Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
Keyword(s):
Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.
2009 ◽
Vol 34
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pp. 3288-3294
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1973 ◽
Vol 45
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pp. 287-291
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1987 ◽
Vol 45
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pp. 622-623
2016 ◽
Vol 63
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pp. 343-350
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1973 ◽
Vol 30
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pp. 334-338
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2012 ◽
Vol 26
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pp. 740-746
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