◽  
A. K. Tyagi ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Molecular Basis

THE EFFICIENT INDUCTION OF EMBRYOGENIC CALLUS AND HIGH FREQUENT SOMATIC EMBRYOGENESIS OF IN VITRO CULTURE OF BER

2007 ◽  
pp. 205-212
Author(s):  
J.-X. Fu ◽  
C.-M. Liu ◽  
G.-B. Hu ◽  
J.-N. Tan ◽  
G.-D. Zhang
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Embryogenic Callus ◽  
Efficient Induction

Extracellular Matrix in Early Stages of Direct Somatic Embryogenesis in Leaves of Drosera spathulata

2003 ◽  
Vol 46 (2) ◽  
pp. 161-166 ◽  
Author(s):  
M. Bobak ◽  
J. Samaj ◽  
E. Hlinkova ◽  
A. Hlavacka ◽  
M. Ovecka
Keyword(s):  
Extracellular Matrix ◽  
Somatic Embryogenesis ◽  
Direct Somatic Embryogenesis ◽  
Early Stages
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