scholarly journals Reexamination of Dopaminergic Amacrine Cells in the Rabbit Retina: Confocal Analysis with Doubleand Triple-labeling Immunohistochemistry

2017 ◽  
Vol 26 (6) ◽  
pp. 329-338
Author(s):  
Jong Woo Lee ◽  
Min Young Lim ◽  
Yong Soo Park ◽  
Su Jin Park ◽  
In-Beom Kim
Keyword(s):  
Amacrine Cells ◽  
Rabbit Retina

Comparison of the responses of AII amacrine cells in the dark- and light-adapted rabbit retina

1999 ◽  
Vol 16 (4) ◽  
pp. 653-665 ◽  
Author(s):  
DAIYAN XIN ◽  
STEWART A. BLOOMFIELD
Keyword(s):  
Receptive Field ◽  
Light Adaptation ◽  
Amacrine Cells ◽  
Bipolar Cells ◽  
Rabbit Retina ◽  
Response Component ◽  
Synaptic Inputs ◽  
Field Organization ◽  
Receptive Field Organization ◽  
Aii Amacrine Cells

We studied the light-evoked responses of AII amacrine cells in the rabbit retina under dark- and light-adapted conditions. In contrast to the results of previous studies, we found that AII cells display robust responses to light over a 6–7 log unit intensity range, well beyond the operating range of rod photoreceptors. Under dark adaptation, AII cells showed an ON-center/OFF-surround receptive-field organization. The intensity–response profile of the center-mediated response component followed a dual-limbed sigmoidal function indicating a transition from rod to cone mediation as stimulus intensities were increased. Following light adaptation, the receptive-field organization of AII cells changed dramatically. Light-adapted AII cells showed both ON- and OFF-responses to stimulation of the center receptive field, but we found no evidence for an antagonistic surround. Interestingly, the OFF-center response appeared first following rapid light adaptation and was then replaced gradually over a 1–4 min period by the emerging ON-center response component. Application of the metabotropic glutamate receptor agonist APB, the ionotropic glutamate blocker CNQX, 8-bromo-cGMP, and the nitric oxide donor SNAP all showed differential effects on the various center-mediated responses displayed by dark- and light-adapted AII cells. Taken together, these pharmacological results indicated that different synaptic circuits are responsible for the generation of the different AII cell responses. Specifically, the rod-driven ON-center responses are apparently derived from rod bipolar cell synaptic inputs, whereas the cone-driven ON-center responses arise from signals crossing the gap junctions between AII cells and ON-center cone bipolar cells. Additionally, the OFF-center response of light-adapted AII cells reflects direct synaptic inputs from OFF-center cone bipolar cells to AII dendritic processes in the distal inner plexiform layer.

NADPH-diaphorase (nitric oxide synthase)-reactive amacrine cells of rabbit retina: Putative target cells and stimulation by light

Neuroscience ◽  
1993 ◽  
Vol 57 (3) ◽  
pp. 587-597 ◽  
Author(s):  
J. Koistinaho ◽  
R.A. Swanson ◽  
J. de Vente ◽  
S.M. Sagar
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Amacrine Cells ◽  
Nadph Diaphorase ◽  
Target Cells ◽  
Rabbit Retina ◽  
Putative Target ◽  
Oxide Synthase

Symmetric synaptic patterns between starburst amacrine cells and direction selective ganglion cells in the rabbit retina

2008 ◽  
Vol 508 (1) ◽  
pp. 175-183 ◽  
Author(s):  
Yung-Cheng Chen ◽  
Chuan-Chin Chiao
Keyword(s):  
Ganglion Cells ◽  
Amacrine Cells ◽  
Rabbit Retina ◽  
Starburst Amacrine Cells

scholarly journals Cholinergic amacrine cells of the rabbit retina contain glutamate decarboxylase and gamma-aminobutyrate immunoreactivity.

1988 ◽  
Vol 85 (16) ◽  
pp. 6187-6191 ◽  
Author(s):  
N. Brecha ◽  
D. Johnson ◽  
L. Peichl ◽  
H. Wassle
Keyword(s):  
Glutamate Decarboxylase ◽  
Amacrine Cells ◽  
Rabbit Retina ◽  
Cholinergic Amacrine Cells

Dendritic maturation of displaced putative cholinergic amacrine cells in the rabbit retina

1989 ◽  
Vol 287 (2) ◽  
pp. 164-178 ◽  
Author(s):  
R. O. L. Wong ◽  
S. P. Collin
Keyword(s):  
Amacrine Cells ◽  
Rabbit Retina ◽  
Cholinergic Amacrine Cells

Neural architecture of the “transient” ON directionally selective (class IIb1) ganglion cells in rabbit retina, partly co-stratified with starburst amacrine cells

2016 ◽  
Vol 33 ◽  
Author(s):  
EDWARD V. FAMIGLIETTI
Keyword(s):  
T Cells ◽  
Ganglion Cell ◽  
Ganglion Cells ◽  
Amacrine Cells ◽  
Dendritic Morphology ◽  
Inner Plexiform Layer ◽  
Rabbit Retina ◽  
Branching Pattern ◽  
Stimulus Velocity ◽  
Starburst Amacrine Cells

AbstractRecent physiological studies coupled with intracellular staining have subdivided ON directionally selective (DS) ganglion cells of rabbit retina into two types. One exhibits more “transient” and more “brisk” responses (ON DS-t), and the other has more “sustained’ and more “sluggish” responses (ON DS-s), although both represent the same three preferred directions and show preference for low stimulus velocity, as reported in previous studies of ON DS ganglion cells in rabbit retina. ON DS-s cells have the morphology of ganglion cells previously shown to project to the medial terminal nucleus (MTN) of the accessory optic system, and the MTN-projecting, class IVus1 cells have been well-characterized previously in terms of their dendritic morphology, branching pattern, and stratification. ON DS-t ganglion cells have a distinctly different morphology and exhibit heterotypic coupling to amacrine cells, including axon-bearing amacrine cells, with accompanying synchronous firing, while ON DS-s cells are not coupled. The present study shows that ON DS-t cells are morphologically identical to the previously well-characterized, “orphan” class IIb1 ganglion cell, previously regarded as a member of the “brisk-concentric” category of ganglion cells. Its branching pattern, quantitatively analyzed, is similar to that of the morphological counterparts of X and Y cells, and very different from that of the ON DS-s ganglion cell. Close analysis of the dendritic stratification of class IIb1 ganglion cells together with fiducial cells indicates that they differ from that of the ON DS-s cells. In agreement with one of the three previous studies, class IIb1/ON DS-t cells, unlike class IVus1/ON DS-s ganglion cells, in the main do not co-stratify with starburst amacrine cells. As the present study shows, however, portions of their dendrites do deviate from the main substratum, coming within range of starburst boutons. Parsimony favors DS input from starburst amacrine cells both to ON DS-s and to ON DS-t ganglion cells, given the similarity of their DS responses, but further studies will be required to substantiate the origin of the DS responses of ON DS-t cells. Previously reported OFF DS responses in ON DS-t cells, unmasked by pharmacological agents, and mediated by gap junctions with amacrine cells, suggests an unusual trans-sublaminar organization of directional selectivity in the inner plexiform layer, connecting sublamina a and sublamina b.

scholarly journals AMPA receptors mediate acetylcholine release from starburst amacrine cells in the rabbit retina

2003 ◽  
Vol 466 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Sally I. Firth ◽  
Wei Li ◽  
Stephen C. Massey ◽  
David W. Marshak
Keyword(s):  
Ampa Receptors ◽  
Acetylcholine Release ◽  
Amacrine Cells ◽  
Rabbit Retina ◽  
Starburst Amacrine Cells

GABA inhibits ACh release from the rabbit retina: A direct effect or feedback to bipolar cells?

1992 ◽  
Vol 8 (2) ◽  
pp. 97-106 ◽  
Author(s):  
David M. Linn ◽  
Stephen C. Massey
Keyword(s):  
Direct Effect ◽  
Negative Feedback ◽  
Bipolar Cell ◽  
Amacrine Cells ◽  
Bipolar Cells ◽  
Rabbit Retina ◽  
Ach Release ◽  
Gaba Antagonists ◽  
Direct Input ◽  
Cholinergic Amacrine Cells

AbstractThe cholinergic amacrine cells of the rabbit retina may be labeled with [3H]-Ch and the activity of the cholinergic population monitored by following the release of [3H]-ACh. We have tested the effect of muscimol, a potent GABAA agonist, on (1) the light-evoked release of ACh, presumably mediated via bipolar cells, which are known to have a direct input to the cholinergic amacrine cells and (2) ACh release produced by exogenous glutamate analogs that probably have a direct effect on cholinergic amacrine cells. Muscimol blocked the light-evoked release of ACh with an IC50 of 1.0 μM. In contrast, ACh release produced by nonsaturating doses of kainate or NMDA was not reduced even by 100 μM muscimol. Thus, we have been unable to demonstrate a direct effect of GABA on the cholinergic amacrine cells.GABA antagonists, such as picrotoxin, caused a large increase in the base release and potentiated the light-evoked release of ACh. Both these effects were abolished by DNQX, a kainate antagonist that blocks the input to cholinergic amacine cells from bipolar cells. DNQX blocked the effects of picrotoxin even when controls showed that the mechanism of ACh release was still functional. Together, these results imply that the dominant site for the GABA-mediated inhibition of ACh release is on the bipolar cell input to the cholinergic amacrine cells. This is consistent with previous anatomical and physiological evidence that bipolar cells receive negative feedback from GABA amacrine cells.

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